Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS01755 and MMJJ_RS01760 are separated by 94 nucleotides MMJJ_RS01760 and MMJJ_RS01765 are separated by 101 nucleotides MMJJ_RS01765 and MMJJ_RS01770 are separated by 167 nucleotides MMJJ_RS01770 and MMJJ_RS01775 are separated by 323 nucleotides MMJJ_RS01775 and MMJJ_RS01780 are separated by 9 nucleotides
MMJJ_RS01755: MMJJ_RS01755 - ferrous iron transport protein B, at 339,231 to 341,174
_RS01755
MMJJ_RS01760: MMJJ_RS01760 - N-acetyltransferase, at 341,269 to 341,877
_RS01760
MMJJ_RS01765: MMJJ_RS01765 - winged helix-turn-helix domain-containing protein, at 341,979 to 342,377
_RS01765
MMJJ_RS01770: MMJJ_RS01770 - TOBE domain-containing protein, at 342,545 to 342,751
_RS01770
MMJJ_RS01775: MMJJ_RS01775 - 50S ribosomal protein L34e, at 343,075 to 343,344
_RS01775
MMJJ_RS01780: MMJJ_RS01780 - AAA family ATPase, at 343,354 to 343,890
_RS01780
Position (kb) 341
342
343 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 341.031 kb on + strand at 341.059 kb on - strand at 341.145 kb on - strand at 341.146 kb on + strand at 341.242 kb on - strand at 341.242 kb on - strand at 341.242 kb on - strand at 341.245 kb on - strand at 341.245 kb on - strand at 341.245 kb on - strand at 341.246 kb on + strand at 341.246 kb on + strand at 341.247 kb on - strand at 341.247 kb on - strand at 341.247 kb on - strand at 341.247 kb on - strand at 341.248 kb on + strand at 341.250 kb on - strand at 341.250 kb on - strand at 341.251 kb on + strand at 341.252 kb on - strand at 341.252 kb on - strand at 341.252 kb on - strand at 341.252 kb on - strand at 341.253 kb on + strand at 341.253 kb on + strand at 341.253 kb on + strand at 341.253 kb on + strand at 341.264 kb on - strand at 341.265 kb on + strand at 341.265 kb on + strand at 341.265 kb on + strand at 341.275 kb on + strand at 341.275 kb on + strand at 341.276 kb on - strand at 341.276 kb on - strand at 341.277 kb on + strand at 341.296 kb on + strand at 341.308 kb on + strand at 341.322 kb on - strand at 341.348 kb on - strand, within MMJJ_RS01760 at 341.349 kb on + strand, within MMJJ_RS01760 at 341.349 kb on + strand, within MMJJ_RS01760 at 341.349 kb on + strand, within MMJJ_RS01760 at 341.349 kb on + strand, within MMJJ_RS01760 at 341.375 kb on - strand, within MMJJ_RS01760 at 341.428 kb on + strand, within MMJJ_RS01760 at 341.461 kb on + strand, within MMJJ_RS01760 at 341.463 kb on - strand, within MMJJ_RS01760 at 341.464 kb on + strand, within MMJJ_RS01760 at 341.467 kb on - strand, within MMJJ_RS01760 at 341.483 kb on - strand, within MMJJ_RS01760 at 341.484 kb on + strand, within MMJJ_RS01760 at 341.484 kb on + strand, within MMJJ_RS01760 at 341.529 kb on - strand, within MMJJ_RS01760 at 341.530 kb on + strand, within MMJJ_RS01760 at 341.544 kb on - strand, within MMJJ_RS01760 at 341.610 kb on + strand, within MMJJ_RS01760 at 341.610 kb on + strand, within MMJJ_RS01760 at 341.647 kb on - strand, within MMJJ_RS01760 at 341.647 