Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS23840

Experiment: LB, pH5

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS23830 and BPHYT_RS23835 are separated by 73 nucleotidesBPHYT_RS23835 and BPHYT_RS23840 are separated by 161 nucleotidesBPHYT_RS23840 and BPHYT_RS23845 are separated by 672 nucleotides BPHYT_RS23830: BPHYT_RS23830 - acetyl-CoA hydrolase, at 913,909 to 915,423 _RS23830 BPHYT_RS23835: BPHYT_RS23835 - membrane protein, at 915,497 to 915,751 _RS23835 BPHYT_RS23840: BPHYT_RS23840 - methyltransferase, at 915,913 to 916,620 _RS23840 BPHYT_RS23845: BPHYT_RS23845 - hypothetical protein, at 917,293 to 917,817 _RS23845 Position (kb) 915 916 917Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 915.001 kb on + strand, within BPHYT_RS23830at 915.369 kb on + strandat 915.477 kb on + strandat 915.799 kb on + strandat 915.851 kb on + strandat 915.852 kb on - strandat 916.042 kb on - strandat 916.081 kb on - strand, within BPHYT_RS23840at 916.081 kb on - strandat 916.112 kb on + strand, within BPHYT_RS23840at 916.112 kb on + strand, within BPHYT_RS23840at 916.113 kb on - strand, within BPHYT_RS23840at 916.181 kb on + strand, within BPHYT_RS23840at 916.427 kb on + strand, within BPHYT_RS23840at 916.663 kb on + strandat 916.664 kb on - strandat 916.945 kb on - strandat 916.945 kb on - strandat 916.945 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB, pH5
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915,001 + BPHYT_RS23830 0.72 +0.3
915,369 + -0.1
915,477 + -0.1
915,799 + -0.0
915,851 + +0.1
915,852 - +0.4
916,042 - -1.3
916,081 - BPHYT_RS23840 0.24 -3.4
916,081 - -0.9
916,112 + BPHYT_RS23840 0.28 -0.2
916,112 + BPHYT_RS23840 0.28 -3.9
916,113 - BPHYT_RS23840 0.28 -2.4
916,181 + BPHYT_RS23840 0.38 -3.1
916,427 + BPHYT_RS23840 0.73 -4.1
916,663 + +0.5
916,664 - -0.0
916,945 - +0.1
916,945 - +0.6
916,945 - -1.1

Or see this region's nucleotide sequence