Experiment: LB, pH6
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMc03847 and SMc03848 overlap by 4 nucleotides SMc03848 and SMc03849 are separated by 59 nucleotides SMc03849 and SMc03850 overlap by 4 nucleotides SMc03850 and SMc03851 overlap by 4 nucleotides SMc03851 and SMc03852 are separated by 158 nucleotides
SMc03847: SMc03847 - cytochrome c biogenesis protein CcmA, at 3,513,863 to 3,514,486
SMc03847
SMc03848: SMc03848 - heme exporter B (cytochrome C-type biogenesis protein) transmembrane, at 3,514,483 to 3,515,142
SMc03848
SMc03849: SMc03849 - heme exporter C (cytochrome C-type biogenesis protein) transmembrane, at 3,515,202 to 3,515,972
SMc03849
SMc03850: SMc03850 - heme exporter D (cytochrome C-type biogenesis protein) transmembrane, at 3,515,969 to 3,516,145
SMc03850
SMc03851: SMc03851 - thiol:disulfide interchange protein (cytochrome C biogenesis protein), at 3,516,142 to 3,516,750
SMc03851
SMc03852: SMc03852 - hypothetical protein, at 3,516,909 to 3,517,502
SMc03852
Position (kb) 3515
3516 Strain fitness (log2 ratio) -2
-1
0
1 at 3515.063 kb on + strand, within SMc03848 at 3515.158 kb on + strand at 3515.631 kb on + strand, within SMc03849 at 3515.785 kb on + strand, within SMc03849 at 3515.963 kb on + strand at 3515.963 kb on + strand at 3515.987 kb on + strand, within SMc03850 at 3515.988 kb on - strand, within SMc03850 at 3516.511 kb on + strand, within SMc03851 at 3516.748 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB, pH6 remove 3,515,063 + SMc03848 0.88 -1.2 3,515,158 + +0.1 3,515,631 + SMc03849 0.56 -0.1 3,515,785 + SMc03849 0.76 -1.8 3,515,963 + -2.3 3,515,963 + +0.8 3,515,987 + SMc03850 0.10 -0.8 3,515,988 - SMc03850 0.11 -2.6 3,516,511 + SMc03851 0.61 -1.7 3,516,748 + +0.4
Or see this region's nucleotide sequence