Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS07380 and MMJJ_RS07385 are separated by 163 nucleotides MMJJ_RS07385 and MMJJ_RS07390 are separated by 6 nucleotides MMJJ_RS07390 and MMJJ_RS07395 are separated by 16 nucleotides MMJJ_RS07395 and MMJJ_RS07400 are separated by 27 nucleotides
MMJJ_RS07380: MMJJ_RS07380 - sulfide-dependent adenosine diphosphate thiazole synthase, at 1,355,556 to 1,356,344
_RS07380
MMJJ_RS07385: MMJJ_RS07385 - ribosomal protein S18-alanine N-acetyltransferase, at 1,356,508 to 1,356,993
_RS07385
MMJJ_RS07390: MMJJ_RS07390 - TIGR04014 family B12-binding domain/radical SAM domain-containing protein, at 1,357,000 to 1,358,313
_RS07390
MMJJ_RS07395: MMJJ_RS07395 - NAD+ synthase, at 1,358,330 to 1,359,106
_RS07395
MMJJ_RS07400: MMJJ_RS07400 - 2,5-diamino-6-(ribosylamino)-4(3H)-pyrimidinone 5'-phosphate reductase, at 1,359,134 to 1,360,216
_RS07400
Position (kb)
1356
1357
1358
1359 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1356.035 kb on + strand, within MMJJ_RS07380 at 1356.049 kb on - strand, within MMJJ_RS07380 at 1356.049 kb on - strand, within MMJJ_RS07380 at 1356.061 kb on + strand, within MMJJ_RS07380 at 1356.064 kb on - strand, within MMJJ_RS07380 at 1356.065 kb on + strand, within MMJJ_RS07380 at 1356.067 kb on - strand, within MMJJ_RS07380 at 1356.102 kb on + strand, within MMJJ_RS07380 at 1356.120 kb on + strand, within MMJJ_RS07380 at 1356.188 kb on - strand, within MMJJ_RS07380 at 1356.188 kb on - strand, within MMJJ_RS07380 at 1356.205 kb on - strand, within MMJJ_RS07380 at 1356.206 kb on + strand, within MMJJ_RS07380 at 1356.219 kb on - strand, within MMJJ_RS07380 at 1356.219 kb on - strand, within MMJJ_RS07380 at 1356.220 kb on + strand, within MMJJ_RS07380 at 1356.220 kb on + strand, within MMJJ_RS07380 at 1356.246 kb on - strand, within MMJJ_RS07380 at 1356.288 kb on - strand at 1356.338 kb on - strand at 1356.338 kb on - strand at 1356.339 kb on + strand at 1356.342 kb on - strand at 1356.354 kb on + strand at 1356.354 kb on + strand at 1356.354 kb on + strand at 1356.354 kb on + strand at 1356.367 kb on - strand at 1356.376 kb on + strand at 1356.404 kb on - strand at 1356.455 kb on + strand at 1356.455 kb on + strand at 1356.458 kb on - strand at 1356.458 kb on - strand at 1356.458 kb on - strand at 1356.459 kb on + strand at 1356.460 kb on - strand at 1356.460 kb on - strand at 1356.463 kb on - strand at 1356.463 kb on - strand at 1356.463 kb on - strand at 1356.463 kb on - strand at 1356.464 kb on + strand at 1356.468 kb on + strand at 1356.506 kb on - strand at 1356.506 kb on - strand at 1356.506 kb on - strand at 1356.507 kb on + strand at 1356.515 kb on - strand at 1356.519 kb on - strand at 1356.614 kb on - strand, within MMJJ_RS07385 at 1356.614 kb on - strand, within MMJJ_RS07385 at 1356.614 kb on - strand, within MMJJ_RS07385 at 1356.614 kb on - strand, within MMJJ_RS07385 at 1356.614 kb on - strand, within MMJJ_RS07385 at 1356.614 kb on - strand, within MMJJ_RS07385 at 1356.614 kb on - strand, within MMJJ_RS07385 at 1356.615 kb on + strand, within MMJJ_RS07385 at 1356.615 kb on + strand, within MMJJ_RS07385 at 1356.615 kb on + strand, within MMJJ_RS07385 at 1356.616 kb on - strand, within MMJJ_RS07385 at 1356.617 kb on + strand, within MMJJ_RS07385 at 1356.617 kb on + strand, within MMJJ_RS07385 at 1356.617 kb on + strand, within MMJJ_RS07385 at 1356.617 kb on + strand, within MMJJ_RS07385 at 1356.641 kb on - strand, within MMJJ_RS07385 at 1356.677 kb on + strand, within MMJJ_RS07385 at 1356.717 kb on + strand, within MMJJ_RS07385 at 1356.735 kb on + strand, within MMJJ_RS07385 at 1356.735 kb on + strand, within MMJJ_RS07385 at 1356.748 kb on - strand, within MMJJ_RS07385 at 1356.749 kb on + strand, within MMJJ_RS07385 at 1356.771 kb on - strand, within MMJJ_RS07385 at 1356.771 kb on - strand, within MMJJ_RS07385 at 1356.772 kb on + strand, within MMJJ_RS07385 at 1356.866 kb on + strand, within MMJJ_RS07385 at 1356.867 kb on - strand, within MMJJ_RS07385 at 1356.867 kb on - strand, within MMJJ_RS07385 at 1356.868 kb on + strand, within MMJJ_RS07385 at 1356.868 kb on + strand, within MMJJ_RS07385 at 1356.878 kb on + strand, within MMJJ_RS07385 at 1356.885 kb on - strand, within MMJJ_RS07385 at 1356.900 kb on - strand, within MMJJ_RS07385 at 1356.901 kb on + strand, within MMJJ_RS07385 at 1356.901 kb on + strand, within MMJJ_RS07385 at 1356.901 kb on + strand, within MMJJ_RS07385 at 1356.904 kb on - strand, within MMJJ_RS07385 at 1356.905 kb on + strand, within MMJJ_RS07385 at 1356.905 kb on + strand, within MMJJ_RS07385 at 1356.907 kb on - strand, within MMJJ_RS07385 at 1356.930 kb on + strand, within MMJJ_RS07385 at 1356.938 kb on - strand, within MMJJ_RS07385 at 1356.938 kb on - strand, within MMJJ_RS07385 at 1356.968 kb on + strand at 1356.991 kb on - strand at 1356.991 kb on - strand at 1356.991 kb on - strand at 1356.991 kb on - strand at 1356.992 kb on + strand at 1356.996 kb on + strand at 1357.028 kb on - strand at 1357.028 kb on - strand at 1357.028 kb on - strand at 1357.028 kb on - strand at 1357.028 kb on - strand at 1357.028 kb on - strand at 1357.028 kb on - strand at 1357.028 kb on - strand at 1357.028 kb on - strand at 1357.028 kb on - strand at 1357.028 kb on - strand at 1357.029 kb on + strand at 1357.029 kb on + strand at 1357.029 kb on + strand at 1357.029 kb on + strand at 1357.029 kb on + strand at 