Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b20004 and SM_b20005 are separated by 115 nucleotides SM_b20005 and SM_b20006 are separated by 185 nucleotides
SM_b20004: SM_b20004 - gstR-like transcriptional regulator protein, at 7,268 to 8,173
_b20004
SM_b20005: SM_b20005 - glutathione S-transferase, at 8,289 to 8,900
_b20005
SM_b20006: SM_b20006 - hypothetical protein, at 9,086 to 10,039
_b20006
Position (kb)
8
9 Strain fitness (log2 ratio)
-2
-1
0
1 at 7.549 kb on + strand, within SM_b20004 at 7.806 kb on - strand, within SM_b20004 at 7.813 kb on + strand, within SM_b20004 at 7.980 kb on + strand, within SM_b20004 at 7.981 kb on - strand, within SM_b20004 at 7.981 kb on - strand, within SM_b20004 at 7.981 kb on - strand, within SM_b20004 at 7.981 kb on - strand, within SM_b20004 at 8.092 kb on + strand at 8.093 kb on - strand at 8.093 kb on - strand at 8.298 kb on + strand at 8.298 kb on + strand at 8.298 kb on + strand at 8.299 kb on - strand at 8.499 kb on + strand, within SM_b20005 at 8.764 kb on - strand, within SM_b20005 at 8.770 kb on - strand, within SM_b20005 at 8.774 kb on - strand, within SM_b20005 at 8.812 kb on - strand, within SM_b20005 at 9.500 kb on - strand, within SM_b20006 at 9.561 kb on - strand, within SM_b20006 at 9.695 kb on + strand, within SM_b20006
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 7,549 + SM_b20004 0.31 -0.7 7,806 - SM_b20004 0.59 -0.2 7,813 + SM_b20004 0.60 -0.3 7,980 + SM_b20004 0.79 +0.3 7,981 - SM_b20004 0.79 -0.6 7,981 - SM_b20004 0.79 -1.0 7,981 - SM_b20004 0.79 +0.0 7,981 - SM_b20004 0.79 -0.2 8,092 + -0.7 8,093 - -0.5 8,093 - -0.8 8,298 + +0.1 8,298 + -0.4 8,298 + -1.0 8,299 - +0.1 8,499 + SM_b20005 0.34 -0.4 8,764 - SM_b20005 0.78 -2.5 8,770 - SM_b20005 0.79 -1.9 8,774 - SM_b20005 0.79 -0.2 8,812 - SM_b20005 0.85 -0.3 9,500 - SM_b20006 0.43 -0.3 9,561 - SM_b20006 0.50 -2.0 9,695 + SM_b20006 0.64 -0.7
Or see this region's nucleotide sequence