Experiment: D-Cycloserine 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMc01090 and SMc01089 are separated by 152 nucleotides SMc01089 and SMc01732 are separated by 2 nucleotides SMc01732 and SMc01731 are separated by 261 nucleotides
SMc01090: SMc01090 - ATP-dependent RNA helicase, at 467,773 to 469,665
SMc01090
SMc01089: SMc01089 - hypothetical protein, at 469,818 to 470,204
SMc01089
SMc01732: SMc01732 - 2,3,4,5-tetrahydropyridine-2,6-carboxylate N-succinyltransferase, at 470,207 to 471,097
SMc01732
SMc01731: SMc01731 - hypothetical protein, at 471,359 to 472,258
SMc01731
Position (kb) 470
471
472 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 469.228 kb on + strand, within SMc01090 at 469.231 kb on - strand, within SMc01090 at 469.252 kb on - strand, within SMc01090 at 469.374 kb on - strand, within SMc01090 at 469.765 kb on + strand at 469.781 kb on + strand at 469.782 kb on - strand at 469.785 kb on + strand at 469.785 kb on + strand at 469.785 kb on + strand at 469.786 kb on - strand at 469.786 kb on - strand at 469.786 kb on - strand at 469.959 kb on - strand, within SMc01089 at 471.103 kb on - strand at 471.109 kb on + strand at 471.109 kb on + strand at 471.109 kb on + strand at 471.109 kb on + strand at 471.110 kb on - strand at 471.142 kb on - strand at 471.142 kb on - strand at 471.142 kb on - strand at 471.263 kb on - strand at 471.274 kb on - strand at 471.285 kb on + strand at 471.285 kb on + strand at 471.485 kb on + strand, within SMc01731 at 471.485 kb on + strand, within SMc01731 at 471.497 kb on + strand, within SMc01731 at 471.498 kb on - strand, within SMc01731 at 471.498 kb on - strand, within SMc01731 at 471.704 kb on + strand, within SMc01731 at 471.704 kb on + strand, within SMc01731 at 471.705 kb on - strand, within SMc01731 at 471.705 kb on - strand, within SMc01731 at 471.705 kb on - strand, within SMc01731 at 471.705 kb on - strand, within SMc01731 at 471.705 kb on - strand, within SMc01731 at 471.708 kb on - strand, within SMc01731 at 471.765 kb on - strand, within SMc01731 at 471.773 kb on + strand, within SMc01731 at 471.773 kb on + strand, within SMc01731 at 471.773 kb on + strand, within SMc01731 at 471.774 kb on - strand, within SMc01731 at 471.774 kb on - strand, within SMc01731 at 471.774 kb on - strand, within SMc01731 at 471.774 kb on - strand, within SMc01731 at 471.774 kb on - strand, within SMc01731
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.05 mg/ml remove 469,228 + SMc01090 0.77 +0.1 469,231 - SMc01090 0.77 -2.7 469,252 - SMc01090 0.78 -1.6 469,374 - SMc01090 0.85 -0.6 469,765 + -0.4 469,781 + +0.4 469,782 - +0.9 469,785 + +1.3 469,785 + -0.0 469,785 + +0.7 469,786 - -0.7 469,786 - -0.2 469,786 - +0.5 469,959 - SMc01089 0.36 +0.2 471,103 - +1.6 471,109 + -2.1 471,109 + -1.7 471,109 + -0.9 471,109 + -0.3 471,110 - -0.2 471,142 - +0.3 471,142 - -0.4 471,142 - +0.2 471,263 - -0.9 471,274 - +1.8 471,285 + -0.1 471,285 + +0.1 471,485 + SMc01731 0.14 +0.2 471,485 + SMc01731 0.14 +0.4 471,497 + SMc01731 0.15 -1.4 471,498 - SMc01731 0.15 +1.5 471,498 - SMc01731 0.15 +2.6 471,704 + SMc01731 0.38 +1.4 471,704 + SMc01731 0.38 +1.0 471,705 - SMc01731 0.38 +0.1 471,705 - SMc01731 0.38 -1.9 471,705 - SMc01731 0.38 +0.0 471,705 - SMc01731 0.38 -0.4 471,705 - SMc01731 0.38 +0.0 471,708 - SMc01731 0.39 +0.5 471,765 - SMc01731 0.45 +1.4 471,773 + SMc01731 0.46 -0.1 471,773 + SMc01731 0.46 +0.5 471,773 + SMc01731 0.46 +0.8 471,774 - SMc01731 0.46 +0.5 471,774 - SMc01731 0.46 +0.2 471,774 - SMc01731 0.46 +0.1 471,774 - SMc01731 0.46 -2.8 471,774 - SMc01731 0.46 +0.7
Or see this region's nucleotide sequence