Experiment: LB, pH6
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b21328 and SM_b21329 are separated by 58 nucleotides SM_b21329 and SM_b21330 are separated by 215 nucleotides SM_b21330 and SM_b21331 are separated by 231 nucleotides SM_b21331 and SM_b21332 are separated by 273 nucleotides SM_b21332 and SM_b21333 are separated by 54 nucleotides
SM_b21328: SM_b21328 - Sir2-like transcriptional silencer protein, at 997,274 to 998,092
_b21328
SM_b21329: SM_b21329 - hypothetical protein, at 998,151 to 998,321
_b21329
SM_b21330: SM_b21330 - hypothetical protein, at 998,537 to 998,746
_b21330
SM_b21331: SM_b21331 - hypothetical protein, at 998,978 to 999,298
_b21331
SM_b21332: SM_b21332 - hypothetical protein, at 999,572 to 1,000,069
_b21332
SM_b21333: SM_b21333 - hypothetical protein, at 1,000,124 to 1,000,483
_b21333
Position (kb) 998
999
1000 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 998.132 kb on - strand at 998.152 kb on + strand at 998.153 kb on - strand at 998.303 kb on + strand, within SM_b21329 at 998.303 kb on + strand, within SM_b21329 at 998.304 kb on - strand, within SM_b21329 at 998.314 kb on + strand at 998.314 kb on + strand at 998.422 kb on + strand at 998.532 kb on + strand at 998.533 kb on - strand at 998.697 kb on + strand, within SM_b21330 at 998.698 kb on - strand, within SM_b21330 at 998.720 kb on + strand, within SM_b21330 at 998.836 kb on + strand at 998.837 kb on - strand at 998.887 kb on + strand at 998.887 kb on + strand at 998.887 kb on + strand at 998.887 kb on + strand at 998.888 kb on - strand at 998.888 kb on - strand at 998.888 kb on - strand at 999.011 kb on - strand, within SM_b21331 at 999.011 kb on - strand, within SM_b21331 at 999.248 kb on + strand, within SM_b21331 at 999.248 kb on + strand, within SM_b21331 at 999.249 kb on - strand, within SM_b21331 at 999.250 kb on + strand, within SM_b21331 at 999.344 kb on - strand at 999.344 kb on - strand at 999.500 kb on + strand at 999.501 kb on - strand at 999.627 kb on - strand, within SM_b21332 at 999.627 kb on - strand, within SM_b21332 at 999.680 kb on - strand, within SM_b21332 at 999.707 kb on + strand, within SM_b21332 at 999.708 kb on - strand, within SM_b21332 at 999.708 kb on - strand, within SM_b21332 at 999.708 kb on - strand, within SM_b21332 at 1000.141 kb on - strand at 1000.141 kb on - strand at 1000.141 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB, pH6 remove 998,132 - -0.3 998,152 + -0.4 998,153 - -0.8 998,303 + SM_b21329 0.89 -0.2 998,303 + SM_b21329 0.89 -0.3 998,304 - SM_b21329 0.89 -0.4 998,314 + +0.9 998,314 + -1.2 998,422 + -0.2 998,532 + +0.0 998,533 - -2.0 998,697 + SM_b21330 0.76 +0.5 998,698 - SM_b21330 0.77 -0.1 998,720 + SM_b21330 0.87 -0.8 998,836 + -2.1 998,837 - +0.1 998,887 + +1.2 998,887 + +0.1 998,887 + +2.0 998,887 + +0.5 998,888 - +0.5 998,888 - +0.6 998,888 - +1.4 999,011 - SM_b21331 0.10 -2.4 999,011 - SM_b21331 0.10 -2.2 999,248 + SM_b21331 0.84 -1.0 999,248 + SM_b21331 0.84 -1.2 999,249 - SM_b21331 0.84 -0.4 999,250 + SM_b21331 0.85 -0.3 999,344 - -1.5 999,344 - -0.5 999,500 + +0.0 999,501 - -0.0 999,627 - SM_b21332 0.11 -0.2 999,627 - SM_b21332 0.11 +0.3 999,680 - SM_b21332 0.22 +0.6 999,707 + SM_b21332 0.27 +0.3 999,708 - SM_b21332 0.27 -1.0 999,708 - SM_b21332 0.27 +0.1 999,708 - SM_b21332 0.27 +0.2 1,000,141 - -0.9 1,000,141 - -3.0 1,000,141 - +2.7
Or see this region's nucleotide sequence