Experiment: LB, pH5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS04450 and BPHYT_RS04455 are separated by 12 nucleotides BPHYT_RS04455 and BPHYT_RS04460 are separated by 39 nucleotides BPHYT_RS04460 and BPHYT_RS04465 overlap by 4 nucleotides
BPHYT_RS04450: BPHYT_RS04450 - UDP-glucose 4-epimerase, at 1,029,314 to 1,030,336
_RS04450
BPHYT_RS04455: BPHYT_RS04455 - glycosyl transferase, at 1,030,349 to 1,031,497
_RS04455
BPHYT_RS04460: BPHYT_RS04460 - glycosyl transferase, at 1,031,537 to 1,032,367
_RS04460
BPHYT_RS04465: BPHYT_RS04465 - polysaccharide biosynthesis protein, at 1,032,364 to 1,033,626
_RS04465
Position (kb) 1030
1031
1032 Strain fitness (log2 ratio) -1
0
1 at 1029.528 kb on + strand, within BPHYT_RS04450 at 1029.529 kb on - strand, within BPHYT_RS04450 at 1029.631 kb on - strand, within BPHYT_RS04450 at 1030.028 kb on - strand, within BPHYT_RS04450 at 1030.036 kb on - strand, within BPHYT_RS04450 at 1030.238 kb on + strand at 1030.356 kb on - strand at 1030.852 kb on - strand, within BPHYT_RS04455 at 1031.043 kb on - strand, within BPHYT_RS04455
Per-strain Table
Position Strand Gene LocusTag Fraction LB, pH5 remove 1,029,528 + BPHYT_RS04450 0.21 +0.3 1,029,529 - BPHYT_RS04450 0.21 -0.0 1,029,631 - BPHYT_RS04450 0.31 +0.2 1,030,028 - BPHYT_RS04450 0.70 +0.2 1,030,036 - BPHYT_RS04450 0.71 -0.1 1,030,238 + -1.3 1,030,356 - -0.8 1,030,852 - BPHYT_RS04455 0.44 -0.4 1,031,043 - BPHYT_RS04455 0.60 -1.4
Or see this region's nucleotide sequence