1357.029 kb on + strand at 1357.030 kb on - strand at 1357.030 kb on - strand at 1357.030 kb on - strand at 1357.030 kb on - strand at 1357.030 kb on - strand at 1357.030 kb on - strand at 1357.030 kb on - strand at 1357.031 kb on + strand at 1357.031 kb on + strand at 1357.031 kb on + strand at 1357.037 kb on + strand at 1357.037 kb on + strand at 1357.083 kb on - strand at 1357.084 kb on + strand at 1357.084 kb on + strand at 1357.084 kb on + strand at 1357.084 kb on + strand at 1357.084 kb on + strand at 1357.086 kb on - strand at 1357.139 kb on - strand, within MMJJ_RS07390 at 1357.139 kb on - strand, within MMJJ_RS07390 at 1357.139 kb on - strand, within MMJJ_RS07390 at 1357.140 kb on + strand, within MMJJ_RS07390 at 1357.143 kb on - strand, within MMJJ_RS07390 at 1357.144 kb on + strand, within MMJJ_RS07390 at 1357.166 kb on - strand, within MMJJ_RS07390 at 1357.209 kb on + strand, within MMJJ_RS07390 at 1357.271 kb on - strand, within MMJJ_RS07390 at 1357.271 kb on - strand, within MMJJ_RS07390 at 1357.317 kb on - strand, within MMJJ_RS07390 at 1357.318 kb on + strand, within MMJJ_RS07390 at 1357.386 kb on - strand, within MMJJ_RS07390 at 1357.387 kb on + strand, within MMJJ_RS07390 at 1357.390 kb on + strand, within MMJJ_RS07390 at 1357.460 kb on - strand, within MMJJ_RS07390 at 1357.475 kb on - strand, within MMJJ_RS07390 at 1357.475 kb on - strand, within MMJJ_RS07390 at 1357.475 kb on - strand, within MMJJ_RS07390 at 1357.475 kb on - strand, within MMJJ_RS07390 at 1357.476 kb on + strand, within MMJJ_RS07390 at 1357.476 kb on + strand, within MMJJ_RS07390 at 1357.476 kb on + strand, within MMJJ_RS07390 at 1357.476 kb on + strand, within MMJJ_RS07390 at 1357.476 kb on + strand, within MMJJ_RS07390 at 1357.477 kb on - strand, within MMJJ_RS07390 at 1357.477 kb on - strand, within MMJJ_RS07390 at 1357.478 kb on + strand, within MMJJ_RS07390 at 1357.478 kb on + strand, within MMJJ_RS07390 at 1357.478 kb on + strand, within MMJJ_RS07390 at 1357.478 kb on + strand, within MMJJ_RS07390 at 1357.479 kb on - strand, within MMJJ_RS07390 at 1357.574 kb on - strand, within MMJJ_RS07390 at 1357.648 kb on - strand, within MMJJ_RS07390 at 1357.710 kb on - strand, within MMJJ_RS07390 at 1357.711 kb on + strand, within MMJJ_RS07390 at 1357.712 kb on - strand, within MMJJ_RS07390 at 1357.712 kb on - strand, within MMJJ_RS07390 at 1357.712 kb on - strand, within MMJJ_RS07390 at 1357.712 kb on - strand, within MMJJ_RS07390 at 1357.713 kb on + strand, within MMJJ_RS07390 at 1357.713 kb on + strand, within MMJJ_RS07390 at 1357.713 kb on + strand, within MMJJ_RS07390 at 1357.713 kb on + strand, within MMJJ_RS07390 at 1357.713 kb on + strand, within MMJJ_RS07390 