kb on - strand, within MMJJ_RS01760 at 341.648 kb on + strand, within MMJJ_RS01760 at 341.648 kb on + strand, within MMJJ_RS01760 at 341.667 kb on - strand, within MMJJ_RS01760 at 341.760 kb on - strand, within MMJJ_RS01760 at 341.777 kb on + strand, within MMJJ_RS01760 at 341.777 kb on + strand, within MMJJ_RS01760 at 341.781 kb on + strand, within MMJJ_RS01760 at 341.795 kb on - strand, within MMJJ_RS01760 at 341.796 kb on + strand, within MMJJ_RS01760 at 341.796 kb on + strand, within MMJJ_RS01760 at 341.800 kb on + strand, within MMJJ_RS01760 at 341.840 kb on + strand at 341.841 kb on - strand at 341.857 kb on + strand at 341.871 kb on + strand at 341.871 kb on + strand at 341.876 kb on + strand at 341.884 kb on - strand at 341.927 kb on - strand at 341.931 kb on - strand at 341.931 kb on - strand at 341.962 kb on + strand at 341.996 kb on - strand at 341.997 kb on + strand at 342.079 kb on + strand, within MMJJ_RS01765 at 342.079 kb on + strand, within MMJJ_RS01765 at 342.080 kb on - strand, within MMJJ_RS01765 at 342.081 kb on + strand, within MMJJ_RS01765 at 342.100 kb on + strand, within MMJJ_RS01765 at 342.103 kb on + strand, within MMJJ_RS01765 at 342.103 kb on + strand, within MMJJ_RS01765 at 342.181 kb on - strand, within MMJJ_RS01765 at 342.182 kb on + strand, within MMJJ_RS01765 at 342.193 kb on - strand, within MMJJ_RS01765 at 342.193 kb on - strand, within MMJJ_RS01765 at 342.194 kb on + strand, within MMJJ_RS01765 at 342.194 kb on + strand, within MMJJ_RS01765 at 342.205 kb on + strand, within MMJJ_RS01765 at 342.219 kb on + strand, within MMJJ_RS01765 at 342.219 kb on + strand, within MMJJ_RS01765 at 342.231 kb on - strand, within MMJJ_RS01765 at 342.232 kb on + strand, within MMJJ_RS01765 at 342.232 kb on + strand, within MMJJ_RS01765 at 342.288 kb on - strand, within MMJJ_RS01765 at 342.288 kb on - strand, within MMJJ_RS01765 at 342.289 kb on + strand, within MMJJ_RS01765 at 342.318 kb on - strand, within MMJJ_RS01765 at 342.318 kb on - strand, within MMJJ_RS01765 at 342.330 kb on - strand, within MMJJ_RS01765 at 342.330 kb on - strand, within MMJJ_RS01765 at 342.330 kb on - strand, within MMJJ_RS01765 at 342.331 kb on + strand, within MMJJ_RS01765 at 342.331 kb on + strand, within MMJJ_RS01765 at 342.371 kb on - strand at 342.371 kb on - strand at 342.372 kb on + strand at 342.377 kb on - strand at 342.378 kb on + strand at 342.406 kb on - strand at 342.407 kb on + strand at 342.430 kb on + strand at 342.430 kb on + strand at 342.433 kb on - strand at 342.433 kb on - strand at 342.434 kb on + strand at 342.434 kb on + strand at 342.435 kb on - strand at 342.436 kb on + strand at 342.436 kb on + strand at 342.437 kb on - strand at 342.438 kb on + strand at 342.438 kb on + strand at 342.438 kb on + strand at 342.438 kb on + strand at 342.439 kb on - strand at 342.440 kb on + strand at 342.443 kb on - strand at 342.443 kb on - strand at 342.459 kb on + strand at 342.460 kb on - strand at 342.463 