Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS04930 and MMJJ_RS04935 are separated by 142 nucleotides MMJJ_RS04935 and MMJJ_RS04940 are separated by 30 nucleotides MMJJ_RS04940 and MMJJ_RS04945 are separated by 124 nucleotides
MMJJ_RS04930: MMJJ_RS04930 - MFS transporter, at 916,357 to 917,508
_RS04930
MMJJ_RS04935: MMJJ_RS04935 - 4Fe-4S binding protein, at 917,651 to 917,848
_RS04935
MMJJ_RS04940: MMJJ_RS04940 - XRE family transcriptional regulator, at 917,879 to 918,427
_RS04940
MMJJ_RS04945: MMJJ_RS04945 - PLP-dependent aminotransferase family protein, at 918,552 to 919,739
_RS04945
Position (kb) 917
918 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 916.675 kb on - strand, within MMJJ_RS04930 at 916.738 kb on - strand, within MMJJ_RS04930 at 916.738 kb on - strand, within MMJJ_RS04930 at 916.864 kb on - strand, within MMJJ_RS04930 at 916.865 kb on + strand, within MMJJ_RS04930 at 916.865 kb on + strand, within MMJJ_RS04930 at 916.870 kb on + strand, within MMJJ_RS04930 at 916.959 kb on - strand, within MMJJ_RS04930 at 916.996 kb on - strand, within MMJJ_RS04930 at 916.996 kb on - strand, within MMJJ_RS04930 at 916.996 kb on - strand, within MMJJ_RS04930 at 916.997 kb on + strand, within MMJJ_RS04930 at 917.003 kb on + strand, within MMJJ_RS04930 at 917.015 kb on + strand, within MMJJ_RS04930 at 917.015 kb on + strand at 917.018 kb on - strand, within MMJJ_RS04930 at 917.018 kb on - strand, within MMJJ_RS04930 at 917.019 kb on + strand, within MMJJ_RS04930 at 917.019 kb on + strand, within MMJJ_RS04930 at 917.019 kb on + strand, within MMJJ_RS04930 at 917.039 kb on - strand, within MMJJ_RS04930 at 917.039 kb on - strand, within MMJJ_RS04930 at 917.039 kb on - strand, within MMJJ_RS04930 at 917.039 kb on - strand, within MMJJ_RS04930 at 917.039 kb on - strand, within MMJJ_RS04930 at 917.040 kb on + strand, within MMJJ_RS04930 at 917.043 kb on - strand at 917.044 kb on + strand, within MMJJ_RS04930 at 917.044 kb on + strand, within MMJJ_RS04930 at 917.044 kb on + strand, within MMJJ_RS04930 at 917.053 kb on - strand, within MMJJ_RS04930 at 917.053 kb on - strand, within MMJJ_RS04930 at 917.108 kb on + strand, within MMJJ_RS04930 at 917.117 kb on - strand, within MMJJ_RS04930 at 917.117 kb on - strand, within MMJJ_RS04930 at 917.118 kb on + strand, within MMJJ_RS04930 at 917.118 kb on + strand, within MMJJ_RS04930 at 917.154 kb on - strand, within MMJJ_RS04930 at 917.156 kb on - strand, within MMJJ_RS04930 at 917.208 kb on - strand, within MMJJ_RS04930 at 917.209 kb on + strand, within MMJJ_RS04930 at 917.220 kb on - strand, within MMJJ_RS04930 at 917.221 kb on + strand, within MMJJ_RS04930 at 917.354 kb on + strand, within MMJJ_RS04930 at 917.476 kb on - strand