at 1357.756 kb on - strand, within MMJJ_RS07390 at 1357.792 kb on - strand, within MMJJ_RS07390 at 1357.917 kb on + strand, within MMJJ_RS07390 at 1357.940 kb on - strand, within MMJJ_RS07390 at 1357.941 kb on + strand, within MMJJ_RS07390 at 1357.941 kb on + strand, within MMJJ_RS07390 at 1357.990 kb on + strand, within MMJJ_RS07390 at 1358.022 kb on + strand, within MMJJ_RS07390 at 1358.022 kb on + strand, within MMJJ_RS07390 at 1358.069 kb on - strand, within MMJJ_RS07390 at 1358.069 kb on - strand, within MMJJ_RS07390 at 1358.101 kb on + strand, within MMJJ_RS07390 at 1358.206 kb on + strand at 1358.206 kb on + strand at 1358.208 kb on - strand at 1358.209 kb on + strand at 1358.214 kb on - strand at 1358.214 kb on - strand at 1358.215 kb on + strand at 1358.228 kb on - strand at 1358.228 kb on - strand at 1358.228 kb on - strand at 1358.229 kb on + strand at 1358.229 kb on + strand at 1358.229 kb on + strand at 1358.230 kb on - strand at 1358.262 kb on - strand at 1358.264 kb on - strand at 1358.265 kb on + strand at 1358.265 kb on + strand at 1358.265 kb on + strand at 1358.282 kb on - strand at 1358.286 kb on + strand at 1358.289 kb on - strand at 1358.289 kb on - strand at 1358.289 kb on - strand at 1358.289 kb on - strand at 1358.289 kb on - strand at 1358.290 kb on + strand at 1358.293 kb on - strand at 1358.311 kb on - strand at 1358.372 kb on + strand at 1358.425 kb on + strand, within MMJJ_RS07395 at 1358.861 kb on - strand, within MMJJ_RS07395 at 1359.114 kb on - strand at 1359.168 kb on - strand at 1359.237 kb on - strand at 1359.237 kb on - strand at 1359.238 kb on + strand at 1359.241 kb on + strand at 1359.261 kb on - strand, within MMJJ_RS07400 at 1359.269 kb on + strand, within MMJJ_RS07400 at 1359.306 kb on - strand, within MMJJ_RS07400
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE remove 1,356,035 + MMJJ_RS07380 0.61 -1.2 1,356,049 - MMJJ_RS07380 0.62 -2.5 1,356,049 - MMJJ_RS07380 0.62 -3.1 1,356,061 + MMJJ_RS07380 0.64 -0.5 1,356,064 - MMJJ_RS07380 0.64 -2.1 1,356,065 + MMJJ_RS07380 0.65 -2.0 1,356,067 - MMJJ_RS07380 0.65 -3.1 1,356,102 + MMJJ_RS07380 0.69 -4.1 1,356,120 + MMJJ_RS07380 0.71 -1.8 1,356,188 - MMJJ_RS07380 0.80 -2.6 1,356,188 - MMJJ_RS07380 0.80 -0.8 1,356,205 - MMJJ_RS07380 0.82 -2.2 1,356,206 + MMJJ_RS07380 0.82 -2.7 1,356,219 - MMJJ_RS07380 0.84 -3.4 1,356,219 - MMJJ_RS07380 0.84 -0.2 1,356,220 + MMJJ_RS07380 0.84 -1.7 1,356,220 + MMJJ_RS07380 0.84 -3.4 1,356,246 - MMJJ_RS07380 0.87 -3.8 1,356,288 - -0.2 1,356,338 - -2.4 1,356,338 - -1.9 1,356,339 + -3.2 1,356,342 - +0.7 1,356,354 + -4.0 1,356,354 + -0.5 1,356,354 + -2.1 1,356,354 + -4.4 1,356,367 - -3.0 1,356,376 + +0.6 1,356,404 - +0.1 1,356,455 + +2.0 1,356,455 + +0.4 1,356,458 - -0.3 1,356,458 - -1.8 1,356,458 - +0.9 1,356,459 + +0.0 1,356,460 - +0.8 1,356,460 - +1.6 1,356,463 - -0.9 1,356,463 - +0.0 1,356,463 - +0.5 1,356,463 - -1.8 1,356,464 + +0.5 1,356,468 + -0.5 1,356,506 - -0.4 1,356,506 - +0.2 1,356,506 - +0.4 1,356,507 + +2.2 1,356,515 - -0.2 1,356,519 - -0.2 1,356,614 - MMJJ_RS07385 0.22 -0.2 1,356,614 - MMJJ_RS07385 0.22 +0.1 1,356,614 - MMJJ_RS07385 0.22 +2.3 1,356,614 - MMJJ_RS07385 0.22 +1.0 1,356,614 - MMJJ_RS07385 0.22 +0.2 1,356,614 - MMJJ_RS07385 0.22 -0.4 1,356,614 - MMJJ_RS07385 0.22 +0.1 1,356,615 + MMJJ_RS07385 0.22 -0.7 1,356,615 + MMJJ_RS07385 0.22 +0.7 1,356,615 + MMJJ_RS07385 0.22 -0.1 1,356,616 - MMJJ_RS07385 0.22 +0.6 1,356,617 + MMJJ_RS07385 0.22 +0.5 1,356,617 + MMJJ_RS07385 0.22 +1.6 1,356,617 + MMJJ_RS07385 0.22 +0.3 1,356,617 + MMJJ_RS07385 0.22 +0.6 1,356,641 - MMJJ_RS07385 0.27 +0.7 1,356,677 + MMJJ_RS07385 0.35 +0.7 1,356,717 + MMJJ_RS07385 0.43 +1.7 1,356,735 + MMJJ_RS07385 0.47 +0.7 1,356,735 + MMJJ_RS07385 0.47 +0.8 1,356,748 - MMJJ_RS07385 0.49 -0.2 1,356,749 + MMJJ_RS07385 0.50 +1.9 1,356,771 - MMJJ_RS07385 0.54 -0.4 1,356,771 - MMJJ_RS07385 0.54 +0.5 1,356,772 + MMJJ_RS07385 0.54 +0.9 1,356,866 + MMJJ_RS07385 0.74 +0.4 1,356,867 - MMJJ_RS07385 0.74 +0.6 1,356,867 - MMJJ_RS07385 0.74 +0.8 1,356,868 + MMJJ_RS07385 0.74 +2.6 1,356,868 + MMJJ_RS07385 0.74 -0.8 1,356,878 + MMJJ_RS07385 0.76 +0.4 1,356,885 - MMJJ_RS07385 0.78 -1.3 1,356,900 - MMJJ_RS07385 0.81 +0.1 1,356,901 + MMJJ_RS07385 0.81 -0.1 1,356,901 + MMJJ_RS07385 0.81 -0.7 1,356,901 + MMJJ_RS07385 0.81 +0.2 1,356,904 - MMJJ_RS07385 0.81 +1.4 1,356,905 + MMJJ_RS07385 0.82 +0.7 1,356,905 + MMJJ_RS07385 0.82 +0.4 1,356,907 - MMJJ_RS07385 0.82 -0.5 1,356,930 + MMJJ_RS07385 0.87 +1.1 1,356,938 - MMJJ_RS07385 0.88 +0.1 1,356,938 - MMJJ_RS07385 0.88 +0.4 1,356,968 + +1.1 1,356,991 - -0.4 1,356,991 - +0.2 1,356,991 - +0.4 1,356,991 - -2.7 1,356,992 + +0.3 1,356,996 + +0.9 1,357,028 - +1.7 1,357,028 - +0.2 1,357,028 - -0.2 1,357,028 - +1.5 1,357,028 - -0.3 1,357,028 - -0.8 1,357,028 - +0.5 1,357,028 - -0.2 1,357,028 - -1.7 1,357,028 - -0.2 1,357,028 - -1.2 1,357,029 + +0.8 1,357,029 + -0.6 1,357,029 + +0.8 1,357,029 + -0.0 1,357,029 + -0.2 1,357,029 + +1.7 1,357,030 - +0.3 1,357,030 - +0.1 1,357,030 - +1.1 1,357,030 - +0.9 1,357,030 - +1.0 1,357,030 - +0.5 1,357,030 - -0.2 1,357,031 + -0.2 1,357,031 + +1.0 1,357,031 + -0.4 1,357,037 + +0.2 1,357,037 + -0.5 1,357,083 - +0.3 1,357,084 + -0.1 1,357,084 + +0.0 1,357,084 + -0.6 1,357,084 + -0.1 1,357,084 + +0.1 1,357,086 - +1.8 1,357,139 - MMJJ_RS07390 0.11 +0.8 1,357,139 - MMJJ_RS07390 0.11 -2.0 1,357,139 - MMJJ_RS07390 0.11 +0.2 1,357,140 + MMJJ_RS07390 