Experiment: LB, pH6
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b20404 and SM_b20405 are separated by 12 nucleotides SM_b20405 and SM_b20406 are separated by 127 nucleotides SM_b20406 and SM_b20407 are separated by 8 nucleotides
SM_b20404: SM_b20404 - aldehyde dehydrogenase, at 416,859 to 419,099
_b20404
SM_b20405: SM_b20405 - transcriptional regulator, at 419,112 to 419,891
_b20405
SM_b20406: SM_b20406 - 5-oxoprolinase, at 420,019 to 422,133
_b20406
SM_b20407: SM_b20407 - 5-oxoprolinase, at 422,142 to 424,343
_b20407
Position (kb) 420
421
422
423 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 419.060 kb on + strand at 419.182 kb on + strand at 419.183 kb on - strand at 419.183 kb on - strand at 419.183 kb on - strand at 419.530 kb on - strand, within SM_b20405 at 419.581 kb on + strand, within SM_b20405 at 419.581 kb on + strand, within SM_b20405 at 419.676 kb on + strand, within SM_b20405 at 419.677 kb on - strand, within SM_b20405 at 419.680 kb on + strand, within SM_b20405 at 419.680 kb on + strand, within SM_b20405 at 419.680 kb on + strand, within SM_b20405 at 419.680 kb on + strand, within SM_b20405 at 419.681 kb on - strand, within SM_b20405 at 419.683 kb on + strand, within SM_b20405 at 419.683 kb on + strand, within SM_b20405 at 419.684 kb on - strand, within SM_b20405 at 419.684 kb on - strand, within SM_b20405 at 419.687 kb on + strand, within SM_b20405 at 419.687 kb on + strand, within SM_b20405 at 419.688 kb on - strand, within SM_b20405 at 419.762 kb on - strand, within SM_b20405 at 419.808 kb on - strand, within SM_b20405 at 419.901 kb on + strand at 419.901 kb on + strand at 419.966 kb on + strand at 419.966 kb on + strand at 419.966 kb on + strand at 419.966 kb on + strand at 419.967 kb on - strand at 419.967 kb on - strand at 419.997 kb on + strand at 419.998 kb on - strand at 420.168 kb on + strand at 420.212 kb on - strand at 420.335 kb on - strand, within SM_b20406 at 420.335 kb on - strand, within SM_b20406 at 420.335 kb on - strand, within SM_b20406 at 420.458 kb on - strand, within SM_b20406 at 420.552 kb on - strand, within SM_b20406 at 420.619 kb on + strand, within SM_b20406 at 420.619 kb on + strand, within SM_b20406 at 420.621 kb on + strand, within SM_b20406 at 420.621 kb on + strand, within SM_b20406 at 420.688 kb on + strand, within SM_b20406 at 420.723 kb on + strand, within SM_b20406 at 420.741 kb on + strand, within SM_b20406 at 420.741 kb on + strand, within SM_b20406 at 420.742 kb on - strand, within SM_b20406 at 420.866 kb on - strand, within SM_b20406 at 420.866 kb on - strand, within SM_b20406 at 420.888 kb on + strand, within SM_b20406 at 420.888 kb on + strand, within SM_b20406 at 420.930 kb on + strand, within SM_b20406 at 421.077 kb on - strand, within SM_b20406 at 421.192 kb on + strand, within SM_b20406 at 421.192 kb on + strand, within SM_b20406 at 421.192 kb on + strand, within SM_b20406 at 421.280 kb on + strand, within SM_b20406 at 421.281 kb on - strand, within SM_b20406 at 421.347 kb on + strand, within SM_b20406 at 421.510 kb on + strand, within SM_b20406 at 421.510 kb on + strand, within SM_b20406 at 421.511 kb on - strand, within SM_b20406 at 421.511 kb on - strand, within SM_b20406 at 421.512 kb on + strand, within SM_b20406 at 421.512 kb on + strand, within SM_b20406 at 421.513 kb on - strand, within SM_b20406 at 421.699 kb on + strand, within SM_b20406 at 421.798 kb on + strand, within SM_b20406 at 421.798 kb on + strand, within SM_b20406 at 421.799 kb on - strand, within SM_b20406 at 421.799 kb on - strand, within SM_b20406 at 421.799 kb on - strand, within SM_b20406 at 421.960 kb on + strand at 421.960 kb on + strand at 421.961 kb on - strand at 421.961 kb on - strand at 421.961 kb on - strand at 421.961 kb on - strand at 421.962 kb on + strand at 421.963 kb on - strand at 421.994 kb on - strand at 422.119 kb on + strand at 422.227 kb on - strand at 422.227 kb on - strand at 422.304 kb on + strand at 422.305 