Experiment: CDM1 without Lysine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS16465 and BBR_RS16470 are separated by 43 nucleotides BBR_RS16470 and BBR_RS16475 are separated by 166 nucleotides
BBR_RS16465: BBR_RS16465 - ribokinase, at 1,457,126 to 1,458,118
_RS16465
BBR_RS16470: BBR_RS16470 - MFS transporter, at 1,458,162 to 1,459,370
_RS16470
BBR_RS16475: BBR_RS16475 - type IIA DNA topoisomerase subunit B, at 1,459,537 to 1,461,888
_RS16475
Position (kb)
1458
1459
1460 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1457.206 kb on + strand at 1457.211 kb on + strand at 1457.211 kb on + strand at 1457.228 kb on + strand, within BBR_RS16465 at 1457.234 kb on - strand, within BBR_RS16465 at 1457.245 kb on - strand, within BBR_RS16465 at 1457.307 kb on + strand, within BBR_RS16465 at 1457.307 kb on + strand, within BBR_RS16465 at 1457.307 kb on + strand, within BBR_RS16465 at 1457.307 kb on + strand, within BBR_RS16465 at 1457.315 kb on - strand, within BBR_RS16465 at 1457.315 kb on - strand, within BBR_RS16465 at 1457.315 kb on - strand, within BBR_RS16465 at 1457.317 kb on + strand, within BBR_RS16465 at 1457.356 kb on + strand, within BBR_RS16465 at 1457.364 kb on - strand, within BBR_RS16465 at 1457.425 kb on + strand, within BBR_RS16465 at 1457.433 kb on - strand, within BBR_RS16465 at 1457.489 kb on - strand, within BBR_RS16465 at 1457.514 kb on + strand, within BBR_RS16465 at 1457.514 kb on + strand, within BBR_RS16465 at 1457.556 kb on + strand, within BBR_RS16465 at 1457.563 kb on - strand, within BBR_RS16465 at 1457.722 kb on + strand, within BBR_RS16465 at 1457.831 kb on - strand, within BBR_RS16465 at 1457.862 kb on + strand, within BBR_RS16465 at 1457.916 kb on + strand, within BBR_RS16465 at 1457.954 kb on + strand, within BBR_RS16465 at 1457.955 kb on + strand, within BBR_RS16465 at 1457.957 kb on - strand, within BBR_RS16465 at 1457.957 kb on - strand, within BBR_RS16465 at 1457.962 kb on - strand, within BBR_RS16465 at 1457.968 kb on + strand, within BBR_RS16465 at 1457.973 kb on + strand, within BBR_RS16465 at 1457.995 kb on + strand, within BBR_RS16465 at 1457.998 kb on + strand, within BBR_RS16465 at 1458.000 kb on + strand, within BBR_RS16465 at 1458.008 kb on - strand, within BBR_RS16465 at 1458.059 kb on + strand at 1458.144 kb on + strand at 1458.184 kb on + strand at 1458.247 kb on + strand at 1458.247 kb on + strand at 1458.255 kb on - strand at 1458.303 kb on + strand, within BBR_RS16470 at 1458.311 kb on - strand, within BBR_RS16470 at 1458.357 kb on + strand, within BBR_RS16470 at 1458.382 kb on + strand, within BBR_RS16470 at 1458.382 kb on + strand, within BBR_RS16470 at 1458.384 kb on + strand, within BBR_RS16470 at 1458.390 kb on - strand, within BBR_RS16470 at 1458.390 kb on - strand, within BBR_RS16470 at 1458.390 kb on - strand, within BBR_RS16470 at 1458.392 kb on - strand, within BBR_RS16470 at 1458.405 kb on - strand, within BBR_RS16470 at 1458.425 kb on + strand, within BBR_RS16470 at 1458.428 kb on - strand, within BBR_RS16470 at 1458.439 kb on - strand, within BBR_RS16470 at 1458.465 kb on + strand, within BBR_RS16470 at 1458.480 kb on + strand, within BBR_RS16470 at 1458.537 kb on + strand, within BBR_RS16470 at 1458.552 kb on + strand, within BBR_RS16470 at 1458.568 kb on + strand, within BBR_RS16470 at 1458.568 kb on + strand, within BBR_RS16470 at 1458.571 kb on - strand, within BBR_RS16470 at 1458.576 kb on - strand, within BBR_RS16470 at 1458.626 