Experiment: LB, pH6
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS23530 and BPHYT_RS23535 are separated by 190 nucleotides BPHYT_RS23535 and BPHYT_RS23540 are separated by 32 nucleotides BPHYT_RS23540 and BPHYT_RS23545 overlap by 4 nucleotides
BPHYT_RS23530: BPHYT_RS23530 - hypothetical protein, at 846,563 to 846,790
_RS23530
BPHYT_RS23535: BPHYT_RS23535 - ABC transporter substrate-binding protein, at 846,981 to 847,700
_RS23535
BPHYT_RS23540: BPHYT_RS23540 - RND transporter, at 847,733 to 849,241
_RS23540
BPHYT_RS23545: BPHYT_RS23545 - nodulation protein, at 849,238 to 852,558
_RS23545
Position (kb) 847
848
849
850 Strain fitness (log2 ratio) -1
0
1 at 846.877 kb on + strand at 846.882 kb on - strand at 847.433 kb on - strand, within BPHYT_RS23535 at 847.433 kb on - strand, within BPHYT_RS23535 at 848.175 kb on + strand, within BPHYT_RS23540 at 848.175 kb on + strand, within BPHYT_RS23540 at 848.176 kb on - strand, within BPHYT_RS23540 at 849.351 kb on - strand at 849.756 kb on - strand, within BPHYT_RS23545 at 849.812 kb on + strand, within BPHYT_RS23545
Per-strain Table
Position Strand Gene LocusTag Fraction LB, pH6 remove 846,877 + -0.3 846,882 - +1.2 847,433 - BPHYT_RS23535 0.63 -0.0 847,433 - BPHYT_RS23535 0.63 -1.0 848,175 + BPHYT_RS23540 0.29 -0.2 848,175 + BPHYT_RS23540 0.29 -0.1 848,176 - BPHYT_RS23540 0.29 -1.7 849,351 - +0.2 849,756 - BPHYT_RS23545 0.16 -0.2 849,812 + BPHYT_RS23545 0.17 +0.5
Or see this region's nucleotide sequence