Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMc00016 and SMc00017 are separated by 154 nucleotides SMc00017 and SMc00018 overlap by 4 nucleotides SMc00018 and SMc00072 are separated by 210 nucleotides
SMc00016: SMc00016 - 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, at 998,733 to 999,785
SMc00016
SMc00017: SMc00017 - homoserine kinase, at 999,940 to 1,000,920
SMc00017
SMc00018: SMc00018 - ribonuclease H, at 1,000,917 to 1,001,378
SMc00018
SMc00072: SMc00072 - peroxiredoxin protein, at 1,001,589 to 1,002,074
SMc00072
Position (kb) 999
1000
1001 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 999.792 kb on + strand at 999.792 kb on + strand at 999.869 kb on + strand at 999.869 kb on + strand at 999.894 kb on - strand at 999.915 kb on + strand at 999.915 kb on + strand at 999.916 kb on - strand at 999.916 kb on - strand at 1000.018 kb on + strand at 1000.081 kb on + strand, within SMc00017 at 1000.083 kb on + strand, within SMc00017 at 1000.888 kb on + strand at 1001.376 kb on + strand at 1001.377 kb on - strand at 1001.756 kb on + strand, within SMc00072
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 999,792 + -1.0 999,792 + -0.0 999,869 + -0.0 999,869 + +0.1 999,894 - -1.1 999,915 + -0.1 999,915 + -0.2 999,916 - -2.5 999,916 - +0.1 1,000,018 + +2.1 1,000,081 + SMc00017 0.14 +2.0 1,000,083 + SMc00017 0.15 +2.9 1,000,888 + -0.5 1,001,376 + -0.1 1,001,377 - +1.4 1,001,756 + SMc00072 0.34 -0.5
Or see this region's nucleotide sequence