Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS04655 and MMJJ_RS04660 are separated by 31 nucleotides MMJJ_RS04660 and MMJJ_RS04665 are separated by 15 nucleotides MMJJ_RS04665 and MMJJ_RS04670 are separated by 27 nucleotides
MMJJ_RS04655: MMJJ_RS04655 - FAD-dependent oxidoreductase, at 868,793 to 870,199
_RS04655
MMJJ_RS04660: MMJJ_RS04660 - hypoxanthine/guanine phosphoribosyltransferase, at 870,231 to 870,788
_RS04660
MMJJ_RS04665: MMJJ_RS04665 - fumarylacetoacetate hydrolase family protein, at 870,804 to 871,412
_RS04665
MMJJ_RS04670: MMJJ_RS04670 - DUF2117 domain-containing protein, at 871,440 to 872,555
_RS04670
Position (kb)
870
871 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 869.347 kb on - strand, within MMJJ_RS04655 at 869.348 kb on + strand, within MMJJ_RS04655 at 869.349 kb on - strand at 869.349 kb on - strand, within MMJJ_RS04655 at 869.351 kb on - strand, within MMJJ_RS04655 at 869.451 kb on - strand at 869.480 kb on + strand, within MMJJ_RS04655 at 869.545 kb on - strand at 869.551 kb on - strand, within MMJJ_RS04655 at 869.618 kb on + strand, within MMJJ_RS04655 at 869.653 kb on - strand, within MMJJ_RS04655 at 869.653 kb on - strand, within MMJJ_RS04655 at 869.690 kb on + strand, within MMJJ_RS04655 at 869.994 kb on + strand, within MMJJ_RS04655 at 869.994 kb on + strand, within MMJJ_RS04655 at 870.167 kb on - strand at 870.215 kb on - strand at 870.228 kb on - strand at 870.228 kb on - strand at 870.229 kb on + strand at 870.299 kb on - strand, within MMJJ_RS04660 at 870.300 kb on + strand, within MMJJ_RS04660 at 870.300 kb on + strand, within MMJJ_RS04660 at 870.310 kb on - strand, within MMJJ_RS04660 at 870.371 kb on - strand, within MMJJ_RS04660 at 870.371 kb on - strand, within MMJJ_RS04660 at 870.380 kb on + strand, within MMJJ_RS04660 at 870.380 kb on + strand, within MMJJ_RS04660 at 870.383 kb on - strand, within MMJJ_RS04660 at 870.384 kb on + strand, within MMJJ_RS04660 at 870.384 kb on + strand, within MMJJ_RS04660 at 870.400 kb on - strand, within MMJJ_RS04660 at 870.403 kb on - strand, within MMJJ_RS04660 at 870.433 kb on - strand, within MMJJ_RS04660 at 870.465 kb on - strand, within MMJJ_RS04660 at 870.466 kb on + strand, within MMJJ_RS04660 at 870.470 kb on - strand, within MMJJ_RS04660 at 870.470 kb on - strand, within MMJJ_RS04660 at 870.470 kb on - strand, within MMJJ_RS04660 at 870.471 kb on + strand, within MMJJ_RS04660 at 870.471 kb on + strand, within MMJJ_RS04660 at 870.471 kb on + strand, within MMJJ_RS04660 at 870.471 kb on + strand, within MMJJ_RS04660 at 870.489 kb on - strand, within MMJJ_RS04660 at 870.490 kb on + strand, within MMJJ_RS04660 at 870.490 kb on + strand, within MMJJ_RS04660 at 870.549 kb on - strand, within MMJJ_RS04660 at 870.549 kb on - strand, within MMJJ_RS04660 at 870.550 kb on + strand, within MMJJ_RS04660 at 870.550 kb on + strand, within MMJJ_RS04660 at 870.553 kb on - strand, within MMJJ_RS04660 at 870.566 kb on - strand, within MMJJ_RS04660 at 870.567 kb on + strand, within MMJJ_RS04660 at 870.599 kb on - strand, within MMJJ_RS04660 at 870.602 kb on + strand, within MMJJ_RS04660 at 870.631 kb on - strand, within MMJJ_RS04660 at 870.660 kb on + strand, within MMJJ_RS04660 at 870.678 kb on + strand, within MMJJ_RS04660 at 870.678 kb on + strand, within MMJJ_RS04660 at 870.733 kb on - strand at 870.741 kb on + strand at 870.741 kb on + strand at 870.746 kb on + strand at 870.746 kb on + strand at 870.746 kb on + strand at 870.781 kb on - strand at 870.781 kb on - strand at 870.782 kb on + strand at 870.830 kb on + strand at 870.847 kb on + strand at 870.911 kb on - strand, within MMJJ_RS04665 at 870.971 