at 917.483 kb on - strand at 917.484 kb on + strand at 917.484 kb on + strand at 917.521 kb on - strand at 917.521 kb on - strand at 917.528 kb on - strand at 917.528 kb on - strand at 917.528 kb on - strand at 917.528 kb on - strand at 917.529 kb on + strand at 917.529 kb on + strand at 917.530 kb on - strand at 917.530 kb on - strand at 917.531 kb on + strand at 917.553 kb on - strand at 917.553 kb on - strand at 917.554 kb on + strand at 917.554 kb on + strand at 917.554 kb on + strand at 917.554 kb on + strand at 917.557 kb on - strand at 917.557 kb on - strand at 917.557 kb on - strand at 917.558 kb on + strand at 917.558 kb on + strand at 917.558 kb on + strand at 917.558 kb on + strand at 917.558 kb on + strand at 917.571 kb on - strand at 917.572 kb on + strand at 917.572 kb on + strand at 917.581 kb on - strand at 917.582 kb on + strand at 917.592 kb on + strand at 917.597 kb on - strand at 917.597 kb on - strand at 917.597 kb on - strand at 917.597 kb on - strand at 917.598 kb on + strand at 917.598 kb on + strand at 917.601 kb on - strand at 917.601 kb on - strand at 917.601 kb on - strand at 917.602 kb on + strand at 917.602 kb on + strand at 917.602 kb on + strand at 917.608 kb on - strand at 917.617 kb on - strand at 917.617 kb on - strand at 917.617 kb on - strand at 917.618 kb on + strand at 917.618 kb on + strand at 917.646 kb on - strand at 917.646 kb on - strand at 917.647 kb on + strand at 917.647 kb on + strand at 917.650 kb on - strand at 917.651 kb on + strand at 917.655 kb on - strand at 917.656 kb on + strand at 917.656 kb on + strand at 917.656 kb on + strand at 917.657 kb on - strand at 917.657 kb on - strand at 917.657 kb on - strand at 917.658 kb on + strand at 917.659 kb on - strand at 917.659 kb on - strand at 917.659 kb on - strand at 917.660 kb on + strand at 917.660 kb on + strand at 917.660 kb on + strand at 917.660 kb on + strand at 917.660 kb on + strand at 917.660 kb on + strand at 917.681 kb on + strand, within MMJJ_RS04935 at 917.715 kb on - strand, within MMJJ_RS04935 at 917.716 kb on + strand, within MMJJ_RS04935 at 917.722 kb on - strand, within MMJJ_RS04935 at 917.744 kb on + strand, within MMJJ_RS04935 at 917.796 kb on - strand, within MMJJ_RS04935 at 917.796 kb on - strand, within MMJJ_RS04935 at 917.797 kb on + strand, within MMJJ_RS04935 at 917.805 kb on - strand, within MMJJ_RS04935 at 917.805 kb on - strand, within MMJJ_RS04935 at 917.847 kb on + strand at 917.856 kb on - strand at 917.856 kb on - strand at 917.865 kb on + strand at 917.865 kb on + strand at 917.870 kb on + strand at 917.870 kb on + strand at 917.896 kb on - strand at 917.897 kb on + strand at 917.989 kb on + strand, within MMJJ_RS04940 at 918.037 kb on - strand, within