kb on + strand, within BBR_RS16470 at 1458.626 kb on - strand, within BBR_RS16470 at 1458.642 kb on + strand, within BBR_RS16470 at 1458.739 kb on + strand, within BBR_RS16470 at 1458.748 kb on - strand, within BBR_RS16470 at 1458.771 kb on - strand, within BBR_RS16470 at 1458.779 kb on - strand, within BBR_RS16470 at 1458.893 kb on + strand, within BBR_RS16470 at 1458.920 kb on - strand, within BBR_RS16470 at 1458.950 kb on + strand, within BBR_RS16470 at 1459.166 kb on - strand, within BBR_RS16470 at 1459.199 kb on - strand, within BBR_RS16470 at 1459.205 kb on - strand, within BBR_RS16470 at 1459.266 kb on + strand at 1459.271 kb on + strand at 1459.300 kb on - strand at 1459.342 kb on - strand at 1459.425 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction CDM1 without Lysine remove 1,457,206 + +0.3 1,457,211 + -1.1 1,457,211 + +1.6 1,457,228 + BBR_RS16465 0.10 +0.4 1,457,234 - BBR_RS16465 0.11 -1.2 1,457,245 - BBR_RS16465 0.12 +0.9 1,457,307 + BBR_RS16465 0.18 -0.5 1,457,307 + BBR_RS16465 0.18 +0.5 1,457,307 + BBR_RS16465 0.18 +0.9 1,457,307 + BBR_RS16465 0.18 +0.2 1,457,315 - BBR_RS16465 0.19 +1.7 1,457,315 - BBR_RS16465 0.19 -0.0 1,457,315 - BBR_RS16465 0.19 -1.0 1,457,317 + BBR_RS16465 0.19 +1.9 1,457,356 + BBR_RS16465 0.23 +0.8 1,457,364 - BBR_RS16465 0.24 -1.3 1,457,425 + BBR_RS16465 0.30 +0.5 1,457,433 - BBR_RS16465 0.31 -1.0 1,457,489 - BBR_RS16465 0.37 +0.9 1,457,514 + BBR_RS16465 0.39 +1.6 1,457,514 + BBR_RS16465 0.39 -0.8 1,457,556 + BBR_RS16465 0.43 +0.3 1,457,563 - BBR_RS16465 0.44 -0.5 1,457,722 + BBR_RS16465 0.60 +0.0 1,457,831 - BBR_RS16465 0.71 +1.4 1,457,862 + BBR_RS16465 0.74 -0.2 1,457,916 + BBR_RS16465 0.80 +0.7 1,457,954 + BBR_RS16465 0.83 -2.9 1,457,955 + BBR_RS16465 0.83 -0.7 1,457,957 - BBR_RS16465 0.84 +1.0 1,457,957 - BBR_RS16465 0.84 -2.0 1,457,962 - BBR_RS16465 0.84 +0.0 1,457,968 + BBR_RS16465 0.85 -0.2 1,457,973 + BBR_RS16465 0.85 -0.9 1,457,995 + BBR_RS16465 0.88 +0.2 1,457,998 + BBR_RS16465 0.88 -1.8 1,458,000 + BBR_RS16465 0.88 -2.9 1,458,008 - BBR_RS16465 0.89 -0.4 1,458,059 + -1.9 1,458,144 + +1.0 1,458,184 + -3.9 1,458,247 + -1.4 1,458,247 + -0.4 1,458,255 - +0.3 1,458,303 + BBR_RS16470 0.12 -2.8 1,458,311 - BBR_RS16470 0.12 -0.5 1,458,357 + BBR_RS16470 0.16 -0.6 1,458,382 + BBR_RS16470 0.18 -0.6 1,458,382 + BBR_RS16470 0.18 -1.5 1,458,384 + BBR_RS16470 0.18 -1.5 1,458,390 - BBR_RS16470 0.19 +0.2 1,458,390 - BBR_RS16470 0.19 -0.7 1,458,390 - BBR_RS16470 0.19 -3.6 1,458,392 - BBR_RS16470 0.19 +0.2 1,458,405 - BBR_RS16470 0.20 +0.0 1,458,425 + BBR_RS16470 0.22 +0.4 1,458,428 - BBR_RS16470 0.22 -0.6 1,458,439 - BBR_RS16470 0.23 +2.2 1,458,465 + BBR_RS16470 0.25 -2.9 1,458,480 + BBR_RS16470 0.26 +0.9 1,458,537 + BBR_RS16470 0.31 -1.0 1,458,552 + BBR_RS16470 0.32 -0.2 1,458,568 + BBR_RS16470 0.34 -0.8 1,458,568 + BBR_RS16470 0.34 -1.4 1,458,571 - BBR_RS16470 0.34 -1.0 1,458,576 - BBR_RS16470 0.34 +0.2 1,458,626 + BBR_RS16470 0.38 -1.2 1,458,626 - BBR_RS16470 0.38 -1.8 1,458,642 + BBR_RS16470 0.40 -0.5 1,458,739 + BBR_RS16470 0.48 -2.0 1,458,748 - BBR_RS16470 0.48 -2.3 1,458,771 - BBR_RS16470 0.50 -1.5 1,458,779 - BBR_RS16470 0.51 -1.1 1,458,893 + BBR_RS16470 0.60 -1.1 1,458,920 - BBR_RS16470 0.63 -1.2 1,458,950 + BBR_RS16470 0.65 -1.0 1,459,166 - BBR_RS16470 0.83 -2.6 1,459,199 - BBR_RS16470 0.86 +0.7 1,459,205 - BBR_RS16470 0.86 -0.1 1,459,266 + -2.4 1,459,271 + -2.7 1,459,300 - +0.5 1,459,342 - +1.0 1,459,425 - +0.8
Or see this region's nucleotide sequence