Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS06590 and MMJJ_RS06595 are separated by 179 nucleotides MMJJ_RS06595 and MMJJ_RS06600 are separated by 132 nucleotides MMJJ_RS06600 and MMJJ_RS06605 are separated by 20 nucleotides
MMJJ_RS06590: MMJJ_RS06590 - sodium:alanine symporter family protein, at 1,217,469 to 1,218,830
_RS06590
MMJJ_RS06595: MMJJ_RS06595 - Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatA, at 1,219,010 to 1,220,305
_RS06595
MMJJ_RS06600: MMJJ_RS06600 - DUF555 domain-containing protein, at 1,220,438 to 1,220,779
_RS06600
MMJJ_RS06605: MMJJ_RS06605 - DUF357 domain-containing protein, at 1,220,800 to 1,221,105
_RS06605
Position (kb)
1219
1220
1221 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1218.028 kb on - strand, within MMJJ_RS06590 at 1218.028 kb on - strand, within MMJJ_RS06590 at 1218.032 kb on - strand, within MMJJ_RS06590 at 1218.068 kb on - strand, within MMJJ_RS06590 at 1218.069 kb on + strand, within MMJJ_RS06590 at 1218.083 kb on - strand, within MMJJ_RS06590 at 1218.083 kb on - strand, within MMJJ_RS06590 at 1218.084 kb on + strand, within MMJJ_RS06590 at 1218.101 kb on - strand, within MMJJ_RS06590 at 1218.107 kb on - strand, within MMJJ_RS06590 at 1218.139 kb on - strand, within MMJJ_RS06590 at 1218.140 kb on + strand, within MMJJ_RS06590 at 1218.171 kb on + strand, within MMJJ_RS06590 at 1218.182 kb on - strand, within MMJJ_RS06590 at 1218.203 kb on + strand, within MMJJ_RS06590 at 1218.203 kb on + strand, within MMJJ_RS06590 at 1218.244 kb on - strand, within MMJJ_RS06590 at 1218.244 kb on - strand, within MMJJ_RS06590 at 1218.245 kb on + strand, within MMJJ_RS06590 at 1218.403 kb on - strand, within MMJJ_RS06590 at 1218.403 kb on - strand, within MMJJ_RS06590 at 1218.403 kb on - strand, within MMJJ_RS06590 at 1218.403 kb on - strand, within MMJJ_RS06590 at 1218.404 kb on + strand, within MMJJ_RS06590 at 1218.404 kb on + strand, within MMJJ_RS06590 at 1218.408 kb on + strand, within MMJJ_RS06590 at 1218.408 kb on + strand, within MMJJ_RS06590 at 1218.508 kb on - strand, within MMJJ_RS06590 at 1218.547 kb on - strand, within MMJJ_RS06590 at 1218.568 kb on - strand, within MMJJ_RS06590 at 1218.585 kb on - strand, within MMJJ_RS06590 at 1218.629 kb on - strand, within MMJJ_RS06590 at 1218.637 kb on + strand, within MMJJ_RS06590 at 1218.655 kb on - strand, within MMJJ_RS06590 at 1218.655 kb on - strand, within MMJJ_RS06590 at 1218.694 kb on - strand at 1218.695 kb on + strand at 1218.710 kb on - strand at 1218.711 kb on + strand at 1218.769 kb on - strand at 1218.819 kb on + strand at 1218.852 kb on + strand at 1218.879 kb on + strand at 1218.884 kb on + strand at 1218.924 kb on - strand at 1218.925 kb on + strand at 1218.926 kb on - strand at 1218.926 kb on - strand at 1218.927 kb on + strand at 1218.932 kb on - strand at 1218.933 kb on + strand