MMJJ_RS04940 at 918.037 kb on - strand, within MMJJ_RS04940 at 918.072 kb on - strand, within MMJJ_RS04940 at 918.072 kb on - strand, within MMJJ_RS04940 at 918.072 kb on - strand, within MMJJ_RS04940 at 918.072 kb on - strand, within MMJJ_RS04940 at 918.073 kb on + strand, within MMJJ_RS04940 at 918.073 kb on + strand, within MMJJ_RS04940 at 918.073 kb on + strand, within MMJJ_RS04940 at 918.076 kb on + strand, within MMJJ_RS04940 at 918.092 kb on - strand, within MMJJ_RS04940 at 918.103 kb on - strand, within MMJJ_RS04940 at 918.104 kb on + strand, within MMJJ_RS04940 at 918.104 kb on + strand, within MMJJ_RS04940 at 918.105 kb on - strand, within MMJJ_RS04940 at 918.105 kb on - strand, within MMJJ_RS04940 at 918.105 kb on - strand, within MMJJ_RS04940 at 918.105 kb on - strand, within MMJJ_RS04940 at 918.105 kb on - strand, within MMJJ_RS04940 at 918.106 kb on + strand, within MMJJ_RS04940 at 918.106 kb on + strand at 918.141 kb on + strand, within MMJJ_RS04940 at 918.183 kb on + strand, within MMJJ_RS04940 at 918.227 kb on - strand, within MMJJ_RS04940 at 918.228 kb on + strand, within MMJJ_RS04940 at 918.293 kb on - strand, within MMJJ_RS04940 at 918.293 kb on - strand, within MMJJ_RS04940 at 918.328 kb on - strand, within MMJJ_RS04940 at 918.329 kb on + strand, within MMJJ_RS04940 at 918.347 kb on + strand, within MMJJ_RS04940 at 918.366 kb on + strand, within MMJJ_RS04940 at 918.415 kb on - strand at 918.415 kb on - strand at 918.415 kb on - strand at 918.416 kb on + strand at 918.446 kb on + strand at 918.447 kb on - strand at 918.448 kb on + strand at 918.452 kb on - strand at 918.452 kb on - strand at 918.452 kb on - strand at 918.452 kb on - strand at 918.452 kb on - strand at 918.453 kb on + strand at 918.453 kb on + strand at 918.453 kb on + strand at 918.453 kb on + strand at 918.453 kb on + strand at 918.454 kb on - strand at 918.454 kb on - strand at 918.455 kb on + strand at 918.455 kb on + strand at 918.455 kb on + strand at 918.455 kb on + strand at 918.456 kb on - strand at 918.458 kb on - strand at 918.458 kb on - strand at 918.459 kb on + strand at 918.459 kb on + strand at 918.467 kb on - strand at 918.468 kb on + strand at 918.468 kb on + strand at 918.469 kb on - strand at 918.469 kb on - strand at 918.470 kb on + strand at 918.470 kb on + strand at 918.477 kb on - strand at 918.478 kb on + strand at 918.479 kb on - strand at 918.488 kb on - strand at 918.488 kb on - strand at 918.489 kb on + strand at 918.489 kb on + strand at 918.493 kb on - strand at 918.493 kb on - strand at 918.493 kb on - strand at 918.494 kb on + strand at 918.495 kb on - strand at 918.495 kb on - strand at 918.523 kb on - strand at 918.551 kb on - strand at 918.692 kb on + strand, within MMJJ_RS04945 at 918.739 kb on - strand, within MMJJ_RS04945 at 918.800 kb on - strand, within MMJJ_RS04945 at 918.808 kb on - strand, within MMJJ_RS04945 at 918.809 kb on + strand, within MMJJ_RS04945 at 918.822 kb on + strand, within MMJJ_RS04945 at 918.839 kb on + strand, within MMJJ_RS04945
