Experiment: D-Gluconic Acid (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS28835 and RR42_RS28840 are separated by 116 nucleotides RR42_RS28840 and RR42_RS28845 are separated by 40 nucleotides RR42_RS28845 and RR42_RS28850 are separated by 2 nucleotides
RR42_RS28835: RR42_RS28835 - gluconate:H+ symporter (gntT) (from data) , at 1,822,732 to 1,824,093
_RS28835
RR42_RS28840: RR42_RS28840 - hypothetical protein, at 1,824,210 to 1,824,602
_RS28840
RR42_RS28845: RR42_RS28845 - D-aminoacylase, at 1,824,643 to 1,826,136
_RS28845
RR42_RS28850: RR42_RS28850 - transcriptional regulator, at 1,826,139 to 1,827,032
_RS28850
Position (kb)
1824
1825
1826
1827 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1823.755 kb on - strand, within RR42_RS28835 at 1823.755 kb on - strand, within RR42_RS28835 at 1823.911 kb on + strand, within RR42_RS28835 at 1823.911 kb on + strand, within RR42_RS28835 at 1823.995 kb on - strand at 1824.059 kb on + strand at 1824.059 kb on + strand at 1824.229 kb on + strand at 1824.508 kb on + strand, within RR42_RS28840 at 1824.508 kb on + strand, within RR42_RS28840 at 1824.508 kb on + strand, within RR42_RS28840 at 1824.508 kb on + strand, within RR42_RS28840 at 1824.509 kb on - strand, within RR42_RS28840 at 1824.596 kb on + strand at 1824.636 kb on + strand at 1824.636 kb on + strand at 1824.636 kb on + strand at 1824.637 kb on - strand at 1824.637 kb on - strand at 1824.637 kb on - strand at 1824.637 kb on - strand at 1824.637 kb on - strand at 1824.637 kb on - strand at 1824.644 kb on + strand at 1824.645 kb on - strand at 1824.848 kb on + strand, within RR42_RS28845 at 1825.092 kb on - strand, within RR42_RS28845 at 1825.092 kb on - strand, within RR42_RS28845 at 1825.092 kb on - strand, within RR42_RS28845 at 1825.268 kb on + strand, within RR42_RS28845 at 1825.268 kb on + strand, within RR42_RS28845 at 1825.275 kb on - strand, within RR42_RS28845 at 1825.371 kb on + strand, within RR42_RS28845 at 1825.371 kb on + strand, within RR42_RS28845 at 1825.371 kb on + strand, within RR42_RS28845 at 1825.371 kb on + strand, within RR42_RS28845 at 1825.372 kb on - strand, within RR42_RS28845 at 1825.547 kb on + strand, within RR42_RS28845 at 1825.736 kb on + strand at 1825.737 kb on - strand, within RR42_RS28845 at 1826.534 kb on - strand, within RR42_RS28850 at 1826.620 kb on + strand, within RR42_RS28850 at 1826.621 kb on - strand, within RR42_RS28850 at 1826.663 kb on + strand, within RR42_RS28850 at 1827.109 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Gluconic Acid (C) remove 1,823,755 - RR42_RS28835 0.75 -3.7 1,823,755 - RR42_RS28835 0.75 -2.5 1,823,911 + RR42_RS28835 0.87 -2.5 1,823,911 + RR42_RS28835 0.87 -3.7 1,823,995 - -1.5 1,824,059 + -2.7 1,824,059 + -1.5 1,824,229 + -1.0 1,824,508 + RR42_RS28840 0.76 -2.4 1,824,508 + RR42_RS28840 0.76 -2.5 1,824,508 + RR42_RS28840 0.76 -3.0 1,824,508 + RR42_RS28840 0.76 -1.5 1,824,509 - RR42_RS28840 0.76 +0.3 1,824,596 + -2.1 1,824,636 + -1.6 1,824,636 + -2.4 1,824,636 + -2.8 1,824,637 - +0.1 1,824,637 - +0.3 1,824,637 - -1.8 1,824,637 - +0.5 1,824,637 - -1.2 1,824,637 - +0.9 1,824,644 + -1.1 1,824,645 - +0.8 1,824,848 + RR42_RS28845 0.14 -4.7 1,825,092 - RR42_RS28845 0.30 +0.2 1,825,092 - RR42_RS28845 0.30 -0.0 1,825,092 - RR42_RS28845 0.30 -0.4 1,825,268 + RR42_RS28845 0.42 -0.7 1,825,268 + RR42_RS28845 0.42 -1.3 1,825,275 - RR42_RS28845 0.42 -0.2 1,825,371 + RR42_RS28845 0.49 -2.4 1,825,371 + RR42_RS28845 0.49 -2.7 1,825,371 + RR42_RS28845 0.49 -3.5 1,825,371 + RR42_RS28845 0.49 -2.5 1,825,372 - RR42_RS28845 0.49 -1.7 1,825,547 + RR42_RS28845 0.61 -2.0 1,825,736 + -1.1 1,825,737 - RR42_RS28845 0.73 -1.1 1,826,534 - RR42_RS28850 0.44 -0.2 1,826,620 + RR42_RS28850 0.54 -1.7 1,826,621 - RR42_RS28850 0.54 -0.9 1,826,663 + RR42_RS28850 0.59 -0.5 1,827,109 + -0.6
Or see this region's nucleotide sequence