Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS04525 and MMJJ_RS04530 are separated by 2 nucleotides MMJJ_RS04530 and MMJJ_RS04535 are separated by 3 nucleotides MMJJ_RS04535 and MMJJ_RS04540 are separated by 21 nucleotides
MMJJ_RS04525: MMJJ_RS04525 - hypothetical protein, at 845,206 to 845,490
_RS04525
MMJJ_RS04530: MMJJ_RS04530 - iron export ABC transporter permease subunit FetB, at 845,493 to 846,239
_RS04530
MMJJ_RS04535: MMJJ_RS04535 - coenzyme gamma-F420-2:alpha-L-glutamate ligase, at 846,243 to 847,100
_RS04535
MMJJ_RS04540: MMJJ_RS04540 - site-2 protease family protein, at 847,122 to 848,249
_RS04540
Position (kb) 846
847
848 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 845.359 kb on + strand, within MMJJ_RS04525 at 845.385 kb on - strand, within MMJJ_RS04525 at 845.468 kb on - strand at 845.513 kb on - strand at 845.514 kb on + strand at 845.525 kb on + strand at 845.533 kb on - strand at 845.561 kb on - strand at 845.562 kb on + strand at 845.585 kb on - strand, within MMJJ_RS04530 at 845.585 kb on - strand, within MMJJ_RS04530 at 845.585 kb on - strand, within MMJJ_RS04530 at 845.585 kb on - strand, within MMJJ_RS04530 at 845.585 kb on - strand, within MMJJ_RS04530 at 845.586 kb on + strand, within MMJJ_RS04530 at 845.586 kb on + strand, within MMJJ_RS04530 at 845.586 kb on + strand, within MMJJ_RS04530 at 845.590 kb on + strand, within MMJJ_RS04530 at 845.611 kb on + strand, within MMJJ_RS04530 at 845.657 kb on - strand, within MMJJ_RS04530 at 845.658 kb on + strand, within MMJJ_RS04530 at 845.689 kb on + strand, within MMJJ_RS04530 at 845.733 kb on + strand, within MMJJ_RS04530 at 845.826 kb on - strand, within MMJJ_RS04530 at 845.828 kb on - strand, within MMJJ_RS04530 at 845.829 kb on + strand, within MMJJ_RS04530 at 845.877 kb on - strand, within MMJJ_RS04530 at 845.878 kb on + strand, within MMJJ_RS04530 at 845.895 kb on - strand, within MMJJ_RS04530 at 845.895 kb on - strand, within MMJJ_RS04530 at 845.895 kb on - strand, within MMJJ_RS04530 at 845.946 kb on + strand, within MMJJ_RS04530 at 845.970 kb on - strand, within MMJJ_RS04530 at 845.970 kb on - strand, within MMJJ_RS04530 at 845.970 kb on - strand, within MMJJ_RS04530 at 845.973 kb on - strand, within MMJJ_RS04530 at 846.039 kb on - strand, within MMJJ_RS04530 at 846.040 kb on + strand, within MMJJ_RS04530 at 846.043 kb on + strand, within MMJJ_RS04530 at 846.043 kb on + strand, within MMJJ_RS04530 at 846.047 kb on - strand, within MMJJ_RS04530 at 846.047 kb on - strand, within MMJJ_RS04530 at 846.048 kb on + strand, within MMJJ_RS04530 at 846.074 kb on - strand, within MMJJ_RS04530 at 846.141 kb on - strand, within MMJJ_RS04530 at 846.142 kb on + strand, within MMJJ_RS04530 at 846.180 kb on - strand at 846.180 kb on - strand at 846.262 kb on + strand at 846.349 kb on - strand, within