kb on - strand at 422.388 kb on + strand, within SM_b20407 at 422.485 kb on - strand, within SM_b20407 at 422.542 kb on + strand, within SM_b20407 at 422.543 kb on - strand, within SM_b20407 at 422.543 kb on - strand, within SM_b20407 at 422.591 kb on + strand, within SM_b20407 at 422.592 kb on - strand, within SM_b20407 at 422.592 kb on - strand, within SM_b20407 at 422.751 kb on + strand, within SM_b20407 at 422.755 kb on + strand, within SM_b20407 at 422.755 kb on + strand, within SM_b20407 at 422.756 kb on - strand, within SM_b20407 at 422.790 kb on + strand, within SM_b20407 at 422.848 kb on + strand, within SM_b20407 at 422.880 kb on + strand, within SM_b20407 at 422.881 kb on - strand, within SM_b20407 at 422.986 kb on - strand, within SM_b20407 at 422.986 kb on - strand, within SM_b20407 at 423.046 kb on - strand, within SM_b20407 at 423.046 kb on - strand, within SM_b20407 at 423.046 kb on - strand, within SM_b20407 at 423.124 kb on - strand, within SM_b20407
Per-strain Table
Position Strand Gene LocusTag Fraction LB, pH6 remove 419,060 + -0.8 419,182 + +0.9 419,183 - +1.0 419,183 - -1.1 419,183 - -0.8 419,530 - SM_b20405 0.54 +0.5 419,581 + SM_b20405 0.60 -0.5 419,581 + SM_b20405 0.60 +0.1 419,676 + SM_b20405 0.72 +0.4 419,677 - SM_b20405 0.72 -0.2 419,680 + SM_b20405 0.73 -0.3 419,680 + SM_b20405 0.73 -0.7 419,680 + SM_b20405 0.73 -1.0 419,680 + SM_b20405 0.73 +0.2 419,681 - SM_b20405 0.73 -0.1 419,683 + SM_b20405 0.73 +0.6 419,683 + SM_b20405 0.73 -0.4 419,684 - SM_b20405 0.73 -0.5 419,684 - SM_b20405 0.73 +0.5 419,687 + SM_b20405 0.74 +0.7 419,687 + SM_b20405 0.74 -1.3 419,688 - SM_b20405 0.74 +0.3 419,762 - SM_b20405 0.83 +0.7 419,808 - SM_b20405 0.89 +0.3 419,901 + +0.2 419,901 + -0.5 419,966 + -2.9 419,966 + -1.5 419,966 + +0.3 419,966 + -1.2 419,967 - +0.9 419,967 - +0.2 419,997 + +0.9 419,998 - -2.5 420,168 + -0.1 420,212 - +0.2 420,335 - SM_b20406 0.15 -0.2 420,335 - SM_b20406 0.15 -1.6 420,335 - SM_b20406 0.15 -0.8 420,458 - SM_b20406 0.21 -1.0 420,552 - SM_b20406 0.25 -0.2 420,619 + SM_b20406 0.28 -0.3 420,619 + SM_b20406 0.28 -0.1 420,621 + SM_b20406 0.28 +0.0 420,621 + SM_b20406 0.28 -1.8 420,688 + SM_b20406 0.32 +1.3 420,723 + SM_b20406 0.33 +2.0 420,741 + SM_b20406 0.34 -0.9 420,741 + SM_b20406 0.34 -0.8 420,742 - SM_b20406 0.34 -1.2 420,866 - SM_b20406 0.40 -0.3 420,866 - SM_b20406 0.40 -1.3 420,888 + SM_b20406 0.41 -0.1 420,888 + SM_b20406 0.41 +0.3 420,930 + SM_b20406 0.43 -0.6 421,077 - SM_b20406 0.50 -0.2 421,192 + SM_b20406 0.55 +0.6 421,192 + SM_b20406 0.55 +0.0 421,192 + SM_b20406 0.55 -1.0 421,280 + SM_b20406 0.60 +0.6 421,281 - SM_b20406 0.60 -0.6 421,347 + SM_b20406 0.63 +0.6 421,510 + SM_b20406 0.70 +3.0 421,510 + SM_b20406 0.70 -0.8 421,511 - SM_b20406 0.71 -0.3 421,511 - SM_b20406 0.71 -0.2 421,512 + SM_b20406 0.71 -0.0 421,512 + SM_b20406 0.71 -1.0 421,513 - SM_b20406 0.71 +0.5 421,699 + SM_b20406 0.79 -0.7 421,798 + SM_b20406 0.84 -1.1 421,798 + SM_b20406 0.84 -0.3 421,799 - SM_b20406 0.84 +1.0 421,799 - SM_b20406 0.84 -1.4 421,799 - SM_b20406 0.84 -1.5 421,960 + +0.7 421,960 + -0.2 421,961 - +0.5 421,961 - -0.2 421,961 - +1.5 421,961 - +1.2 421,962 + -1.1 421,963 - -0.5 421,994 - -1.6 422,119 + -0.0 422,227 - +0.9 422,227 - -0.3 422,304 + -0.7 422,305 - +1.4 422,388 + SM_b20407 0.11 +0.0 422,485 - SM_b20407 0.16 +1.1 422,542 + SM_b20407 0.18 -0.1 422,543 - SM_b20407 0.18 +0.9 422,543 - SM_b20407 0.18 -0.2 422,591 + SM_b20407 0.20 -0.3 422,592 - SM_b20407 0.20 +1.1 422,592 - SM_b20407 0.20 +0.0 422,751 + SM_b20407 0.28 +0.9 422,755 + SM_b20407 0.28 -0.4 422,755 + SM_b20407 0.28 -0.1 422,756 - SM_b20407 0.28 -0.4 422,790 + SM_b20407 0.29 -0.3 422,848 + SM_b20407 0.32 -1.7 422,880 + SM_b20407 0.34 -1.1 422,881 - SM_b20407 0.34 +2.0 422,986 - SM_b20407 0.38 -0.2 422,986 - SM_b20407 0.38 +0.8 423,046 - SM_b20407 0.41 +0.5 423,046 - SM_b20407 0.41 +0.7 423,046 - SM_b20407 0.41 -0.2 423,124 - SM_b20407 0.45 -0.7
Or see this region's nucleotide sequence