kb on + strand, within MMJJ_RS04665 at 871.048 kb on - strand, within MMJJ_RS04665 at 871.048 kb on - strand, within MMJJ_RS04665 at 871.049 kb on + strand, within MMJJ_RS04665 at 871.049 kb on + strand, within MMJJ_RS04665 at 871.058 kb on - strand, within MMJJ_RS04665 at 871.066 kb on - strand, within MMJJ_RS04665 at 871.134 kb on + strand, within MMJJ_RS04665 at 871.159 kb on - strand, within MMJJ_RS04665 at 871.182 kb on + strand, within MMJJ_RS04665 at 871.246 kb on - strand, within MMJJ_RS04665 at 871.247 kb on + strand, within MMJJ_RS04665 at 871.247 kb on + strand, within MMJJ_RS04665 at 871.247 kb on + strand, within MMJJ_RS04665 at 871.310 kb on - strand, within MMJJ_RS04665 at 871.310 kb on - strand, within MMJJ_RS04665 at 871.311 kb on + strand, within MMJJ_RS04665 at 871.358 kb on - strand at 871.579 kb on - strand, within MMJJ_RS04670 at 871.580 kb on + strand, within MMJJ_RS04670
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 remove 869,347 - MMJJ_RS04655 0.39 -2.4 869,348 + MMJJ_RS04655 0.39 -1.2 869,349 - +1.1 869,349 - MMJJ_RS04655 0.40 -1.5 869,351 - MMJJ_RS04655 0.40 +0.6 869,451 - +0.1 869,480 + MMJJ_RS04655 0.49 -2.7 869,545 - -0.5 869,551 - MMJJ_RS04655 0.54 -4.0 869,618 + MMJJ_RS04655 0.59 -0.9 869,653 - MMJJ_RS04655 0.61 -2.4 869,653 - MMJJ_RS04655 0.61 -2.6 869,690 + MMJJ_RS04655 0.64 -1.2 869,994 + MMJJ_RS04655 0.85 -0.9 869,994 + MMJJ_RS04655 0.85 -2.1 870,167 - -0.5 870,215 - -0.1 870,228 - +0.7 870,228 - +0.7 870,229 + +0.8 870,299 - MMJJ_RS04660 0.12 -1.5 870,300 + MMJJ_RS04660 0.12 -0.2 870,300 + MMJJ_RS04660 0.12 -0.4 870,310 - MMJJ_RS04660 0.14 -0.2 870,371 - MMJJ_RS04660 0.25 +0.2 870,371 - MMJJ_RS04660 0.25 -0.3 870,380 + MMJJ_RS04660 0.27 +0.7 870,380 + MMJJ_RS04660 0.27 +0.1 870,383 - MMJJ_RS04660 0.27 -0.4 870,384 + MMJJ_RS04660 0.27 +1.9 870,384 + MMJJ_RS04660 0.27 +0.1 870,400 - MMJJ_RS04660 0.30 +0.4 870,403 - MMJJ_RS04660 0.31 -0.1 870,433 - MMJJ_RS04660 0.36 -0.1 870,465 - MMJJ_RS04660 0.42 +1.8 870,466 + MMJJ_RS04660 0.42 -0.1 870,470 - MMJJ_RS04660 0.43 -1.3 870,470 - MMJJ_RS04660 0.43 -1.7 870,470 - MMJJ_RS04660 0.43 +0.6 870,471 + MMJJ_RS04660 0.43 -1.2 870,471 + MMJJ_RS04660 0.43 +1.6 870,471 + MMJJ_RS04660 0.43 +1.0 870,471 + MMJJ_RS04660 0.43 +2.5 870,489 - MMJJ_RS04660 0.46 +0.5 870,490 + MMJJ_RS04660 0.46 +0.7 870,490 + MMJJ_RS04660 0.46 +0.8 870,549 - MMJJ_RS04660 0.57 -0.0 870,549 - MMJJ_RS04660 0.57 -1.2 870,550 + MMJJ_RS04660 0.57 -0.3 870,550 + MMJJ_RS04660 0.57 -0.2 870,553 - MMJJ_RS04660 0.58 +0.2 870,566 - MMJJ_RS04660 0.60 +0.4 870,567 + MMJJ_RS04660 0.60 -0.3 870,599 - MMJJ_RS04660 0.66 +1.0 870,602 + MMJJ_RS04660 0.66 -0.1 870,631 - MMJJ_RS04660 0.72 -0.9 870,660 + MMJJ_RS04660 0.77 +0.3 870,678 + MMJJ_RS04660 0.80 -0.4 870,678 + MMJJ_RS04660 0.80 -0.5 870,733 - -0.5 870,741 + -0.0 870,741 + +0.2 870,746 + +0.2 870,746 + +0.0 870,746 + +0.4 870,781 - -0.2 870,781 - -0.3 870,782 + +0.8 870,830 + +0.5 870,847 + +1.5 870,911 - MMJJ_RS04665 0.18 +0.7 870,971 + MMJJ_RS04665 0.27 -0.3 871,048 - MMJJ_RS04665 0.40 +0.6 871,048 - MMJJ_RS04665 0.40 +0.3 871,049 + MMJJ_RS04665 0.40 -0.3 871,049 + MMJJ_RS04665 0.40 +0.2 871,058 - MMJJ_RS04665 0.42 -0.7 871,066 - MMJJ_RS04665 0.43 -0.5 871,134 + MMJJ_RS04665 0.54 +0.1 871,159 - MMJJ_RS04665 0.58 -0.5 871,182 + MMJJ_RS04665 0.62 -0.1 871,246 - MMJJ_RS04665 0.73 -0.1 871,247 + MMJJ_RS04665 0.73 +0.5 871,247 + MMJJ_RS04665 0.73 -0.5 871,247 + MMJJ_RS04665 0.73 +1.1 871,310 - MMJJ_RS04665 0.83 -0.2 871,310 - MMJJ_RS04665 0.83 +1.1 871,311 + MMJJ_RS04665 0.83 -0.6 871,358 - -0.6 871,579 - MMJJ_RS04670 0.12 -2.2 871,580 + MMJJ_RS04670 0.13 -2.7
Or see this region's nucleotide sequence