Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMc01702 and SMc01701 are separated by 88 nucleotides SMc01701 and SMc02181 are separated by 249 nucleotides
SMc01702: SMc01702 - oxidoreductase, at 509,911 to 510,897
SMc01702
SMc01701: SMc01701 - hypothetical protein, at 510,986 to 511,570
SMc01701
SMc02181: SMc02181 - bifunctional proline dehydrogenase/pyrroline-5-carboxylate dehydrogenase, at 511,820 to 515,521
SMc02181
Position (kb) 510
511
512 Strain fitness (log2 ratio) -2
-1
0
1
2 at 510.340 kb on + strand, within SMc01702 at 510.340 kb on + strand, within SMc01702 at 510.371 kb on + strand, within SMc01702 at 510.372 kb on - strand, within SMc01702 at 510.449 kb on + strand, within SMc01702 at 510.512 kb on + strand, within SMc01702 at 510.512 kb on + strand, within SMc01702 at 510.512 kb on + strand, within SMc01702 at 510.513 kb on - strand, within SMc01702 at 510.601 kb on - strand, within SMc01702 at 510.612 kb on + strand, within SMc01702 at 510.614 kb on + strand, within SMc01702 at 510.614 kb on + strand, within SMc01702 at 510.615 kb on - strand, within SMc01702 at 510.904 kb on + strand at 510.904 kb on + strand at 510.905 kb on - strand at 510.905 kb on - strand at 510.950 kb on + strand at 510.962 kb on + strand at 510.963 kb on - strand at 511.401 kb on + strand, within SMc01701 at 511.402 kb on - strand, within SMc01701 at 511.613 kb on + strand at 511.767 kb on - strand at 511.767 kb on - strand at 511.767 kb on - strand at 511.984 kb on + strand at 512.184 kb on + strand at 512.185 kb on - strand at 512.185 kb on - strand at 512.186 kb on + strand at 512.186 kb on + strand at 512.187 kb on - strand at 512.187 kb on - strand at 512.241 kb on + strand, within SMc02181 at 512.242 kb on - strand, within SMc02181 at 512.443 kb on - strand, within SMc02181 at 512.538 kb on + strand, within SMc02181 at 512.539 kb on - strand, within SMc02181 at 512.558 kb on - strand, within SMc02181
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 510,340 + SMc01702 0.43 -1.8 510,340 + SMc01702 0.43 +1.2 510,371 + SMc01702 0.47 -0.6 510,372 - SMc01702 0.47 +0.4 510,449 + SMc01702 0.55 -0.4 510,512 + SMc01702 0.61 +0.6 510,512 + SMc01702 0.61 -1.1 510,512 + SMc01702 0.61 -0.9 510,513 - SMc01702 0.61 -2.5 510,601 - SMc01702 0.70 -0.5 510,612 + SMc01702 0.71 +0.2 510,614 + SMc01702 0.71 -0.7 510,614 + SMc01702 0.71 -0.0 510,615 - SMc01702 0.71 -0.9 510,904 + +0.7 510,904 + -0.6 510,905 - +0.1 510,905 - +0.0 510,950 + +0.3 510,962 + -0.3 510,963 - -0.6 511,401 + SMc01701 0.71 +0.1 511,402 - SMc01701 0.71 +0.5 511,613 + +0.9 511,767 - -0.3 511,767 - -0.3 511,767 - -0.0 511,984 + +1.4 512,184 + +1.8 512,185 - +2.2 512,185 - +0.3 512,186 + +1.4 512,186 + +1.4 512,187 - +1.0 512,187 - +1.3 512,241 + SMc02181 0.11 +1.6 512,242 - SMc02181 0.11 +1.0 512,443 - SMc02181 0.17 +0.0 512,538 + SMc02181 0.19 +0.7 512,539 - SMc02181 0.19 +0.9 512,558 - SMc02181 0.20 +0.8
Or see this region's nucleotide sequence