Experiment: a-Ketoglutaric (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS25640 and RR42_RS25645 are separated by 115 nucleotides RR42_RS25645 and RR42_RS25650 are separated by 316 nucleotides RR42_RS25650 and RR42_RS25655 are separated by 95 nucleotides RR42_RS25655 and RR42_RS25660 are separated by 8 nucleotides
RR42_RS25640: RR42_RS25640 - 3-hydroxyisobutyrate dehydrogenase, at 1,096,083 to 1,096,979
_RS25640
RR42_RS25645: RR42_RS25645 - hypothetical protein, at 1,097,095 to 1,097,289
_RS25645
RR42_RS25650: RR42_RS25650 - hypothetical protein, at 1,097,606 to 1,097,806
_RS25650
RR42_RS25655: RR42_RS25655 - pyruvate carboxylase, at 1,097,902 to 1,101,411
_RS25655
RR42_RS25660: RR42_RS25660 - cytochrome C biogenesis protein, at 1,101,420 to 1,102,091
_RS25660
Position (kb)
1097
1098
1099
1100
1101
1102 Strain fitness (log2 ratio)
-2
-1
0
1 at 1097.089 kb on + strand at 1097.089 kb on + strand at 1097.089 kb on + strand at 1097.097 kb on - strand at 1097.250 kb on + strand, within RR42_RS25645 at 1097.250 kb on + strand, within RR42_RS25645 at 1097.251 kb on - strand, within RR42_RS25645 at 1097.251 kb on - strand, within RR42_RS25645 at 1097.294 kb on + strand at 1097.295 kb on - strand at 1097.295 kb on - strand at 1097.295 kb on - strand at 1097.338 kb on + strand at 1097.338 kb on + strand at 1097.338 kb on + strand at 1097.339 kb on - strand at 1097.339 kb on - strand at 1097.460 kb on - strand at 1097.610 kb on - strand at 1097.610 kb on - strand at 1097.610 kb on - strand at 1097.613 kb on - strand at 1097.613 kb on - strand at 1097.704 kb on + strand, within RR42_RS25650 at 1097.704 kb on + strand, within RR42_RS25650 at 1097.798 kb on + strand at 1097.799 kb on - strand at 1097.799 kb on - strand at 1097.901 kb on + strand at 1097.908 kb on + strand at 1097.908 kb on + strand at 1097.989 kb on - strand at 1098.000 kb on - strand at 1098.007 kb on + strand at 1098.059 kb on - strand at 1098.095 kb on - strand at 1098.508 kb on + strand, within RR42_RS25655 at 1098.508 kb on + strand, within RR42_RS25655 at 1098.508 kb on + strand, within RR42_RS25655 at 1098.509 kb on - strand, within RR42_RS25655 at 1098.509 kb on - strand, within RR42_RS25655 at 1098.589 kb on + strand, within RR42_RS25655 at 1098.589 kb on + strand, within RR42_RS25655 at 1098.590 kb on - strand, within RR42_RS25655 at 1098.590 kb on - strand, within RR42_RS25655 at 1098.603 kb on + strand, within RR42_RS25655 at 1098.604 kb on - strand, within RR42_RS25655 at 1098.604 kb on - strand, within RR42_RS25655 at 1098.772 kb on + strand, within RR42_RS25655 at 1098.772 kb on + strand, within RR42_RS25655 at 1098.773 kb on - strand, within RR42_RS25655 at 1098.810 kb on + strand, within RR42_RS25655 at 1098.811 kb on - strand, within RR42_RS25655 at 1098.811 kb on - strand, within RR42_RS25655 at 1099.030 kb on + strand, within RR42_RS25655 at 1099.123 kb on + strand, within RR42_RS25655 at 1099.366 kb on + strand, within RR42_RS25655 at 1099.624 kb on - strand, within RR42_RS25655 at 1099.825 kb on + strand, within RR42_RS25655 at 1099.825 kb on + strand, within RR42_RS25655 at 1099.826 kb on - strand, within RR42_RS25655 at 1100.018 kb on - strand, within RR42_RS25655 at 1100.018 kb on - strand, within RR42_RS25655 at 1100.029 kb on + strand, within RR42_RS25655 at 1100.029 kb on + strand, within RR42_RS25655 at 1100.030 kb on - strand, within RR42_RS25655 at 1100.030 kb on - strand, within RR42_RS25655 at 1100.032 kb on + strand, within RR42_RS25655 at 1100.033 kb on - strand, within RR42_RS25655 at 1100.287 kb on - strand, within RR42_RS25655 at 1100.372 kb on - strand, within RR42_RS25655 at 1100.372 kb on - strand, within RR42_RS25655 at 1100.372 kb on - strand, within RR42_RS25655 at 1100.372 kb on - strand, within RR42_RS25655 at 1100.384 kb on + strand, within RR42_RS25655 at 1100.393 kb on - strand, within RR42_RS25655 