Experiment: D-Cycloserine 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa5022 and SMa5023 overlap by 4 nucleotides SMa5023 and SMa0444 are separated by 83 nucleotides SMa0444 and SMa0445 overlap by 4 nucleotides
SMa5022: SMa5022 - hypothetical protein, at 236,016 to 236,381
SMa5022
SMa5023: SMa5023 - hypothetical protein, at 236,378 to 236,794
SMa5023
SMa0444: SMa0444 - TRm1b transposase, at 236,878 to 237,708
SMa0444
SMa0445: SMa0445 - TRm1a transposase, at 237,705 to 238,097
SMa0445
Position (kb) 236
237 Strain fitness (log2 ratio) -1
0
1
2 at 235.445 kb on - strand at 235.477 kb on + strand at 235.478 kb on - strand at 235.478 kb on - strand at 235.478 kb on - strand at 235.494 kb on + strand at 235.505 kb on - strand at 235.879 kb on + strand at 235.879 kb on + strand at 235.879 kb on + strand at 236.196 kb on - strand, within SMa5022 at 236.215 kb on - strand, within SMa5022 at 236.262 kb on - strand, within SMa5022 at 236.313 kb on + strand, within SMa5022 at 236.313 kb on + strand, within SMa5022 at 236.314 kb on - strand, within SMa5022 at 236.314 kb on - strand, within SMa5022 at 236.316 kb on + strand, within SMa5022 at 236.316 kb on + strand, within SMa5022 at 236.317 kb on - strand, within SMa5022 at 236.366 kb on + strand at 236.366 kb on + strand at 236.367 kb on - strand at 236.433 kb on + strand, within SMa5023 at 236.513 kb on - strand, within SMa5023 at 236.643 kb on + strand, within SMa5023 at 236.644 kb on - strand, within SMa5023 at 236.828 kb on - strand at 236.954 kb on + strand at 236.954 kb on + strand at 236.954 kb on + strand at 236.954 kb on + strand at 236.955 kb on - strand at 236.955 kb on - strand at 237.036 kb on - strand, within SMa0444
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.05 mg/ml remove 235,445 - -0.4 235,477 + +0.8 235,478 - -0.2 235,478 - -1.1 235,478 - +0.1 235,494 + +0.7 235,505 - +0.2 235,879 + +0.7 235,879 + -0.2 235,879 + -0.1 236,196 - SMa5022 0.49 +0.4 236,215 - SMa5022 0.54 +0.3 236,262 - SMa5022 0.67 +1.0 236,313 + SMa5022 0.81 +0.6 236,313 + SMa5022 0.81 -1.0 236,314 - SMa5022 0.81 +0.8 236,314 - SMa5022 0.81 -1.2 236,316 + SMa5022 0.82 -0.5 236,316 + SMa5022 0.82 -1.1 236,317 - SMa5022 0.82 -0.1 236,366 + +0.3 236,366 + -0.6 236,367 - +2.3 236,433 + SMa5023 0.13 -0.2 236,513 - SMa5023 0.32 +2.1 236,643 + SMa5023 0.64 +0.5 236,644 - SMa5023 0.64 +0.4 236,828 - +0.4 236,954 + +1.8 236,954 + -0.4 236,954 + +1.1 236,954 + -0.0 236,955 - +0.3 236,955 - +0.0 237,036 - SMa0444 0.19 -0.2
Or see this region's nucleotide sequence