at 1218.940 kb on - strand at 1218.940 kb on - strand at 1218.943 kb on - strand at 1218.943 kb on - strand at 1218.944 kb on + strand at 1219.008 kb on + strand at 1220.139 kb on + strand, within MMJJ_RS06595 at 1220.308 kb on - strand at 1220.308 kb on - strand at 1220.311 kb on - strand at 1220.312 kb on + strand at 1220.312 kb on + strand at 1220.374 kb on - strand at 1220.374 kb on - strand at 1220.375 kb on + strand at 1220.378 kb on + strand at 1220.403 kb on - strand at 1220.480 kb on - strand, within MMJJ_RS06600 at 1220.563 kb on - strand, within MMJJ_RS06600 at 1220.567 kb on - strand, within MMJJ_RS06600 at 1220.675 kb on - strand at 1220.720 kb on - strand, within MMJJ_RS06600 at 1220.720 kb on - strand, within MMJJ_RS06600 at 1220.745 kb on - strand at 1220.746 kb on + strand at 1220.763 kb on + strand at 1220.814 kb on - strand at 1220.836 kb on - strand, within MMJJ_RS06605 at 1220.837 kb on + strand, within MMJJ_RS06605 at 1220.908 kb on - strand, within MMJJ_RS06605 at 1220.908 kb on - strand, within MMJJ_RS06605 at 1220.909 kb on + strand at 1220.912 kb on - strand, within MMJJ_RS06605 at 1220.912 kb on - strand, within MMJJ_RS06605 at 1220.926 kb on - strand, within MMJJ_RS06605 at 1220.926 kb on - strand, within MMJJ_RS06605 at 1220.926 kb on - strand, within MMJJ_RS06605 at 1220.928 kb on - strand, within MMJJ_RS06605 at 1220.929 kb on + strand, within MMJJ_RS06605 at 1220.929 kb on + strand, within MMJJ_RS06605 at 1221.026 kb on + strand, within MMJJ_RS06605 at 1221.034 kb on + strand, within MMJJ_RS06605 at 1221.045 kb on - strand, within MMJJ_RS06605 at 1221.045 kb on - strand, within MMJJ_RS06605 at 1221.045 kb on - strand, within MMJJ_RS06605 at 1221.046 kb on + strand, within MMJJ_RS06605 at 1221.082 kb on + strand at 1221.085 kb on + strand at 1221.085 kb on + strand at 1221.085 kb on + strand at 1221.087 kb on - strand at 1221.087 kb on - strand at 1221.088 kb on + strand at 1221.101 kb on + strand at 1221.107 kb on - strand at 1221.107 kb on - strand at 1221.108 kb on + strand at 1221.144 kb on + strand at 1221.176 kb on + strand at 1221.181 kb on - strand at 1221.181 kb on - strand at 1221.182 kb on + strand at 1221.182 kb on + strand at 1221.182 kb on + strand at 1221.182 kb on + strand at 1221.187 kb on - strand at 1221.187 kb on - strand at 1221.192 kb on + strand at 1221.196 kb on - strand at 1221.196 kb on - strand at 1221.196 kb on - strand at 1221.203 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE remove 1,218,028 - MMJJ_RS06590 0.41 -1.5 1,218,028 - MMJJ_RS06590 0.41 +0.9 1,218,032 - MMJJ_RS06590 0.41 -0.0 1,218,068 - MMJJ_RS06590 0.44 -0.2 1,218,069 + MMJJ_RS06590 0.44 +0.3 1,218,083 - MMJJ_RS06590 0.45 -0.0 1,218,083 - MMJJ_RS06590 0.45 -0.0 1,218,084 + MMJJ_RS06590 0.45 +1.7 1,218,101 - MMJJ_RS06590 0.46 -0.0 1,218,107 - MMJJ_RS06590 0.47 -2.3 