at 1100.395 kb on + strand, within RR42_RS25655 at 1100.395 kb on + strand, within RR42_RS25655 at 1100.536 kb on + strand, within RR42_RS25655 at 1100.537 kb on - strand, within RR42_RS25655 at 1100.894 kb on - strand, within RR42_RS25655 at 1100.936 kb on - strand, within RR42_RS25655 at 1100.938 kb on - strand, within RR42_RS25655 at 1100.938 kb on - strand, within RR42_RS25655 at 1100.943 kb on + strand, within RR42_RS25655 at 1101.352 kb on - strand at 1101.456 kb on + strand at 1101.457 kb on - strand at 1101.642 kb on - strand, within RR42_RS25660 at 1101.663 kb on + strand, within RR42_RS25660 at 1101.664 kb on - strand, within RR42_RS25660 at 1101.673 kb on - strand, within RR42_RS25660 at 1101.732 kb on + strand, within RR42_RS25660 at 1101.994 kb on - strand, within RR42_RS25660 at 1102.072 kb on - strand at 1102.143 kb on + strand at 1102.144 kb on - strand at 1102.388 kb on + strand at 1102.389 kb on - strand at 1102.389 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction a-Ketoglutaric (C) remove 1,097,089 + +1.4 1,097,089 + -0.1 1,097,089 + +0.5 1,097,097 - +0.3 1,097,250 + RR42_RS25645 0.79 -0.7 1,097,250 + RR42_RS25645 0.79 -0.3 1,097,251 - RR42_RS25645 0.80 -0.1 1,097,251 - RR42_RS25645 0.80 -1.1 1,097,294 + -0.8 1,097,295 - +0.3 1,097,295 - +1.0 1,097,295 - -1.3 1,097,338 + -0.2 1,097,338 + +0.2 1,097,338 + -1.3 1,097,339 - +0.5 1,097,339 - -0.6 1,097,460 - -0.5 1,097,610 - -0.4 1,097,610 - +1.2 1,097,610 - +0.7 1,097,613 - -0.0 1,097,613 - -1.2 1,097,704 + RR42_RS25650 0.49 +0.1 1,097,704 + RR42_RS25650 0.49 +1.2 1,097,798 + +0.1 1,097,799 - -0.1 1,097,799 - -0.3 1,097,901 + +0.1 1,097,908 + -0.0 1,097,908 + +0.0 1,097,989 - +0.4 1,098,000 - -0.3 1,098,007 + +0.8 1,098,059 - -0.4 1,098,095 - +0.4 1,098,508 + RR42_RS25655 0.17 +0.8 1,098,508 + RR42_RS25655 0.17 +0.7 1,098,508 + RR42_RS25655 0.17 -0.6 1,098,509 - RR42_RS25655 0.17 -0.4 1,098,509 - RR42_RS25655 0.17 -0.1 1,098,589 + RR42_RS25655 0.20 +0.7 1,098,589 + RR42_RS25655 0.20 -0.6 1,098,590 - RR42_RS25655 0.20 +0.6 1,098,590 - RR42_RS25655 0.20 +0.5 1,098,603 + RR42_RS25655 0.20 -0.2 1,098,604 - RR42_RS25655 0.20 -1.3 1,098,604 - RR42_RS25655 0.20 -1.4 1,098,772 + RR42_RS25655 0.25 +0.6 1,098,772 + RR42_RS25655 0.25 -0.9 1,098,773 - RR42_RS25655 0.25 +0.9 1,098,810 + RR42_RS25655 0.26 -0.1 1,098,811 - RR42_RS25655 0.26 -0.6 1,098,811 - RR42_RS25655 0.26 -0.3 1,099,030 + RR42_RS25655 0.32 -0.5 1,099,123 + RR42_RS25655 0.35 -0.4 1,099,366 + RR42_RS25655 0.42 +0.7 1,099,624 - RR42_RS25655 0.49 -0.3 1,099,825 + RR42_RS25655 0.55 -0.2 1,099,825 + RR42_RS25655 0.55 -0.7 1,099,826 - RR42_RS25655 0.55 -0.2 1,100,018 - RR42_RS25655 0.60 -0.0 1,100,018 - RR42_RS25655 0.60 -0.5 1,100,029 + RR42_RS25655 0.61 +0.3 1,100,029 + RR42_RS25655 0.61 +0.7 1,100,030 - RR42_RS25655 0.61 -0.7 1,100,030 - RR42_RS25655 0.61 +1.3 1,100,032 + RR42_RS25655 0.61 +0.4 1,100,033 - RR42_RS25655 0.61 -0.3 1,100,287 - RR42_RS25655 0.68 -1.1 1,100,372 - RR42_RS25655 0.70 +0.8 1,100,372 - RR42_RS25655 0.70 -0.4 1,100,372 - RR42_RS25655 0.70 -1.0 1,100,372 - RR42_RS25655 0.70 -0.3 1,100,384 + RR42_RS25655 0.71 +0.0 1,100,393 - RR42_RS25655 0.71 +1.3 1,100,395 + RR42_RS25655 0.71 +0.4 1,100,395 + RR42_RS25655 0.71 -0.6 1,100,536 + RR42_RS25655 0.75 -0.7 1,100,537 - RR42_RS25655 0.75 -0.2 1,100,894 - RR42_RS25655 0.85 -0.2 1,100,936 - RR42_RS25655 0.86 -0.1 1,100,938 - RR42_RS25655 0.86 -1.6 1,100,938 - RR42_RS25655 0.86 -0.3 1,100,943 + RR42_RS25655 0.87 +0.9 1,101,352 - -0.3 1,101,456 + -0.8 1,101,457 - +0.1 1,101,642 - RR42_RS25660 0.33 -0.6 1,101,663 + RR42_RS25660 0.36 +0.1 1,101,664 - RR42_RS25660 0.36 -1.8 1,101,673 - RR42_RS25660 0.38 +0.1 1,101,732 + RR42_RS25660 0.46 +0.7 1,101,994 - RR42_RS25660 0.85 +1.1 1,102,072 - -0.1 1,102,143 + +0.1 1,102,144 - +0.7 1,102,388 + +0.0 1,102,389 - -2.6 1,102,389 - +0.5
Or see this region's nucleotide sequence