Experiment: D-Cycloserine 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMc02902 and SMc02903 are separated by 6 nucleotides SMc02903 and SMc02904 are separated by 2 nucleotides SMc02904 and SMc02905 overlap by 20 nucleotides
SMc02902: SMc02902 - transcriptional regulator, at 257,736 to 258,428
SMc02902
SMc02903: SMc02903 - hypothetical protein, at 258,435 to 259,415
SMc02903
SMc02904: SMc02904 - hypothetical protein, at 259,418 to 260,143
SMc02904
SMc02905: SMc02905 - DNA polymerase III subunits gamma and tau, at 260,124 to 262,004
SMc02905
Position (kb) 259
260
261 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 258.637 kb on + strand, within SMc02903 at 258.637 kb on + strand, within SMc02903 at 258.689 kb on - strand, within SMc02903 at 258.790 kb on + strand, within SMc02903 at 258.790 kb on + strand, within SMc02903 at 258.848 kb on - strand, within SMc02903 at 258.908 kb on + strand, within SMc02903 at 258.908 kb on + strand, within SMc02903 at 259.052 kb on - strand, within SMc02903 at 259.453 kb on + strand at 259.453 kb on + strand at 259.453 kb on + strand at 259.453 kb on + strand at 259.454 kb on - strand at 259.454 kb on - strand at 259.454 kb on - strand at 259.688 kb on + strand, within SMc02904 at 259.688 kb on + strand, within SMc02904 at 259.689 kb on - strand, within SMc02904 at 259.866 kb on + strand, within SMc02904
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.05 mg/ml remove 258,637 + SMc02903 0.21 -0.2 258,637 + SMc02903 0.21 -1.9 258,689 - SMc02903 0.26 +0.2 258,790 + SMc02903 0.36 +0.7 258,790 + SMc02903 0.36 -0.7 258,848 - SMc02903 0.42 +0.6 258,908 + SMc02903 0.48 -0.1 258,908 + SMc02903 0.48 +0.1 259,052 - SMc02903 0.63 +0.9 259,453 + -0.4 259,453 + -0.2 259,453 + -0.1 259,453 + +1.8 259,454 - -0.7 259,454 - -1.3 259,454 - -0.1 259,688 + SMc02904 0.37 -3.0 259,688 + SMc02904 0.37 +0.2 259,689 - SMc02904 0.37 +2.4 259,866 + SMc02904 0.62 -1.2
Or see this region's nucleotide sequence