0.11 -0.0 1,357,143 - MMJJ_RS07390 0.11 -0.9 1,357,144 + MMJJ_RS07390 0.11 -1.2 1,357,166 - MMJJ_RS07390 0.13 +0.1 1,357,209 + MMJJ_RS07390 0.16 +2.0 1,357,271 - MMJJ_RS07390 0.21 -0.1 1,357,271 - MMJJ_RS07390 0.21 +1.7 1,357,317 - MMJJ_RS07390 0.24 +2.1 1,357,318 + MMJJ_RS07390 0.24 -0.5 1,357,386 - MMJJ_RS07390 0.29 -0.6 1,357,387 + MMJJ_RS07390 0.29 +0.5 1,357,390 + MMJJ_RS07390 0.30 +1.1 1,357,460 - MMJJ_RS07390 0.35 +0.7 1,357,475 - MMJJ_RS07390 0.36 +0.2 1,357,475 - MMJJ_RS07390 0.36 -0.7 1,357,475 - MMJJ_RS07390 0.36 +1.5 1,357,475 - MMJJ_RS07390 0.36 +0.9 1,357,476 + MMJJ_RS07390 0.36 +1.4 1,357,476 + MMJJ_RS07390 0.36 +0.9 1,357,476 + MMJJ_RS07390 0.36 +0.6 1,357,476 + MMJJ_RS07390 0.36 +0.4 1,357,476 + MMJJ_RS07390 0.36 -1.1 1,357,477 - MMJJ_RS07390 0.36 +0.0 1,357,477 - MMJJ_RS07390 0.36 +0.1 1,357,478 + MMJJ_RS07390 0.36 +1.3 1,357,478 + MMJJ_RS07390 0.36 +0.2 1,357,478 + MMJJ_RS07390 0.36 +1.8 1,357,478 + MMJJ_RS07390 0.36 -0.4 1,357,479 - MMJJ_RS07390 0.36 +0.2 1,357,574 - MMJJ_RS07390 0.44 -0.2 1,357,648 - MMJJ_RS07390 0.49 -0.3 1,357,710 - MMJJ_RS07390 0.54 +0.8 1,357,711 + MMJJ_RS07390 0.54 +0.4 1,357,712 - MMJJ_RS07390 0.54 +1.2 1,357,712 - MMJJ_RS07390 0.54 +0.3 1,357,712 - MMJJ_RS07390 0.54 +0.0 1,357,712 - MMJJ_RS07390 0.54 +0.4 1,357,713 + MMJJ_RS07390 0.54 -1.7 1,357,713 + MMJJ_RS07390 0.54 +0.7 1,357,713 + MMJJ_RS07390 0.54 +2.6 1,357,713 + MMJJ_RS07390 0.54 +1.2 1,357,713 + MMJJ_RS07390 0.54 +0.7 1,357,756 - MMJJ_RS07390 0.58 -1.3 1,357,792 - MMJJ_RS07390 0.60 +0.5 1,357,917 + MMJJ_RS07390 0.70 +1.1 1,357,940 - MMJJ_RS07390 0.72 -0.5 1,357,941 + MMJJ_RS07390 0.72 -1.1 1,357,941 + MMJJ_RS07390 0.72 -0.3 1,357,990 + MMJJ_RS07390 0.75 +0.2 1,358,022 + MMJJ_RS07390 0.78 +1.2 1,358,022 + MMJJ_RS07390 0.78 +1.2 1,358,069 - MMJJ_RS07390 0.81 -0.3 1,358,069 - MMJJ_RS07390 0.81 -0.2 1,358,101 + MMJJ_RS07390 0.84 +0.3 1,358,206 + -0.2 1,358,206 + +1.2 1,358,208 - -1.0 1,358,209 + +0.2 1,358,214 - -0.3 1,358,214 - -0.0 1,358,215 + +1.4 1,358,228 - +1.0 1,358,228 - -0.4 1,358,228 - -0.3 1,358,229 + +0.4 1,358,229 + +0.5 1,358,229 + +1.6 1,358,230 - +1.5 1,358,262 - -1.7 1,358,264 - -1.5 1,358,265 + +0.1 1,358,265 + +0.6 1,358,265 + -0.4 1,358,282 - -1.3 1,358,286 + +0.6 1,358,289 - +0.5 1,358,289 - +0.1 1,358,289 - -0.2 1,358,289 - -0.8 1,358,289 - -1.0 1,358,290 + +0.1 1,358,293 - +0.1 1,358,311 - -0.2 1,358,372 + -1.0 1,358,425 + MMJJ_RS07395 0.12 -1.0 1,358,861 - MMJJ_RS07395 0.68 +2.2 1,359,114 - +0.4 1,359,168 - -0.0 1,359,237 - +0.7 1,359,237 - +1.3 1,359,238 + +1.8 1,359,241 + -0.2 1,359,261 - MMJJ_RS07400 0.12 +0.3 1,359,269 + MMJJ_RS07400 0.12 +0.7 1,359,306 - MMJJ_RS07400 0.16 +1.2
Or see this region's nucleotide sequence