Experiment: D-Gluconic Acid (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00955 and RR42_RS00960 are separated by 79 nucleotides RR42_RS00960 and RR42_RS00965 are separated by 60 nucleotides RR42_RS00965 and RR42_RS00970 are separated by 12 nucleotides RR42_RS00970 and RR42_RS00975 are separated by 22 nucleotides
RR42_RS00955: RR42_RS00955 - hydroxymethylglutaryl-CoA lyase, at 227,673 to 228,608
_RS00955
RR42_RS00960: RR42_RS00960 - prolyl-tRNA synthetase, at 228,688 to 229,167
_RS00960
RR42_RS00965: RR42_RS00965 - Fe-S oxidoreductase, at 229,228 to 229,497
_RS00965
RR42_RS00970: RR42_RS00970 - DSBA oxidoreductase, at 229,510 to 230,199
_RS00970
RR42_RS00975: RR42_RS00975 - MBL fold metallo-hydrolase, at 230,222 to 231,154
_RS00975
Position (kb) 229
230
231 Strain fitness (log2 ratio) -1
0
1 at 228.604 kb on + strand at 228.604 kb on + strand at 228.604 kb on + strand at 228.605 kb on - strand at 228.606 kb on + strand at 228.606 kb on + strand at 228.607 kb on - strand at 228.607 kb on - strand at 228.634 kb on - strand at 228.634 kb on - strand at 228.667 kb on - strand at 228.996 kb on + strand, within RR42_RS00960 at 228.996 kb on + strand, within RR42_RS00960 at 229.181 kb on - strand at 229.181 kb on - strand at 229.181 kb on - strand at 229.345 kb on + strand, within RR42_RS00965 at 229.495 kb on + strand at 229.495 kb on + strand at 229.495 kb on + strand at 229.495 kb on + strand at 229.496 kb on - strand at 229.496 kb on - strand at 229.496 kb on - strand at 229.496 kb on - strand at 229.496 kb on - strand at 229.496 kb on - strand at 229.531 kb on + strand at 229.532 kb on - strand at 229.558 kb on + strand at 229.559 kb on - strand at 229.612 kb on + strand, within RR42_RS00970 at 229.613 kb on - strand, within RR42_RS00970 at 229.613 kb on - strand, within RR42_RS00970 at 229.920 kb on - strand, within RR42_RS00970 at 230.006 kb on + strand, within RR42_RS00970 at 230.006 kb on + strand, within RR42_RS00970 at 230.007 kb on - strand, within RR42_RS00970 at 230.011 kb on - strand, within RR42_RS00970 at 230.011 kb on - strand, within RR42_RS00970 at 230.011 kb on - strand, within RR42_RS00970 at 230.213 kb on + strand at 230.213 kb on + strand at 230.213 kb on + strand at 230.214 kb on - strand at 230.234 kb on + strand at 230.234 kb on + strand at 230.234 kb on + strand at 230.234 kb on + strand at 230.234 kb on + strand at 230.235 kb on - strand at 230.235 kb on - strand at 230.235 kb on - strand at 230.235 kb on - strand at 230.345 kb on + strand, within RR42_RS00975 at 230.547 kb on - strand, within RR42_RS00975 at 230.547 kb on - strand, within RR42_RS00975 at 230.900 kb on + strand, within RR42_RS00975 at 230.900 kb on + strand, within RR42_RS00975 at 230.901 kb on - strand, within RR42_RS00975 at 231.141 kb on - strand at 231.141 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Gluconic Acid (C) remove 228,604 + +0.5 228,604 + +0.3 228,604 + -0.3 228,605 - +0.3 228,606 + +0.9 228,606 + +0.1 228,607 - +1.4 228,607 - -0.5 228,634 - +0.0 228,634 - +0.6 228,667 - +0.0 228,996 + RR42_RS00960 0.64 +0.8 228,996 + RR42_RS00960 0.64 +0.4 229,181 - +0.3 229,181 - +0.8 229,181 - +0.3 229,345 + RR42_RS00965 0.43 +1.2 229,495 + +1.3 229,495 + +0.6 229,495 + +0.9 229,495 + +1.2 229,496 - -0.0 229,496 - -0.4 229,496 - +0.4 229,496 - -0.7 229,496 - -1.0 229,496 - +0.0 229,531 + -0.3 229,532 - -0.3 229,558 + +1.0 229,559 - +0.0 229,612 + RR42_RS00970 0.15 +0.2 229,613 - RR42_RS00970 0.15 +0.6 229,613 - RR42_RS00970 0.15 +0.3 229,920 - RR42_RS00970 0.59 +0.1 230,006 + RR42_RS00970 0.72 +1.2 230,006 + RR42_RS00970 0.72 +1.2 230,007 - RR42_RS00970 0.72 +0.3 230,011 - RR42_RS00970 0.73 -0.2 230,011 - RR42_RS00970 0.73 -0.8 230,011 - RR42_RS00970 0.73 -0.6 230,213 + +0.1 230,213 + +0.0 230,213 + +1.7 230,214 - +0.0 230,234 + -0.3 230,234 + -0.4 230,234 + +0.1 230,234 + +0.1 230,234 + +0.6 230,235 - +0.6 230,235 - -0.4 230,235 - -0.1 230,235 - -0.2 230,345 + RR42_RS00975 0.13 -0.1 230,547 - RR42_RS00975 0.35 +0.7 230,547 - RR42_RS00975 0.35 -0.0 230,900 + RR42_RS00975 0.73 +0.8 230,900 + RR42_RS00975 0.73 +1.3 230,901 - RR42_RS00975 0.73 +1.0 231,141 - -0.1 231,141 - +0.2
Or see this region's nucleotide sequence