Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMc00074 and SMc00075 overlap by 1 nucleotides SMc00075 and SMc00451 are separated by 271 nucleotides SMc00451 and SMc00077 are separated by 18 nucleotides
SMc00074: SMc00074 - transmembrane signal peptide protein, at 1,003,924 to 1,006,836
SMc00074
SMc00075: SMc00075 - ribosomal-protein-alanine acetyltransferase, at 1,006,836 to 1,007,447
SMc00075
SMc00451: SMc00451 - processing protease, at 1,007,719 to 1,009,017
SMc00451
SMc00077: SMc00077 - threonine synthase, at 1,009,036 to 1,010,436
SMc00077
Position (kb)
1007
1008
1009
1010 Strain fitness (log2 ratio)
-2
-1
0
1 at 1006.925 kb on - strand, within SMc00075 at 1006.925 kb on - strand, within SMc00075 at 1006.937 kb on - strand, within SMc00075 at 1007.042 kb on - strand, within SMc00075 at 1007.069 kb on - strand, within SMc00075 at 1007.069 kb on - strand, within SMc00075 at 1007.073 kb on - strand, within SMc00075 at 1007.381 kb on - strand, within SMc00075 at 1007.450 kb on - strand at 1007.450 kb on - strand at 1007.450 kb on - strand at 1007.669 kb on - strand at 1007.737 kb on + strand at 1007.770 kb on + strand at 1007.771 kb on - strand at 1008.254 kb on + strand, within SMc00451 at 1008.254 kb on + strand, within SMc00451 at 1008.255 kb on - strand, within SMc00451 at 1008.477 kb on + strand, within SMc00451 at 1008.478 kb on - strand, within SMc00451 at 1008.478 kb on - strand, within SMc00451 at 1008.480 kb on - strand, within SMc00451 at 1008.480 kb on - strand, within SMc00451 at 1008.576 kb on - strand, within SMc00451 at 1008.755 kb on + strand, within SMc00451 at 1008.758 kb on + strand, within SMc00451 at 1008.758 kb on + strand, within SMc00451 at 1008.758 kb on + strand, within SMc00451 at 1008.759 kb on - strand, within SMc00451 at 1009.054 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,006,925 - SMc00075 0.15 +1.6 1,006,925 - SMc00075 0.15 -0.0 1,006,937 - SMc00075 0.17 +0.8 1,007,042 - SMc00075 0.34 +1.1 1,007,069 - SMc00075 0.38 +1.0 1,007,069 - SMc00075 0.38 +1.6 1,007,073 - SMc00075 0.39 +1.3 1,007,381 - SMc00075 0.89 -0.2 1,007,450 - -2.0 1,007,450 - -2.6 1,007,450 - -1.4 1,007,669 - +0.9 1,007,737 + -1.5 1,007,770 + -0.0 1,007,771 - +1.6 1,008,254 + SMc00451 0.41 +0.1 1,008,254 + SMc00451 0.41 -1.4 1,008,255 - SMc00451 0.41 -0.3 1,008,477 + SMc00451 0.58 -2.3 1,008,478 - SMc00451 0.58 -1.1 1,008,478 - SMc00451 0.58 -2.2 1,008,480 - SMc00451 0.59 -0.5 1,008,480 - SMc00451 0.59 -0.6 1,008,576 - SMc00451 0.66 -0.5 1,008,755 + SMc00451 0.80 -2.0 1,008,758 + SMc00451 0.80 -0.6 1,008,758 + SMc00451 0.80 -2.3 1,008,758 + SMc00451 0.80 -0.2 1,008,759 - SMc00451 0.80 -1.1 1,009,054 - -0.0
Or see this region's nucleotide sequence