1,218,139 - MMJJ_RS06590 0.49 +0.5 1,218,140 + MMJJ_RS06590 0.49 -0.8 1,218,171 + MMJJ_RS06590 0.52 -0.5 1,218,182 - MMJJ_RS06590 0.52 +0.2 1,218,203 + MMJJ_RS06590 0.54 -0.2 1,218,203 + MMJJ_RS06590 0.54 -0.9 1,218,244 - MMJJ_RS06590 0.57 -1.1 1,218,244 - MMJJ_RS06590 0.57 +0.9 1,218,245 + MMJJ_RS06590 0.57 -0.1 1,218,403 - MMJJ_RS06590 0.69 -1.1 1,218,403 - MMJJ_RS06590 0.69 +0.4 1,218,403 - MMJJ_RS06590 0.69 -1.4 1,218,403 - MMJJ_RS06590 0.69 +0.8 1,218,404 + MMJJ_RS06590 0.69 +0.6 1,218,404 + MMJJ_RS06590 0.69 +1.4 1,218,408 + MMJJ_RS06590 0.69 -0.6 1,218,408 + MMJJ_RS06590 0.69 -1.6 1,218,508 - MMJJ_RS06590 0.76 +0.3 1,218,547 - MMJJ_RS06590 0.79 +1.4 1,218,568 - MMJJ_RS06590 0.81 +0.9 1,218,585 - MMJJ_RS06590 0.82 -0.6 1,218,629 - MMJJ_RS06590 0.85 +0.5 1,218,637 + MMJJ_RS06590 0.86 -0.0 1,218,655 - MMJJ_RS06590 0.87 +1.0 1,218,655 - MMJJ_RS06590 0.87 +1.5 1,218,694 - +0.3 1,218,695 + +0.1 1,218,710 - +0.9 1,218,711 + -1.3 1,218,769 - -2.2 1,218,819 + +1.0 1,218,852 + +1.5 1,218,879 + -0.6 1,218,884 + +0.1 1,218,924 - +0.2 1,218,925 + +0.2 1,218,926 - +1.9 1,218,926 - +0.4 1,218,927 + +0.8 1,218,932 - -0.1 1,218,933 + +1.3 1,218,940 - +0.4 1,218,940 - -0.2 1,218,943 - -0.1 1,218,943 - +0.7 1,218,944 + +1.4 1,219,008 + -0.3 1,220,139 + MMJJ_RS06595 0.87 -1.7 1,220,308 - +1.0 1,220,308 - -0.4 1,220,311 - +0.8 1,220,312 + +1.4 1,220,312 + -0.4 1,220,374 - +0.4 1,220,374 - +0.9 1,220,375 + +0.6 1,220,378 + -0.3 1,220,403 - +0.9 1,220,480 - MMJJ_RS06600 0.12 -1.2 1,220,563 - MMJJ_RS06600 0.37 -2.9 1,220,567 - MMJJ_RS06600 0.38 -1.2 1,220,675 - +1.0 1,220,720 - MMJJ_RS06600 0.82 -2.4 1,220,720 - MMJJ_RS06600 0.82 -2.3 1,220,745 - -2.6 1,220,746 + +1.0 1,220,763 + -1.2 1,220,814 - -3.1 1,220,836 - MMJJ_RS06605 0.12 -0.6 1,220,837 + MMJJ_RS06605 0.12 -2.7 1,220,908 - MMJJ_RS06605 0.35 -2.2 1,220,908 - MMJJ_RS06605 0.35 -3.9 1,220,909 + -0.3 1,220,912 - MMJJ_RS06605 0.37 -2.2 1,220,912 - MMJJ_RS06605 0.37 -1.3 1,220,926 - MMJJ_RS06605 0.41 -3.2 1,220,926 - MMJJ_RS06605 0.41 -1.6 1,220,926 - MMJJ_RS06605 0.41 -2.4 1,220,928 - MMJJ_RS06605 0.42 -1.6 1,220,929 + MMJJ_RS06605 0.42 -2.4 1,220,929 + MMJJ_RS06605 0.42 -2.1 1,221,026 + MMJJ_RS06605 0.74 +0.2 1,221,034 + MMJJ_RS06605 0.76 -1.2 1,221,045 - MMJJ_RS06605 0.80 +0.3 1,221,045 - MMJJ_RS06605 0.80 +1.4 1,221,045 - MMJJ_RS06605 0.80 -0.3 1,221,046 + MMJJ_RS06605 0.80 -1.6 1,221,082 + +1.0 1,221,085 + -0.9 1,221,085 + -0.6 1,221,085 + -0.4 1,221,087 - -1.5 1,221,087 - +0.3 1,221,088 + -1.4 1,221,101 + -1.0 1,221,107 - +0.7 1,221,107 - -0.4 1,221,108 + -0.6 1,221,144 + +0.3 1,221,176 + +0.8 1,221,181 - +0.9 1,221,181 - -0.6 1,221,182 + +2.0 1,221,182 + -0.2 1,221,182 + +0.2 1,221,182 + +0.4 1,221,187 - +0.3 1,221,187 - +0.9 1,221,192 + +2.1 1,221,196 - +1.0 1,221,196 - -0.8 1,221,196 - -0.0 1,221,203 + +2.1
Or see this region's nucleotide sequence