Experiment: a-Ketoglutaric (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00875 and RR42_RS00880 overlap by 4 nucleotides RR42_RS00880 and RR42_RS00885 are separated by 93 nucleotides
RR42_RS00875: RR42_RS00875 - isocitrate dehydrogenase, at 211,201 to 213,045
_RS00875
RR42_RS00880: RR42_RS00880 - carbonate dehydratase, at 213,042 to 213,713
_RS00880
RR42_RS00885: RR42_RS00885 - acetyl-CoA acetyltransferase, at 213,807 to 215,003
_RS00885
Position (kb) 213
214 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 212.090 kb on - strand, within RR42_RS00875 at 212.090 kb on - strand, within RR42_RS00875 at 212.236 kb on + strand, within RR42_RS00875 at 212.236 kb on + strand, within RR42_RS00875 at 212.236 kb on + strand, within RR42_RS00875 at 212.237 kb on - strand, within RR42_RS00875 at 212.261 kb on - strand, within RR42_RS00875 at 212.261 kb on - strand, within RR42_RS00875 at 212.309 kb on - strand, within RR42_RS00875 at 212.447 kb on - strand, within RR42_RS00875 at 212.494 kb on + strand, within RR42_RS00875 at 212.494 kb on + strand, within RR42_RS00875 at 212.495 kb on - strand, within RR42_RS00875 at 212.495 kb on - strand, within RR42_RS00875 at 212.496 kb on + strand, within RR42_RS00875 at 212.496 kb on + strand, within RR42_RS00875 at 212.610 kb on + strand, within RR42_RS00875 at 212.632 kb on + strand, within RR42_RS00875 at 212.632 kb on + strand, within RR42_RS00875 at 212.632 kb on + strand, within RR42_RS00875 at 212.632 kb on + strand, within RR42_RS00875 at 212.632 kb on + strand, within RR42_RS00875 at 212.941 kb on + strand at 212.941 kb on + strand at 212.941 kb on + strand at 212.941 kb on + strand at 213.739 kb on + strand at 213.739 kb on + strand at 213.743 kb on + strand at 213.743 kb on + strand at 213.744 kb on - strand at 213.820 kb on + strand at 213.820 kb on + strand at 214.263 kb on + strand, within RR42_RS00885 at 214.264 kb on - strand, within RR42_RS00885 at 214.264 kb on - strand, within RR42_RS00885 at 214.329 kb on + strand, within RR42_RS00885 at 214.329 kb on + strand, within RR42_RS00885 at 214.329 kb on + strand, within RR42_RS00885 at 214.329 kb on + strand, within RR42_RS00885 at 214.329 kb on + strand, within RR42_RS00885 at 214.329 kb on + strand, within RR42_RS00885 at 214.338 kb on - strand, within RR42_RS00885
Per-strain Table
Position Strand Gene LocusTag Fraction a-Ketoglutaric (C) remove 212,090 - RR42_RS00875 0.48 -0.2 212,090 - RR42_RS00875 0.48 -2.0 212,236 + RR42_RS00875 0.56 -2.1 212,236 + RR42_RS00875 0.56 -0.4 212,236 + RR42_RS00875 0.56 -1.8 212,237 - RR42_RS00875 0.56 -3.0 212,261 - RR42_RS00875 0.57 -2.2 212,261 - RR42_RS00875 0.57 -1.0 212,309 - RR42_RS00875 0.60 -1.8 212,447 - RR42_RS00875 0.68 -0.8 212,494 + RR42_RS00875 0.70 -0.8 212,494 + RR42_RS00875 0.70 -0.3 212,495 - RR42_RS00875 0.70 -2.6 212,495 - RR42_RS00875 0.70 +0.1 212,496 + RR42_RS00875 0.70 -0.3 212,496 + RR42_RS00875 0.70 -1.8 212,610 + RR42_RS00875 0.76 -1.9 212,632 + RR42_RS00875 0.78 -1.4 212,632 + RR42_RS00875 0.78 -1.7 212,632 + RR42_RS00875 0.78 -2.0 212,632 + RR42_RS00875 0.78 -1.7 212,632 + RR42_RS00875 0.78 -2.2 212,941 + -1.5 212,941 + -1.4 212,941 + -1.2 212,941 + -0.9 213,739 + -0.1 213,739 + +0.1 213,743 + +0.6 213,743 + -0.3 213,744 - -0.9 213,820 + +0.9 213,820 + +1.4 214,263 + RR42_RS00885 0.38 -2.5 214,264 - RR42_RS00885 0.38 -0.1 214,264 - RR42_RS00885 0.38 -0.3 214,329 + RR42_RS00885 0.44 +0.7 214,329 + RR42_RS00885 0.44 +0.5 214,329 + RR42_RS00885 0.44 +0.8 214,329 + RR42_RS00885 0.44 -0.5 214,329 + RR42_RS00885 0.44 +0.4 214,329 + RR42_RS00885 0.44 -0.3 214,338 - RR42_RS00885 0.44 -1.7
Or see this region's nucleotide sequence