Experiment: D-Cycloserine 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa2027 and SMa2029 are separated by 154 nucleotides SMa2029 and SMa2031 are separated by 10 nucleotides SMa2031 and SMa2032 overlap by 163 nucleotides SMa2032 and SMa2033 are separated by 532 nucleotides
SMa2027: SMa2027 - LysR family transcriptional regulator, at 1,147,165 to 1,148,058
SMa2027
SMa2029: SMa2029 - amidohydrolase, at 1,148,213 to 1,148,863
SMa2029
SMa2031: SMa2031 - non-heme chloroperoxidase, at 1,148,874 to 1,149,245
SMa2031
SMa2032: SMa2032 - non-heme chloroperoxidase, at 1,149,083 to 1,149,382
SMa2032
SMa2033: SMa2033 - Transglycosylase, at 1,149,915 to 1,150,712
SMa2033
Position (kb)
1148
1149
1150 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1148.041 kb on + strand at 1148.042 kb on - strand at 1148.042 kb on - strand at 1148.042 kb on - strand at 1148.190 kb on - strand at 1148.224 kb on + strand at 1148.288 kb on + strand, within SMa2029 at 1148.289 kb on - strand, within SMa2029 at 1148.323 kb on + strand, within SMa2029 at 1148.359 kb on - strand, within SMa2029 at 1148.359 kb on - strand, within SMa2029 at 1148.442 kb on + strand, within SMa2029 at 1148.480 kb on - strand, within SMa2029 at 1148.813 kb on + strand at 1148.814 kb on - strand at 1148.814 kb on - strand at 1149.121 kb on + strand at 1149.122 kb on - strand at 1149.122 kb on - strand at 1149.466 kb on + strand at 1149.467 kb on - strand at 1149.486 kb on - strand at 1149.500 kb on + strand at 1149.783 kb on - strand at 1149.930 kb on - strand at 1150.110 kb on + strand, within SMa2033 at 1150.110 kb on + strand, within SMa2033 at 1150.111 kb on - strand, within SMa2033 at 1150.118 kb on - strand, within SMa2033 at 1150.178 kb on + strand, within SMa2033
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.05 mg/ml remove 1,148,041 + -0.3 1,148,042 - +0.6 1,148,042 - -0.1 1,148,042 - +3.2 1,148,190 - -1.3 1,148,224 + +0.3 1,148,288 + SMa2029 0.12 +0.4 1,148,289 - SMa2029 0.12 -0.8 1,148,323 + SMa2029 0.17 -0.8 1,148,359 - SMa2029 0.22 +2.8 1,148,359 - SMa2029 0.22 +0.5 1,148,442 + SMa2029 0.35 +0.5 1,148,480 - SMa2029 0.41 -0.2 1,148,813 + +0.4 1,148,814 - +0.8 1,148,814 - +0.6 1,149,121 + +0.6 1,149,122 - -1.1 1,149,122 - +0.2 1,149,466 + -0.4 1,149,467 - -0.2 1,149,486 - +1.2 1,149,500 + -1.7 1,149,783 - -0.7 1,149,930 - -0.6 1,150,110 + SMa2033 0.24 -0.7 1,150,110 + SMa2033 0.24 -0.5 1,150,111 - SMa2033 0.25 -0.2 1,150,118 - SMa2033 0.25 -0.5 1,150,178 + SMa2033 0.33 +0.8
Or see this region's nucleotide sequence