Experiment: D-Cycloserine 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMc00128 and SMc00457 overlap by 8 nucleotides SMc00457 and SMc00122 are separated by 97 nucleotides SMc00122 and SMc00064 are separated by 120 nucleotides SMc00064 and SMc00123 are separated by 61 nucleotides
SMc00128: SMc00128 - hypothetical protein, at 1,099,690 to 1,100,235
SMc00128
SMc00457: SMc00457 - hypothetical protein, at 1,100,228 to 1,100,815
SMc00457
SMc00122: SMc00122 - penicillin-binding protein, at 1,100,913 to 1,103,147
SMc00122
SMc00064: SMc00064 - hypothetical protein, at 1,103,268 to 1,103,735
SMc00064
SMc00123: SMc00123 - hypothetical protein, at 1,103,797 to 1,104,132
SMc00123
Position (kb)
1100
1101
1102
1103
1104 Strain fitness (log2 ratio)
-1
0
1
2 at 1100.846 kb on - strand at 1100.846 kb on - strand at 1103.154 kb on - strand at 1103.154 kb on - strand at 1103.154 kb on - strand at 1103.154 kb on - strand at 1103.455 kb on - strand, within SMc00064 at 1103.575 kb on - strand, within SMc00064 at 1103.575 kb on - strand, within SMc00064 at 1103.688 kb on + strand, within SMc00064 at 1103.689 kb on - strand at 1103.707 kb on + strand at 1103.708 kb on - strand at 1103.708 kb on - strand at 1103.768 kb on + strand at 1103.769 kb on - strand at 1103.769 kb on - strand at 1104.143 kb on + strand at 1104.143 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.05 mg/ml remove 1,100,846 - -0.3 1,100,846 - -0.8 1,103,154 - +0.7 1,103,154 - +0.0 1,103,154 - +0.2 1,103,154 - +0.2 1,103,455 - SMc00064 0.40 +1.3 1,103,575 - SMc00064 0.66 +1.8 1,103,575 - SMc00064 0.66 +0.9 1,103,688 + SMc00064 0.90 +0.4 1,103,689 - +1.0 1,103,707 + +0.6 1,103,708 - -1.4 1,103,708 - -0.3 1,103,768 + -0.1 1,103,769 - +0.2 1,103,769 - +0.8 1,104,143 + +0.4 1,104,143 + +0.8
Or see this region's nucleotide sequence