MMJJ_RS04535 at 846.349 kb on - strand, within MMJJ_RS04535 at 846.350 kb on + strand, within MMJJ_RS04535 at 846.350 kb on + strand, within MMJJ_RS04535 at 846.350 kb on + strand, within MMJJ_RS04535 at 846.352 kb on - strand, within MMJJ_RS04535 at 846.352 kb on - strand, within MMJJ_RS04535 at 846.353 kb on + strand, within MMJJ_RS04535 at 846.389 kb on - strand, within MMJJ_RS04535 at 846.389 kb on - strand, within MMJJ_RS04535 at 846.389 kb on - strand, within MMJJ_RS04535 at 846.390 kb on + strand, within MMJJ_RS04535 at 846.390 kb on + strand, within MMJJ_RS04535 at 846.390 kb on + strand, within MMJJ_RS04535 at 846.444 kb on - strand, within MMJJ_RS04535 at 846.445 kb on + strand, within MMJJ_RS04535 at 846.475 kb on - strand, within MMJJ_RS04535 at 846.475 kb on - strand, within MMJJ_RS04535 at 846.475 kb on - strand, within MMJJ_RS04535 at 846.476 kb on + strand, within MMJJ_RS04535 at 846.477 kb on - strand, within MMJJ_RS04535 at 846.477 kb on - strand, within MMJJ_RS04535 at 846.477 kb on - strand, within MMJJ_RS04535 at 846.477 kb on - strand, within MMJJ_RS04535 at 846.477 kb on - strand, within MMJJ_RS04535 at 846.478 kb on + strand, within MMJJ_RS04535 at 846.478 kb on + strand, within MMJJ_RS04535 at 846.478 kb on + strand, within MMJJ_RS04535 at 846.478 kb on + strand, within MMJJ_RS04535 at 846.494 kb on - strand, within MMJJ_RS04535 at 846.497 kb on - strand, within MMJJ_RS04535 at 846.551 kb on - strand, within MMJJ_RS04535 at 846.559 kb on - strand, within MMJJ_RS04535 at 846.559 kb on - strand, within MMJJ_RS04535 at 846.559 kb on - strand, within MMJJ_RS04535 at 846.559 kb on - strand, within MMJJ_RS04535 at 846.559 kb on - strand, within MMJJ_RS04535 at 846.560 kb on + strand, within MMJJ_RS04535 at 846.560 kb on + strand, within MMJJ_RS04535 at 846.560 kb on + strand, within MMJJ_RS04535 at 846.560 kb on + strand, within MMJJ_RS04535 at 846.560 kb on + strand, within MMJJ_RS04535 at 846.587 kb on + strand, within MMJJ_RS04535 at 846.637 kb on + strand, within MMJJ_RS04535 at 846.699 kb on - strand, within MMJJ_RS04535 at 846.714 kb on - strand, within MMJJ_RS04535 at 846.834 kb on + strand, within MMJJ_RS04535 at 846.888 kb on - strand, within MMJJ_RS04535 at 846.918 kb on + strand, within MMJJ_RS04535 at 846.952 kb on + strand, within MMJJ_RS04535 at 846.952 kb on + strand, within MMJJ_RS04535 at 846.955 kb on + strand, within MMJJ_RS04535 at 846.967 kb on + strand, within MMJJ_RS04535 at 847.017 kb on + strand at 847.020 kb on + strand at 847.060 kb on - strand at 847.060 kb on - strand at 847.060 kb on - strand at 847.061 kb on + strand at 847.062 kb on - strand at 847.063 kb on + strand at 847.063 kb on + strand at 847.096 kb on + strand at 847.136 kb on + strand at 847.149 kb on - strand at 847.150 kb on + strand at 847.152 kb on - strand at 847.153 kb on + strand at 847.164 kb on - strand at 847.164 kb on - strand at 847.170 kb on - strand at 847.170 kb on - strand at 847.171 kb on + strand at 847.171 kb on + strand at 847.171 kb on + strand at 847.172 kb on - strand at 847.172 kb on - strand at 847.172 kb on - strand at 847.172 kb on - strand at 847.173 kb on + strand at 847.173 kb on + strand at 847.173 kb on + strand at 847.173 kb on + strand at 847.173 kb on + strand at 847.199 kb on - strand at 847.203 kb on - strand at 847.321 kb on + strand, within MMJJ_RS04540 at 847.321 kb on + strand, within MMJJ_RS04540 at 847.348 kb on + strand, within MMJJ_RS04540 at 847.350 kb on - strand, within MMJJ_RS04540 at 847.363 kb on - strand, within MMJJ_RS04540 at 847.374 kb on - strand, within MMJJ_RS04540 at 847.375 kb on + strand, within MMJJ_RS04540 at 847.379 kb on - strand, within MMJJ_RS04540 at 847.379 kb on - strand, within MMJJ_RS04540 at 847.381 kb on - strand, within MMJJ_RS04540 at 847.382 kb on + strand, within MMJJ_RS04540 at 847.382 kb on + strand, within MMJJ_RS04540 at 847.382 kb on + strand, within MMJJ_RS04540 at 847.441 kb on + strand, within MMJJ_RS04540 at 847.441 kb on + strand, within MMJJ_RS04540 at 847.444 kb on - strand, within MMJJ_RS04540 at 847.444 kb on - strand, within MMJJ_RS04540 at 847.445 kb on + strand, within MMJJ_RS04540 at 847.445 kb on + strand, within MMJJ_RS04540 at 847.445 kb on + strand, within MMJJ_RS04540 at 847.445 kb on + strand, within MMJJ_RS04540 at 847.445 kb on + strand, within MMJJ_RS04540 at 847.463 kb on - strand, within MMJJ_RS04540 at 847.503 kb on - strand, within MMJJ_RS04540 at 847.503 kb on - strand, within MMJJ_RS04540 at 847.503 kb on - strand, within MMJJ_RS04540 at 847.503 kb on - strand, within MMJJ_RS04540 at 847.503 kb on - strand, within MMJJ_RS04540 at 847.504 kb on + strand at 847.504 kb on + strand, within MMJJ_RS04540 at 847.565 kb on + strand, within MMJJ_RS04540 at 847.569 kb on - strand, within MMJJ_RS04540 at 847.570 kb on + strand, within MMJJ_RS04540 at 847.604 kb on - strand, within MMJJ_RS04540 at 847.655 kb on + strand, within MMJJ_RS04540 at 847.671 kb on - strand at 847.690 kb on + strand, within MMJJ_RS04540 at 847.690 kb on + strand, within MMJJ_RS04540 at 847.767 kb on - strand, within MMJJ_RS04540 at 847.872 kb on + strand, within MMJJ_RS04540 at 847.884 kb on - strand, within MMJJ_RS04540 at 847.885 kb on + strand, within MMJJ_RS04540 at 847.885 kb on + strand, within MMJJ_RS04540 at 847.916 kb on - strand, within MMJJ_RS04540 at 847.917 kb on + strand, within MMJJ_RS04540 at 847.994 kb on - strand, within MMJJ_RS04540 at 847.995 kb on + strand, within MMJJ_RS04540 at 848.064 kb on - strand, within MMJJ_RS04540 at 848.065 kb on + strand, within MMJJ_RS04540 at 848.066 kb on - strand, within MMJJ_RS04540 at 848.067 kb on + strand, within MMJJ_RS04540 at 848.067 kb on + strand, within MMJJ_RS04540 at 848.089 kb on + strand, within MMJJ_RS04540 at 848.092 kb on + strand, within MMJJ_RS04540
