Experiment: LB, pH6
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b21633 and SM_b21634 are separated by 33 nucleotides SM_b21634 and SM_b21635 overlap by 4 nucleotides SM_b21635 and SM_b21636 are separated by 50 nucleotides
SM_b21633: SM_b21633 - enoyl-CoA hydratase, at 1,661,661 to 1,662,452
_b21633
SM_b21634: SM_b21634 - acyl-CoA thioester hydrolase, at 1,662,486 to 1,662,989
_b21634
SM_b21635: SM_b21635 - bifunctional aldehyde dehydrogenase/enoyl-CoA hydratase, at 1,662,986 to 1,665,052
_b21635
SM_b21636: SM_b21636 - ferredoxin reductase electron transfer protein, at 1,665,103 to 1,666,179
_b21636
Position (kb)
1662
1663
1664
1665
1666 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1662.393 kb on + strand at 1662.487 kb on + strand at 1662.664 kb on + strand, within SM_b21634 at 1662.751 kb on + strand, within SM_b21634 at 1662.751 kb on + strand, within SM_b21634 at 1662.752 kb on - strand, within SM_b21634 at 1662.839 kb on - strand, within SM_b21634 at 1663.025 kb on + strand at 1663.025 kb on + strand at 1663.025 kb on + strand at 1663.025 kb on + strand at 1663.025 kb on + strand at 1663.026 kb on - strand at 1663.026 kb on - strand at 1663.026 kb on - strand at 1663.026 kb on - strand at 1663.026 kb on - strand at 1663.033 kb on - strand at 1663.159 kb on - strand at 1663.159 kb on - strand at 1663.195 kb on - strand, within SM_b21635 at 1663.204 kb on - strand, within SM_b21635 at 1663.260 kb on + strand, within SM_b21635 at 1663.260 kb on + strand, within SM_b21635 at 1663.333 kb on + strand, within SM_b21635 at 1663.333 kb on + strand, within SM_b21635 at 1663.334 kb on - strand, within SM_b21635 at 1663.539 kb on + strand, within SM_b21635 at 1663.540 kb on - strand, within SM_b21635 at 1663.796 kb on - strand, within SM_b21635 at 1663.800 kb on + strand, within SM_b21635 at 1663.801 kb on - strand, within SM_b21635 at 1663.875 kb on + strand, within SM_b21635 at 1663.875 kb on + strand, within SM_b21635 at 1664.288 kb on - strand, within SM_b21635 at 1664.605 kb on + strand, within SM_b21635 at 1664.606 kb on - strand, within SM_b21635 at 1664.703 kb on + strand, within SM_b21635 at 1664.784 kb on + strand, within SM_b21635 at 1664.892 kb on + strand at 1664.892 kb on + strand at 1664.893 kb on - strand at 1664.893 kb on - strand at 1665.010 kb on + strand at 1665.012 kb on + strand at 1665.114 kb on - strand at 1665.165 kb on - strand at 1665.165 kb on - strand at 1665.182 kb on + strand at 1665.182 kb on + strand at 1665.183 kb on - strand at 1665.741 kb on - strand, within SM_b21636 at 1665.741 kb on - strand, within SM_b21636 at 1665.787 kb on + strand, within SM_b21636 at 1666.011 kb on - strand, within SM_b21636
Per-strain Table
Position Strand Gene LocusTag Fraction LB, pH6 remove 1,662,393 + -0.0 1,662,487 + +0.9 1,662,664 + SM_b21634 0.35 -1.0 1,662,751 + SM_b21634 0.53 -1.2 1,662,751 + SM_b21634 0.53 +0.5 1,662,752 - SM_b21634 0.53 -1.2 1,662,839 - SM_b21634 0.70 -0.2 1,663,025 + -0.7 1,663,025 + +0.0 1,663,025 + +0.5 1,663,025 + -0.5 1,663,025 + +1.4 1,663,026 - -0.8 1,663,026 - +0.2 1,663,026 - +1.2 1,663,026 - +0.2 1,663,026 - -1.6 1,663,033 - +0.7 1,663,159 - -0.2 1,663,159 - -1.9 1,663,195 - SM_b21635 0.10 -1.6 1,663,204 - SM_b21635 0.11 +0.8 1,663,260 + SM_b21635 0.13 -0.2 1,663,260 + SM_b21635 0.13 -0.4 1,663,333 + SM_b21635 0.17 +0.3 1,663,333 + SM_b21635 0.17 -0.3 1,663,334 - SM_b21635 0.17 -0.8 1,663,539 + SM_b21635 0.27 -0.8 1,663,540 - SM_b21635 0.27 +0.1 1,663,796 - SM_b21635 0.39 -0.8 1,663,800 + SM_b21635 0.39 -0.0 1,663,801 - SM_b21635 0.39 -2.1 1,663,875 + SM_b21635 0.43 +0.5 1,663,875 + SM_b21635 0.43 -0.5 1,664,288 - SM_b21635 0.63 -0.8 1,664,605 + SM_b21635 0.78 -0.1 1,664,606 - SM_b21635 0.78 +1.7 1,664,703 + SM_b21635 0.83 +0.4 1,664,784 + SM_b21635 0.87 -0.5 1,664,892 + -0.1 1,664,892 + +1.9 1,664,893 - -0.5 1,664,893 - +0.7 1,665,010 + -0.1 1,665,012 + +4.3 1,665,114 - +0.5 1,665,165 - +0.3 1,665,165 - +1.5 1,665,182 + -0.9 1,665,182 + -1.3 1,665,183 - -1.0 1,665,741 - SM_b21636 0.59 -0.0 1,665,741 - SM_b21636 0.59 -0.2 1,665,787 + SM_b21636 0.64 -0.1 1,666,011 - SM_b21636 0.84 +1.3
Or see this region's nucleotide sequence