kb on - strand at 342.464 kb on + strand at 342.464 kb on + strand at 342.476 kb on + strand at 342.477 kb on - strand at 342.477 kb on - strand at 342.477 kb on - strand at 342.478 kb on + strand at 342.478 kb on + strand at 342.478 kb on + strand at 342.478 kb on + strand at 342.478 kb on + strand at 342.479 kb on - strand at 342.480 kb on + strand at 342.480 kb on + strand at 342.499 kb on - strand at 342.587 kb on - strand, within MMJJ_RS01770 at 342.634 kb on - strand, within MMJJ_RS01770 at 342.638 kb on - strand, within MMJJ_RS01770 at 342.653 kb on + strand, within MMJJ_RS01770 at 342.689 kb on - strand, within MMJJ_RS01770 at 342.690 kb on + strand, within MMJJ_RS01770 at 342.753 kb on - strand at 342.753 kb on - strand at 342.753 kb on - strand at 342.754 kb on + strand at 342.754 kb on + strand at 342.754 kb on + strand at 342.754 kb on + strand at 342.754 kb on + strand at 342.754 kb on + strand at 342.754 kb on + strand at 342.776 kb on - strand at 342.780 kb on - strand at 342.780 kb on - strand at 342.785 kb on - strand at 342.785 kb on - strand at 342.788 kb on + strand at 342.800 kb on + strand at 342.807 kb on - strand at 342.807 kb on - strand at 342.807 kb on - strand at 342.808 kb on + strand at 342.808 kb on + strand at 342.808 kb on + strand at 342.808 kb on + strand at 342.809 kb on - strand at 342.810 kb on + strand at 342.853 kb on - strand at 342.856 kb on - strand at 342.856 kb on - strand at 342.856 kb on - strand at 342.856 kb on - strand at 342.856 kb on - strand at 342.866 kb on + strand at 342.994 kb on - strand at 343.001 kb on - strand at 343.001 kb on - strand at 343.090 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE remove 341,031 + +0.3 341,059 - -2.5 341,145 - +0.3 341,146 + -0.5 341,242 - -0.1 341,242 - +0.3 341,242 - +0.6 341,245 - +0.2 341,245 - -0.9 341,245 - +0.1 341,246 + -0.4 341,246 + -2.2 341,247 - -1.4 341,247 - -0.4 341,247 - +0.1 341,247 - +2.3 341,248 + -0.0 341,250 - +0.4 341,250 - +0.7 341,251 + +0.1 341,252 - -0.0 341,252 - +0.3 341,252 - -0.2 341,252 - -1.4 341,253 + -0.2 341,253 + +0.8 341,253 + +0.5 341,253 + -0.2 341,264 - +1.9 341,265 + -0.6 341,265 + +0.0 341,265 + -0.0 341,275 + -0.7 341,275 + +0.2 341,276 - +2.1 341,276 - +0.2 341,277 + +0.4 341,296 + +1.4 341,308 + -1.1 341,322 - +0.7 341,348 - MMJJ_RS01760 0.13 -2.3 341,349 + MMJJ_RS01760 0.13 +0.3 341,349 + MMJJ_RS01760 0.13 -0.9 341,349 + MMJJ_RS01760 0.13 -0.4 341,349 + MMJJ_RS01760 0.13 +2.0 341,375 - MMJJ_RS01760 0.17 -1.0 341,428 + MMJJ_RS01760 0.26 +2.0 341,461 + MMJJ_RS01760 0.32 -0.3 341,463 - MMJJ_RS01760 0.32 -1.5 341,464 + MMJJ_RS01760 0.32 -0.1 341,467 - MMJJ_RS01760 0.33 -0.9 341,483 - MMJJ_RS01760 0.35 +0.7 341,484 + MMJJ_RS01760 0.35 -0.1 341,484 + MMJJ_RS01760 0.35 -0.7 341,529 - MMJJ_RS01760 0.43 +0.5 341,530 + MMJJ_RS01760 0.43 +1.5 341,544 - MMJJ_RS01760 0.45 -0.6 341,610 + MMJJ_RS01760 0.56 +0.2 341,610 + MMJJ_RS01760 0.56 +1.7 341,647 - MMJJ_RS01760 0.62 +2.3 341,647 - MMJJ_RS01760 