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 remove 845,359 + MMJJ_RS04525 0.54 +0.2 845,385 - MMJJ_RS04525 0.63 +0.2 845,468 - +0.5 845,513 - +1.5 845,514 + -0.5 845,525 + +0.3 845,533 - +0.8 845,561 - +0.1 845,562 + -0.2 845,585 - MMJJ_RS04530 0.12 +0.0 845,585 - MMJJ_RS04530 0.12 -0.5 845,585 - MMJJ_RS04530 0.12 -0.1 845,585 - MMJJ_RS04530 0.12 -0.2 845,585 - MMJJ_RS04530 0.12 -0.7 845,586 + MMJJ_RS04530 0.12 -0.0 845,586 + MMJJ_RS04530 0.12 +0.4 845,586 + MMJJ_RS04530 0.12 +0.0 845,590 + MMJJ_RS04530 0.13 +0.5 845,611 + MMJJ_RS04530 0.16 -0.0 845,657 - MMJJ_RS04530 0.22 -0.6 845,658 + MMJJ_RS04530 0.22 -0.1 845,689 + MMJJ_RS04530 0.26 +0.2 845,733 + MMJJ_RS04530 0.32 -0.6 845,826 - MMJJ_RS04530 0.45 -2.5 845,828 - MMJJ_RS04530 0.45 +0.7 845,829 + MMJJ_RS04530 0.45 +0.4 845,877 - MMJJ_RS04530 0.51 -0.4 845,878 + MMJJ_RS04530 0.52 +0.7 845,895 - MMJJ_RS04530 0.54 +0.0 845,895 - MMJJ_RS04530 0.54 -0.4 845,895 - MMJJ_RS04530 0.54 -0.0 845,946 + MMJJ_RS04530 0.61 +1.1 845,970 - MMJJ_RS04530 0.64 -0.3 845,970 - MMJJ_RS04530 0.64 -0.8 845,970 - MMJJ_RS04530 0.64 -0.0 845,973 - MMJJ_RS04530 0.64 +0.1 846,039 - MMJJ_RS04530 0.73 +0.5 846,040 + MMJJ_RS04530 0.73 -0.8 846,043 + MMJJ_RS04530 0.74 +0.6 846,043 + MMJJ_RS04530 0.74 -0.1 846,047 - MMJJ_RS04530 0.74 +0.3 846,047 - MMJJ_RS04530 0.74 +0.5 846,048 + MMJJ_RS04530 0.74 +0.7 846,074 - MMJJ_RS04530 0.78 -0.7 846,141 - MMJJ_RS04530 0.87 -0.2 846,142 + MMJJ_RS04530 0.87 -0.6 846,180 - +0.5 846,180 - -0.2 846,262 + -0.5 846,349 - MMJJ_RS04535 0.12 +0.1 846,349 - MMJJ_RS04535 0.12 -0.7 846,350 + MMJJ_RS04535 0.12 -0.5 846,350 + MMJJ_RS04535 0.12 +0.8 846,350 + MMJJ_RS04535 0.12 -3.0 846,352 - MMJJ_RS04535 0.13 -0.2 846,352 - MMJJ_RS04535 0.13 +0.0 846,353 + MMJJ_RS04535 0.13 -1.3 846,389 - MMJJ_RS04535 0.17 +0.6 846,389 - MMJJ_RS04535 0.17 -1.7 846,389 - MMJJ_RS04535 0.17 +0.4 846,390 + MMJJ_RS04535 0.17 +0.3 846,390 + MMJJ_RS04535 0.17 -0.1 846,390 + MMJJ_RS04535 0.17 -0.1 846,444 - MMJJ_RS04535 0.23 -1.7 846,445 + MMJJ_RS04535 0.24 +0.1 846,475 - MMJJ_RS04535 0.27 -0.0 846,475 - MMJJ_RS04535 0.27 +0.7 846,475 - MMJJ_RS04535 0.27 +0.2 846,476 + MMJJ_RS04535 0.27 +1.7 846,477 - MMJJ_RS04535 0.27 -0.6 846,477 - MMJJ_RS04535 0.27 -0.9 846,477 - MMJJ_RS04535 0.27 +0.7 846,477 - MMJJ_RS04535 0.27 -0.6 846,477 - MMJJ_RS04535 0.27 -1.1 846,478 + MMJJ_RS04535 0.27 -0.0 846,478 + MMJJ_RS04535 0.27 -0.6 846,478 + MMJJ_RS04535 0.27 +0.2 846,478 + MMJJ_RS04535 0.27 -0.7 846,494 - MMJJ_RS04535 0.29 -0.6 846,497 - MMJJ_RS04535 0.30 +0.3 846,551 - MMJJ_RS04535 0.36 -1.9 846,559 - MMJJ_RS04535 0.37 -0.5 846,559 - MMJJ_RS04535 0.37 +3.4 846,559 - MMJJ_RS04535 0.37 -1.5 846,559 - MMJJ_RS04535 0.37 -1.2 846,559 - MMJJ_RS04535 0.37 -0.4 846,560 + MMJJ_RS04535 0.37 -1.1 846,560 + MMJJ_RS04535 0.37 -0.2 846,560 + MMJJ_RS04535 0.37 +0.2 846,560 + MMJJ_RS04535 0.37 +0.8 846,560 + MMJJ_RS04535 0.37 -0.5 846,587 + MMJJ_RS04535 0.40 -0.9 846,637 + MMJJ_RS04535 0.46 -0.7 846,699 - MMJJ_RS04535 0.53 -0.2 846,714 - MMJJ_RS04535 0.55 +0.1 846,834 + MMJJ_RS04535 0.69 -0.4 846,888 - MMJJ_RS04535 0.75 +1.1 846,918 + MMJJ_RS04535 0.79 -1.3 846,952 + MMJJ_RS04535 0.83 -0.3 846,952 + MMJJ_RS04535 0.83 -1.4 846,955 + MMJJ_RS04535 0.83 -0.3 846,967 + MMJJ_RS04535 0.84 -0.5 847,017 + -0.4 847,020 + -0.0 847,060 - -1.1 847,060 - +0.8 847,060 - -0.5 847,061 + -0.2 847,062 - -0.8 847,063 + -0.2 847,063 + -0.9 847,096 + -0.6 847,136 + -0.5 847,149 - +0.2 847,150 + +0.4 847,152 - -0.4 847,153 + +0.1 847,164 - +0.6 847,164 - -1.0 847,170 - +0.6 847,170 - -0.5 847,171 + -0.9 847,171 + +1.7 847,171 + -0.5 847,172 - +0.1 847,172 - -0.7 847,172 - -1.4 847,172 - +0.2 847,173 + -1.0 847,173 + -0.0 847,173 + -1.1 847,173 + -2.2 847,173 + -0.0 847,199 - -0.5 847,203 - -1.2 847,321 + MMJJ_RS04540 0.18 +1.6 847,321 + MMJJ_RS04540 0.18 -0.3 847,348 + MMJJ_RS04540 0.20 +1.5 847,350 - MMJJ_RS04540 0.20 -0.0 847,363 - MMJJ_RS04540 0.21 -0.6 847,374 - MMJJ_RS04540 0.22 -0.6 847,375 + MMJJ_RS04540 0.22 -0.0 847,379 - MMJJ_RS04540 0.23 -0.4 847,379 - MMJJ_RS04540 0.23 -0.4 847,381 - MMJJ_RS04540 0.23 +0.2 847,382 + MMJJ_RS04540 0.23 -0.1 847,382 + MMJJ_RS04540 0.23 +0.4 847,382 + MMJJ_RS04540 0.23 -0.3 847,441 + MMJJ_RS04540 0.28 -1.1 847,441 + MMJJ_RS04540 0.28 +0.2 847,444 - MMJJ_RS04540 0.29 +0.3 847,444 - MMJJ_RS04540 0.29 -0.0 847,445 + MMJJ_RS04540 0.29 -0.2 847,445 + MMJJ_RS04540 0.29 -0.2 847,445 + MMJJ_RS04540 0.29 -0.5 847,445 + MMJJ_RS04540 0.29 -0.6 847,445 + MMJJ_RS04540 0.29 -0.5 847,463 - MMJJ_RS04540 0.30 +1.0 847,503 - MMJJ_RS04540 0.34 -0.2 847,503 - MMJJ_RS04540 0.34 -1.8 847,503 - MMJJ_RS04540 0.34 -1.1 847,503 - MMJJ_RS04540 0.34 -2.9 847,503 - MMJJ_RS04540 0.34 +1.0 847,504 + +0.1 847,504 + MMJJ_RS04540 0.34 -0.1 847,565 + MMJJ_RS04540 0.39 +0.2 847,569 - MMJJ_RS04540 0.40 -0.7 847,570 + MMJJ_RS04540 0.40 -1.3 847,604 - MMJJ_RS04540 0.43 -0.3 847,655 + MMJJ_RS04540 0.47 +0.3 847,671 - -0.5 847,690 + MMJJ_RS04540 0.50 -0.2 847,690 + MMJJ_RS04540 0.50 +0.1 847,767 - MMJJ_RS04540 0.57 -0.2 847,872 + MMJJ_RS04540 0.66 +1.6 847,884 - MMJJ_RS04540 0.68 +1.1 847,885 + MMJJ_RS04540 0.68 -0.3 847,885 + MMJJ_RS04540 0.68 -0.4 847,916 - MMJJ_RS04540 0.70 -0.1 847,917 + MMJJ_RS04540 0.70 +1.2 847,994 - MMJJ_RS04540 0.77 -0.5 847,995 + MMJJ_RS04540 0.77 -0.6 848,064 - MMJJ_RS04540 0.84 -0.7 848,065 + MMJJ_RS04540 0.84 +0.2 848,066 - MMJJ_RS04540 0.84 +0.0 848,067 + MMJJ_RS04540 0.84 +0.5 848,067 + MMJJ_RS04540 0.84 -0.1 848,089 + MMJJ_RS04540 0.86 +0.4 848,092 + MMJJ_RS04540 0.86 -1.0
Or see this region's nucleotide sequence