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE remove 916,675 - MMJJ_RS04930 0.28 +0.1 916,738 - MMJJ_RS04930 0.33 -0.2 916,738 - MMJJ_RS04930 0.33 -0.1 916,864 - MMJJ_RS04930 0.44 -0.1 916,865 + MMJJ_RS04930 0.44 -0.4 916,865 + MMJJ_RS04930 0.44 -0.9 916,870 + MMJJ_RS04930 0.45 -0.4 916,959 - MMJJ_RS04930 0.52 +0.0 916,996 - MMJJ_RS04930 0.55 -0.7 916,996 - MMJJ_RS04930 0.55 +0.0 916,996 - MMJJ_RS04930 0.55 -0.8 916,997 + MMJJ_RS04930 0.56 +1.7 917,003 + MMJJ_RS04930 0.56 +1.8 917,015 + MMJJ_RS04930 0.57 -0.5 917,015 + +1.5 917,018 - MMJJ_RS04930 0.57 -0.4 917,018 - MMJJ_RS04930 0.57 -1.8 917,019 + MMJJ_RS04930 0.57 -0.4 917,019 + MMJJ_RS04930 0.57 -0.9 917,019 + MMJJ_RS04930 0.57 -0.6 917,039 - MMJJ_RS04930 0.59 +0.2 917,039 - MMJJ_RS04930 0.59 -1.1 917,039 - MMJJ_RS04930 0.59 +0.3 917,039 - MMJJ_RS04930 0.59 -0.3 917,039 - MMJJ_RS04930 0.59 -1.1 917,040 + MMJJ_RS04930 0.59 +0.2 917,043 - +3.1 917,044 + MMJJ_RS04930 0.60 -1.6 917,044 + MMJJ_RS04930 0.60 +0.7 917,044 + MMJJ_RS04930 0.60 +2.3 917,053 - MMJJ_RS04930 0.60 -0.3 917,053 - MMJJ_RS04930 0.60 -0.3 917,108 + MMJJ_RS04930 0.65 +0.9 917,117 - MMJJ_RS04930 0.66 -1.2 917,117 - MMJJ_RS04930 0.66 +1.6 917,118 + MMJJ_RS04930 0.66 -0.3 917,118 + MMJJ_RS04930 0.66 +1.2 917,154 - MMJJ_RS04930 0.69 -0.4 917,156 - MMJJ_RS04930 0.69 -0.1 917,208 - MMJJ_RS04930 0.74 -0.3 917,209 + MMJJ_RS04930 0.74 +0.2 917,220 - MMJJ_RS04930 0.75 -1.9 917,221 + MMJJ_RS04930 0.75 -0.6 917,354 + MMJJ_RS04930 0.87 +0.8 917,476 - -0.7 917,483 - -1.1 917,484 + +0.0 917,484 + +2.0 917,521 - +0.5 917,521 - -0.6 917,528 - +0.4 917,528 - -0.6 917,528 - +0.3 917,528 - -0.5 917,529 + -1.5 917,529 + +0.1 917,530 - +0.0 917,530 - +0.7 917,531 + -0.1 917,553 - -0.6 917,553 - +0.6 917,554 + -0.6 917,554 + -0.6 917,554 + +1.0 917,554 + -0.0 917,557 - +0.7 917,557 - +1.6 917,557 - +1.3 917,558 + +0.2 917,558 + +0.7 917,558 + +1.0 917,558 + +0.8 917,558 + -0.1 917,571 - -0.1 917,572 + -0.2 917,572 + -0.1 917,581 - +0.6 917,582 + -0.4 917,592 + +0.9 917,597 - -1.7 917,597 - -0.5 917,597 - -1.1 917,597 - -0.7 917,598 + -1.2 917,598 + +0.6 917,601 - -2.1 917,601 - -1.3 917,601 - -1.6 917,602 + -1.7 917,602 + +0.6 917,602 + -0.4 917,608 - -1.1 917,617 - -1.2 917,617 - -0.5 917,617 - -1.6 917,618 + -2.2 917,618 + +0.2 917,646 - +2.1 917,646 - -1.8 917,647 + -1.3 917,647 + -0.9 917,650 - -1.2 917,651 + -0.3 917,655 - -0.8 917,656 + -2.0 917,656 + -1.5 917,656 + -0.9 917,657 - -2.3 917,657 - -2.4 917,657 - -0.9 917,658 + -3.4 917,659 - -2.3 917,659 - -0.9 917,659 - -2.5 917,660 + -1.2 917,660 + -0.9 917,660 + -0.6 917,660 + -0.9 917,660 + -0.3 917,660 + -1.6 917,681 + MMJJ_RS04935 0.15 -0.1 917,715 - MMJJ_RS04935 0.32 -1.4 917,716 + MMJJ_RS04935 0.33 -0.8 917,722 - MMJJ_RS04935 0.36 -1.8 917,744 + MMJJ_RS04935 0.47 -0.9 917,796 - MMJJ_RS04935 0.73 -0.2 917,796 - MMJJ_RS04935 0.73 -0.3 917,797 + MMJJ_RS04935 0.74 -0.8 917,805 - MMJJ_RS04935 0.78 -1.4 917,805 - MMJJ_RS04935 0.78 -0.4 917,847 + +0.1 917,856 - -0.2 917,856 - -0.1 917,865 + +0.2 917,865 + -0.3 917,870 + -0.5 917,870 + +1.9 917,896 - -0.2 917,897 + -1.2 917,989 + MMJJ_RS04940 0.20 -0.2 918,037 - MMJJ_RS04940 0.29 -0.7 918,037 - MMJJ_RS04940 0.29 +0.7 918,072 - MMJJ_RS04940 0.35 +0.8 918,072 - MMJJ_RS04940 0.35 +1.1 918,072 - MMJJ_RS04940 0.35 +0.2 918,072 - MMJJ_RS04940 0.35 -1.5 918,073 + MMJJ_RS04940 0.35 -0.3 918,073 + MMJJ_RS04940 0.35 -1.2 918,073 + MMJJ_RS04940 0.35 +0.5 918,076 + MMJJ_RS04940 0.36 -0.6 918,092 - MMJJ_RS04940 0.39 -1.4 918,103 - MMJJ_RS04940 0.41 +0.6 918,104 + MMJJ_RS04940 0.41 -1.1 918,104 + MMJJ_RS04940 0.41 -0.5 918,105 - MMJJ_RS04940 0.41 -2.0 918,105 - MMJJ_RS04940 0.41 -0.2 918,105 - MMJJ_RS04940 0.41 +0.5 918,105 - MMJJ_RS04940 0.41 -0.3 918,105 - MMJJ_RS04940 0.41 +0.6 918,106 + MMJJ_RS04940 0.41 -0.1 918,106 + -0.1 918,141 + MMJJ_RS04940 0.48 +0.0 918,183 + MMJJ_RS04940 0.55 -0.8 918,227 - MMJJ_RS04940 0.63 +1.0 918,228 + MMJJ_RS04940 0.64 -0.4 918,293 - MMJJ_RS04940 0.75 +1.4 918,293 - MMJJ_RS04940 0.75 +0.4 918,328 - MMJJ_RS04940 0.82 +0.5 918,329 + MMJJ_RS04940 0.82 -0.8 918,347 + MMJJ_RS04940 0.85 -0.7 918,366 + MMJJ_RS04940 0.89 +0.6 918,415 - +0.7 918,415 - -0.4 918,415 - -1.2 918,416 + +0.5 918,446 + -0.9 918,447 - -1.1 918,448 + +0.6 918,452 - -0.3 918,452 - -1.4 918,452 - -0.1 918,452 - -1.9 918,452 - +1.5 918,453 + -0.1 918,453 + +0.9 918,453 + -0.4 918,453 + -0.3 918,453 + -0.8 918,454 - +2.0 918,454 - -0.3 918,455 + -0.3 918,455 + -0.1 918,455 + -0.1 918,455 + -1.8 918,456 - +0.4 918,458 - +1.2 918,458 - +0.8 918,459 + +0.9 918,459 + +0.8 918,467 - +1.0 918,468 + +1.0 918,468 + -0.2 918,469 - -0.2 918,469 - -0.9 918,470 + -1.0 918,470 + +0.9 918,477 - +0.2 918,478 + +0.6 918,479 - +0.3 918,488 - -2.3 918,488 - +0.8 918,489 + +2.2 918,489 + +1.1 918,493 - +0.9 918,493 - -0.1 918,493 - -0.0 918,494 + +0.9 918,495 - +0.9 918,495 - +0.9 918,523 - -0.6 918,551 - -0.0 918,692 + MMJJ_RS04945 0.12 -0.2 918,739 - MMJJ_RS04945 0.16 +0.1 918,800 - MMJJ_RS04945 0.21 +0.3 918,808 - MMJJ_RS04945 0.22 +1.1 918,809 + MMJJ_RS04945 0.22 +0.6 918,822 + MMJJ_RS04945 0.23 -1.6 918,839 + MMJJ_RS04945 0.24 +0.4
Or see this region's nucleotide sequence