0.62 +1.1 341,648 + MMJJ_RS01760 0.62 +0.1 341,648 + MMJJ_RS01760 0.62 -0.4 341,667 - MMJJ_RS01760 0.65 +1.1 341,760 - MMJJ_RS01760 0.81 +0.1 341,777 + MMJJ_RS01760 0.83 -1.0 341,777 + MMJJ_RS01760 0.83 -0.2 341,781 + MMJJ_RS01760 0.84 +0.3 341,795 - MMJJ_RS01760 0.86 +1.5 341,796 + MMJJ_RS01760 0.87 +1.7 341,796 + MMJJ_RS01760 0.87 +1.0 341,800 + MMJJ_RS01760 0.87 +0.0 341,840 + +0.7 341,841 - -0.3 341,857 + -0.8 341,871 + -0.1 341,871 + +0.4 341,876 + +0.0 341,884 - +1.7 341,927 - -0.5 341,931 - +1.5 341,931 - +0.0 341,962 + +0.9 341,996 - -2.6 341,997 + -3.2 342,079 + MMJJ_RS01765 0.25 -1.3 342,079 + MMJJ_RS01765 0.25 -3.1 342,080 - MMJJ_RS01765 0.25 -2.6 342,081 + MMJJ_RS01765 0.26 -3.4 342,100 + MMJJ_RS01765 0.30 -2.0 342,103 + MMJJ_RS01765 0.31 -2.5 342,103 + MMJJ_RS01765 0.31 -0.8 342,181 - MMJJ_RS01765 0.51 -3.5 342,182 + MMJJ_RS01765 0.51 -1.7 342,193 - MMJJ_RS01765 0.54 -2.2 342,193 - MMJJ_RS01765 0.54 -2.0 342,194 + MMJJ_RS01765 0.54 -2.3 342,194 + MMJJ_RS01765 0.54 -3.5 342,205 + MMJJ_RS01765 0.57 -4.1 342,219 + MMJJ_RS01765 0.60 -2.8 342,219 + MMJJ_RS01765 0.60 -3.2 342,231 - MMJJ_RS01765 0.63 -1.4 342,232 + MMJJ_RS01765 0.63 -2.0 342,232 + MMJJ_RS01765 0.63 -2.0 342,288 - MMJJ_RS01765 0.77 -2.8 342,288 - MMJJ_RS01765 0.77 -2.3 342,289 + MMJJ_RS01765 0.78 -4.6 342,318 - MMJJ_RS01765 0.85 -3.9 342,318 - MMJJ_RS01765 0.85 -2.3 342,330 - MMJJ_RS01765 0.88 -1.4 342,330 - MMJJ_RS01765 0.88 -3.5 342,330 - MMJJ_RS01765 0.88 -1.5 342,331 + MMJJ_RS01765 0.88 -3.3 342,331 + MMJJ_RS01765 0.88 -2.9 342,371 - +1.3 342,371 - -4.6 342,372 + -3.9 342,377 - -2.4 342,378 + -5.4 342,406 - -3.6 342,407 + -3.1 342,430 + -2.4 342,430 + -1.2 342,433 - -3.4 342,433 - -1.0 342,434 + -3.4 342,434 + -1.6 342,435 - -4.5 342,436 + -0.9 342,436 + -2.4 342,437 - -2.4 342,438 + -5.7 342,438 + -2.4 342,438 + -2.5 342,438 + -3.8 342,439 - -3.6 342,440 + -1.8 342,443 - -2.3 342,443 - -0.6 342,459 + -1.4 342,460 - -1.0 342,463 - -2.0 342,464 + -4.0 342,464 + -3.3 342,476 + -4.1 342,477 - -1.9 342,477 - +0.3 342,477 - -1.8 342,478 + -1.6 342,478 + -2.0 342,478 + -2.9 342,478 + -2.5 342,478 + -1.6 342,479 - -2.1 342,480 + -1.0 342,480 + -2.8 342,499 - -1.1 342,587 - MMJJ_RS01770 0.20 +0.3 342,634 - MMJJ_RS01770 0.43 +1.6 342,638 - MMJJ_RS01770 0.45 +0.4 342,653 + MMJJ_RS01770 0.52 -0.8 342,689 - MMJJ_RS01770 0.70 -0.2 342,690 + MMJJ_RS01770 0.70 -0.0 342,753 - +0.1 342,753 - -0.8 342,753 - +0.2 342,754 + +0.6 342,754 + +1.3 342,754 + +0.5 342,754 + -0.2 342,754 + -0.8 342,754 + +1.0 342,754 + +0.8 342,776 - -0.5 342,780 - +0.9 342,780 - -1.3 342,785 - -0.5 342,785 - -1.3 342,788 + -0.5 342,800 + +0.6 342,807 - -0.0 342,807 - +0.4 342,807 - +0.0 342,808 + +0.3 342,808 + +0.0 342,808 + +1.2 342,808 + +0.3 342,809 - -1.4 342,810 + -0.1 342,853 - +0.1 342,856 - +1.6 342,856 - +0.6 342,856 - -0.8 342,856 - -0.2 342,856 - -0.1 342,866 + +0.3 342,994 - -0.9 343,001 - +0.0 343,001 - -0.2 343,090 - +0.1
Or see this region's nucleotide sequence