Experiment: D-Cycloserine 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa1334 and SMa5030 are separated by 81 nucleotides SMa5030 and SMa1337 are separated by 355 nucleotides
SMa1334: SMa1334 - hypothetical protein, at 729,038 to 730,234
SMa1334
SMa5030: SMa5030 - TetR family transcriptional regulator, at 730,316 to 730,981
SMa5030
SMa1337: SMa1337 - ABC transporter substrate-binding protein, at 731,337 to 732,644
SMa1337
Position (kb) 730
731 Strain fitness (log2 ratio) -2
-1
0
1
2 at 729.413 kb on - strand, within SMa1334 at 729.413 kb on - strand, within SMa1334 at 729.413 kb on - strand, within SMa1334 at 729.549 kb on - strand, within SMa1334 at 729.840 kb on + strand, within SMa1334 at 729.840 kb on + strand, within SMa1334 at 729.840 kb on + strand, within SMa1334 at 729.840 kb on + strand, within SMa1334 at 729.841 kb on - strand, within SMa1334 at 729.841 kb on - strand, within SMa1334 at 729.841 kb on - strand, within SMa1334 at 729.841 kb on - strand, within SMa1334 at 729.841 kb on - strand, within SMa1334 at 729.922 kb on + strand, within SMa1334 at 729.923 kb on - strand, within SMa1334 at 730.179 kb on + strand at 730.180 kb on - strand at 730.221 kb on + strand at 730.221 kb on + strand at 730.222 kb on - strand at 730.225 kb on + strand at 730.226 kb on - strand at 730.226 kb on - strand at 730.360 kb on + strand at 730.360 kb on + strand at 730.450 kb on + strand, within SMa5030 at 730.451 kb on - strand, within SMa5030 at 730.453 kb on - strand, within SMa5030 at 730.514 kb on + strand, within SMa5030 at 730.720 kb on + strand, within SMa5030 at 730.720 kb on + strand, within SMa5030 at 730.721 kb on - strand, within SMa5030 at 730.721 kb on - strand, within SMa5030 at 731.096 kb on - strand at 731.119 kb on + strand at 731.119 kb on + strand at 731.119 kb on + strand at 731.119 kb on + strand at 731.120 kb on - strand at 731.120 kb on - strand at 731.378 kb on + strand at 731.467 kb on + strand at 731.468 kb on - strand, within SMa1337 at 731.476 kb on - strand, within SMa1337 at 731.574 kb on + strand, within SMa1337 at 731.575 kb on - strand, within SMa1337 at 731.631 kb on + strand, within SMa1337 at 731.700 kb on + strand, within SMa1337 at 731.700 kb on + strand, within SMa1337 at 731.703 kb on + strand, within SMa1337 at 731.703 kb on + strand, within SMa1337 at 731.734 kb on - strand, within SMa1337 at 731.829 kb on + strand, within SMa1337 at 731.829 kb on + strand, within SMa1337 at 731.830 kb on - strand, within SMa1337 at 731.830 kb on - strand, within SMa1337 at 731.830 kb on - strand, within SMa1337 at 731.830 kb on - strand, within SMa1337 at 731.928 kb on + strand, within SMa1337
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.05 mg/ml remove 729,413 - SMa1334 0.31 +0.4 729,413 - SMa1334 0.31 +0.6 729,413 - SMa1334 0.31 -0.7 729,549 - SMa1334 0.43 +0.4 729,840 + SMa1334 0.67 +0.1 729,840 + SMa1334 0.67 -1.3 729,840 + SMa1334 0.67 -1.6 729,840 + SMa1334 0.67 +0.9 729,841 - SMa1334 0.67 +1.9 729,841 - SMa1334 0.67 -0.1 729,841 - SMa1334 0.67 +0.0 729,841 - SMa1334 0.67 +0.3 729,841 - SMa1334 0.67 -1.3 729,922 + SMa1334 0.74 +1.9 729,923 - SMa1334 0.74 -0.3 730,179 + -0.0 730,180 - +0.5 730,221 + -0.2 730,221 + -1.2 730,222 - -0.1 730,225 + -0.5 730,226 - +0.3 730,226 - -0.6 730,360 + -0.1 730,360 + +0.4 730,450 + SMa5030 0.20 +1.0 730,451 - SMa5030 0.20 -1.7 730,453 - SMa5030 0.21 -0.9 730,514 + SMa5030 0.30 -0.7 730,720 + SMa5030 0.61 -0.7 730,720 + SMa5030 0.61 +0.8 730,721 - SMa5030 0.61 -0.4 730,721 - SMa5030 0.61 +0.2 731,096 - -0.4 731,119 + -1.4 731,119 + -1.5 731,119 + +0.5 731,119 + +0.2 731,120 - +0.2 731,120 - +0.2 731,378 + +0.3 731,467 + -1.2 731,468 - SMa1337 0.10 +2.0 731,476 - SMa1337 0.11 +0.2 731,574 + SMa1337 0.18 +0.4 731,575 - SMa1337 0.18 +0.1 731,631 + SMa1337 0.22 -0.3 731,700 + SMa1337 0.28 -2.5 731,700 + SMa1337 0.28 -0.4 731,703 + SMa1337 0.28 +0.3 731,703 + SMa1337 0.28 -0.2 731,734 - SMa1337 0.30 +0.0 731,829 + SMa1337 0.38 -0.7 731,829 + SMa1337 0.38 -0.1 731,830 - SMa1337 0.38 -1.1 731,830 - SMa1337 0.38 -0.5 731,830 - SMa1337 0.38 -0.1 731,830 - SMa1337 0.38 +1.2 731,928 + SMa1337 0.45 +0.2
Or see this region's nucleotide sequence