Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS03355

Experiment: LB, pH5

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS03350 and BPHYT_RS03355 are separated by 217 nucleotidesBPHYT_RS03355 and BPHYT_RS03360 are separated by 225 nucleotides BPHYT_RS03350: BPHYT_RS03350 - glycyl-tRNA synthetase subunit alpha, at 753,822 to 754,724 _RS03350 BPHYT_RS03355: BPHYT_RS03355 - glutamate synthase, at 754,942 to 756,552 _RS03355 BPHYT_RS03360: BPHYT_RS03360 - tRNA-specific 2-thiouridylase MnmA, at 756,778 to 757,929 _RS03360 Position (kb) 754 755 756 757Strain fitness (log2 ratio) -3 -2 -1 0 1at 755.058 kb on - strandat 755.415 kb on + strand, within BPHYT_RS03355at 755.421 kb on + strand, within BPHYT_RS03355at 755.421 kb on + strand, within BPHYT_RS03355at 755.422 kb on - strand, within BPHYT_RS03355at 755.924 kb on + strand, within BPHYT_RS03355at 755.973 kb on - strand, within BPHYT_RS03355at 756.260 kb on + strand, within BPHYT_RS03355at 756.261 kb on - strand, within BPHYT_RS03355at 756.321 kb on - strand, within BPHYT_RS03355at 756.659 kb on - strandat 756.659 kb on - strandat 756.720 kb on + strandat 756.720 kb on + strandat 756.720 kb on + strandat 756.720 kb on + strandat 756.721 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB, pH5
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755,058 - -0.3
755,415 + BPHYT_RS03355 0.29 +0.2
755,421 + BPHYT_RS03355 0.30 +0.5
755,421 + BPHYT_RS03355 0.30 +0.0
755,422 - BPHYT_RS03355 0.30 +1.2
755,924 + BPHYT_RS03355 0.61 -0.1
755,973 - BPHYT_RS03355 0.64 -0.1
756,260 + BPHYT_RS03355 0.82 +0.3
756,261 - BPHYT_RS03355 0.82 +1.1
756,321 - BPHYT_RS03355 0.86 -0.1
756,659 - +0.3
756,659 - -0.6
756,720 + +0.6
756,720 + +0.2
756,720 + -0.2
756,720 + -1.1
756,721 - -2.8

Or see this region's nucleotide sequence