Experiment: D-Cycloserine 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMc02818 and SMc02817 overlap by 4 nucleotides SMc02817 and SMc04455 are separated by 34 nucleotides SMc04455 and SMc01573 are separated by 495 nucleotides SMc01573 and SMc01574 are separated by 72 nucleotides
SMc02818: SMc02818 - hypothetical protein, at 2,376,977 to 2,377,921
SMc02818
SMc02817: SMc02817 - hypothetical protein, at 2,377,918 to 2,378,871
SMc02817
SMc04455: SMc04455 - thiamine pyrophosphate protein, at 2,378,906 to 2,380,552
SMc04455
SMc01573: SMc01573 - hypothetical protein, at 2,381,048 to 2,381,386
SMc01573
SMc01574: SMc01574 - hypothetical protein, at 2,381,459 to 2,382,295
SMc01574
Position (kb)
2378
2379
2380
2381 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2377.969 kb on + strand at 2378.033 kb on + strand, within SMc02817 at 2378.033 kb on + strand, within SMc02817 at 2378.125 kb on - strand, within SMc02817 at 2378.282 kb on + strand, within SMc02817 at 2378.324 kb on - strand, within SMc02817 at 2378.326 kb on - strand, within SMc02817 at 2378.600 kb on - strand, within SMc02817 at 2378.602 kb on + strand, within SMc02817 at 2378.602 kb on + strand, within SMc02817 at 2378.602 kb on + strand, within SMc02817 at 2378.603 kb on - strand, within SMc02817 at 2378.653 kb on + strand, within SMc02817 at 2378.653 kb on + strand, within SMc02817 at 2378.653 kb on + strand at 2378.654 kb on - strand, within SMc02817 at 2378.654 kb on - strand, within SMc02817 at 2378.654 kb on - strand, within SMc02817 at 2378.654 kb on - strand, within SMc02817 at 2378.654 kb on - strand, within SMc02817 at 2378.654 kb on - strand, within SMc02817 at 2378.659 kb on + strand, within SMc02817 at 2378.698 kb on + strand, within SMc02817 at 2378.699 kb on - strand, within SMc02817 at 2378.803 kb on + strand at 2378.804 kb on - strand at 2378.804 kb on - strand at 2378.804 kb on - strand at 2378.804 kb on - strand at 2378.804 kb on - strand at 2378.804 kb on - strand at 2378.805 kb on + strand at 2378.844 kb on + strand at 2378.905 kb on + strand at 2378.988 kb on - strand at 2379.012 kb on - strand at 2379.248 kb on + strand, within SMc04455 at 2379.639 kb on - strand, within SMc04455 at 2379.944 kb on + strand, within SMc04455 at 2380.064 kb on + strand, within SMc04455 at 2380.064 kb on + strand, within SMc04455 at 2380.065 kb on - strand, within SMc04455 at 2380.065 kb on - strand, within SMc04455 at 2380.297 kb on + strand, within SMc04455 at 2380.297 kb on + strand, within SMc04455 at 2380.297 kb on + strand, within SMc04455 at 2380.298 kb on - strand, within SMc04455 at 2380.301 kb on + strand, within SMc04455 at 2380.302 kb on - strand, within SMc04455 at 2380.302 kb on - strand, within SMc04455 at 2380.306 kb on + strand, within SMc04455 at 2380.306 kb on + strand, within SMc04455 at 2380.307 kb on - strand at 2380.363 kb on + strand, within SMc04455 at 2380.364 kb on - strand, within SMc04455 at 2380.398 kb on - strand at 2380.550 kb on + strand at 2380.551 kb on - strand at 2380.566 kb on + strand at 2380.765 kb on + strand at 2380.765 kb on + strand at 2380.824 kb on - strand at 2381.077 kb on - strand at 2381.110 kb on - strand, within SMc01573 at 2381.358 kb on + strand at 2381.420 kb on + strand at 2381.485 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.05 mg/ml remove 2,377,969 + -2.6 2,378,033 + SMc02817 0.12 -2.4 2,378,033 + SMc02817 0.12 +0.1 2,378,125 - SMc02817 0.22 -1.6 2,378,282 + SMc02817 0.38 -2.4 2,378,324 - SMc02817 0.43 -0.8 2,378,326 - SMc02817 0.43 -0.9 2,378,600 - SMc02817 0.71 -0.2 2,378,602 + SMc02817 0.72 -0.1 2,378,602 + SMc02817 0.72 -0.3 2,378,602 + SMc02817 0.72 +0.5 2,378,603 - SMc02817 0.72 -0.1 2,378,653 + SMc02817 0.77 +0.2 2,378,653 + SMc02817 0.77 +0.0 2,378,653 + -0.5 2,378,654 - SMc02817 0.77 -0.4 2,378,654 - SMc02817 0.77 +0.2 2,378,654 - SMc02817 0.77 -0.7 2,378,654 - SMc02817 0.77 +0.2 2,378,654 - SMc02817 0.77 -0.6 2,378,654 - SMc02817 0.77 +1.3 2,378,659 + SMc02817 0.78 -0.1 2,378,698 + SMc02817 0.82 +0.7 2,378,699 - SMc02817 0.82 -0.3 2,378,803 + +0.5 2,378,804 - -0.4 2,378,804 - +0.2 2,378,804 - +1.0 2,378,804 - -1.3 2,378,804 - +1.1 2,378,804 - -1.2 2,378,805 + -0.3 2,378,844 + -0.1 2,378,905 + -0.7 2,378,988 - -0.3 2,379,012 - +0.3 2,379,248 + SMc04455 0.21 -0.0 2,379,639 - SMc04455 0.45 -0.8 2,379,944 + SMc04455 0.63 -0.8 2,380,064 + SMc04455 0.70 -2.5 2,380,064 + SMc04455 0.70 -0.2 2,380,065 - SMc04455 0.70 -0.8 2,380,065 - SMc04455 0.70 -1.4 2,380,297 + SMc04455 0.84 +0.5 2,380,297 + SMc04455 0.84 -1.6 2,380,297 + SMc04455 0.84 -0.8 2,380,298 - SMc04455 0.85 +1.3 2,380,301 + SMc04455 0.85 +0.9 2,380,302 - SMc04455 0.85 -0.4 2,380,302 - SMc04455 0.85 -0.5 2,380,306 + SMc04455 0.85 +0.0 2,380,306 + SMc04455 0.85 -1.0 2,380,307 - +3.1 2,380,363 + SMc04455 0.88 +0.0 2,380,364 - SMc04455 0.89 +0.3 2,380,398 - +0.1 2,380,550 + -2.1 2,380,551 - +0.4 2,380,566 + -0.5 2,380,765 + -2.2 2,380,765 + -0.0 2,380,824 - +0.1 2,381,077 - -1.1 2,381,110 - SMc01573 0.18 -0.2 2,381,358 + +0.2 2,381,420 + -0.2 2,381,485 - +0.3
Or see this region's nucleotide sequence