Experiment: D-Gluconic Acid (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS31980 and RR42_RS31985 are separated by 11 nucleotides RR42_RS31985 and RR42_RS31990 overlap by 17 nucleotides RR42_RS31990 and RR42_RS31995 are separated by 99 nucleotides RR42_RS31995 and RR42_RS32000 are separated by 58 nucleotides
RR42_RS31980: RR42_RS31980 - 3-ketosteroid-9-alpha-hydroxylase, at 2,552,726 to 2,553,754
_RS31980
RR42_RS31985: RR42_RS31985 - short-chain dehydrogenase, at 2,553,766 to 2,554,533
_RS31985
RR42_RS31990: RR42_RS31990 - delta 4, 5-alpha steroid dehydrogenase, at 2,554,517 to 2,556,223
_RS31990
RR42_RS31995: RR42_RS31995 - flavin reductase, at 2,556,323 to 2,556,841
_RS31995
RR42_RS32000: RR42_RS32000 - 3-oxosteroid 1-dehydrogenase, at 2,556,900 to 2,558,642
_RS32000
Position (kb)
2554
2555
2556
2557 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2554.011 kb on - strand, within RR42_RS31985 at 2554.011 kb on - strand, within RR42_RS31985 at 2554.080 kb on - strand, within RR42_RS31985 at 2554.126 kb on - strand, within RR42_RS31985 at 2554.126 kb on - strand, within RR42_RS31985 at 2554.126 kb on - strand, within RR42_RS31985 at 2554.860 kb on + strand, within RR42_RS31990 at 2554.941 kb on + strand, within RR42_RS31990 at 2554.941 kb on + strand, within RR42_RS31990 at 2554.942 kb on - strand, within RR42_RS31990 at 2554.942 kb on - strand, within RR42_RS31990 at 2554.942 kb on - strand, within RR42_RS31990 at 2554.942 kb on - strand, within RR42_RS31990 at 2555.370 kb on + strand, within RR42_RS31990 at 2555.410 kb on + strand, within RR42_RS31990 at 2555.411 kb on - strand, within RR42_RS31990 at 2555.808 kb on + strand, within RR42_RS31990 at 2555.812 kb on - strand, within RR42_RS31990 at 2555.817 kb on + strand, within RR42_RS31990 at 2555.818 kb on - strand, within RR42_RS31990 at 2555.818 kb on - strand, within RR42_RS31990 at 2555.974 kb on - strand, within RR42_RS31990 at 2556.043 kb on - strand, within RR42_RS31990 at 2556.378 kb on + strand, within RR42_RS31995 at 2556.378 kb on + strand, within RR42_RS31995 at 2556.545 kb on - strand, within RR42_RS31995 at 2556.805 kb on + strand at 2556.806 kb on - strand at 2556.829 kb on + strand at 2556.830 kb on - strand at 2556.858 kb on - strand at 2556.858 kb on - strand at 2556.858 kb on - strand at 2556.858 kb on - strand at 2557.211 kb on - strand, within RR42_RS32000 at 2557.211 kb on - strand, within RR42_RS32000 at 2557.214 kb on + strand, within RR42_RS32000 at 2557.215 kb on - strand, within RR42_RS32000
Per-strain Table
Position Strand Gene LocusTag Fraction D-Gluconic Acid (C) remove 2,554,011 - RR42_RS31985 0.32 -0.3 2,554,011 - RR42_RS31985 0.32 +1.1 2,554,080 - RR42_RS31985 0.41 -0.7 2,554,126 - RR42_RS31985 0.47 -0.9 2,554,126 - RR42_RS31985 0.47 +0.0 2,554,126 - RR42_RS31985 0.47 -1.3 2,554,860 + RR42_RS31990 0.20 +0.4 2,554,941 + RR42_RS31990 0.25 +0.2 2,554,941 + RR42_RS31990 0.25 -2.8 2,554,942 - RR42_RS31990 0.25 -0.4 2,554,942 - RR42_RS31990 0.25 +0.9 2,554,942 - RR42_RS31990 0.25 +0.5 2,554,942 - RR42_RS31990 0.25 +1.3 2,555,370 + RR42_RS31990 0.50 -0.0 2,555,410 + RR42_RS31990 0.52 -0.8 2,555,411 - RR42_RS31990 0.52 +0.2 2,555,808 + RR42_RS31990 0.76 +0.7 2,555,812 - RR42_RS31990 0.76 +0.7 2,555,817 + RR42_RS31990 0.76 +0.2 2,555,818 - RR42_RS31990 0.76 -1.3 2,555,818 - RR42_RS31990 0.76 +0.7 2,555,974 - RR42_RS31990 0.85 +0.2 2,556,043 - RR42_RS31990 0.89 +0.5 2,556,378 + RR42_RS31995 0.11 +0.7 2,556,378 + RR42_RS31995 0.11 +0.7 2,556,545 - RR42_RS31995 0.43 +0.8 2,556,805 + -0.5 2,556,806 - +0.7 2,556,829 + +0.1 2,556,830 - -0.4 2,556,858 - +0.3 2,556,858 - +0.4 2,556,858 - +0.4 2,556,858 - +0.5 2,557,211 - RR42_RS32000 0.18 +0.1 2,557,211 - RR42_RS32000 0.18 +0.4 2,557,214 + RR42_RS32000 0.18 -0.6 2,557,215 - RR42_RS32000 0.18 +0.4
Or see this region's nucleotide sequence