Experiment: D-Cycloserine 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa0601 and SMa0604 are separated by 621 nucleotides SMa0604 and SMa0606 overlap by 4 nucleotides SMa0606 and SMa0607 are separated by 52 nucleotides
SMa0601: SMa0601 - hypothetical protein, at 319,553 to 321,799
SMa0601
SMa0604: SMa0604 - hypothetical protein, at 322,421 to 323,233
SMa0604
SMa0606: SMa0606 - hypothetical protein, at 323,230 to 323,565
SMa0606
SMa0607: SMa0607 - hypothetical protein, at 323,618 to 325,168
SMa0607
Position (kb) 322
323
324 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 321.487 kb on + strand, within SMa0601 at 321.487 kb on + strand, within SMa0601 at 321.488 kb on - strand, within SMa0601 at 321.488 kb on - strand, within SMa0601 at 321.488 kb on - strand, within SMa0601 at 321.513 kb on + strand, within SMa0601 at 321.514 kb on - strand, within SMa0601 at 321.514 kb on - strand, within SMa0601 at 321.517 kb on - strand, within SMa0601 at 321.517 kb on - strand, within SMa0601 at 321.619 kb on + strand at 321.620 kb on - strand at 321.621 kb on + strand at 321.621 kb on + strand at 321.621 kb on + strand at 321.622 kb on - strand at 321.622 kb on - strand at 321.669 kb on + strand at 321.669 kb on + strand at 321.670 kb on - strand at 321.670 kb on - strand at 321.670 kb on - strand at 321.811 kb on - strand at 321.922 kb on + strand at 321.922 kb on + strand at 321.995 kb on + strand at 321.996 kb on - strand at 322.002 kb on + strand at 322.002 kb on + strand at 322.002 kb on + strand at 322.008 kb on - strand at 322.030 kb on - strand at 322.044 kb on + strand at 322.045 kb on - strand at 322.373 kb on - strand at 322.400 kb on + strand at 322.400 kb on + strand at 322.401 kb on - strand at 322.401 kb on - strand at 322.401 kb on - strand at 322.403 kb on + strand at 322.403 kb on + strand at 322.403 kb on + strand at 322.404 kb on - strand at 322.404 kb on - strand at 322.617 kb on - strand, within SMa0604 at 322.621 kb on + strand, within SMa0604 at 322.622 kb on - strand, within SMa0604 at 322.664 kb on + strand, within SMa0604 at 322.732 kb on + strand, within SMa0604 at 322.733 kb on - strand, within SMa0604 at 322.733 kb on - strand, within SMa0604 at 322.776 kb on - strand, within SMa0604 at 322.789 kb on + strand, within SMa0604 at 322.789 kb on + strand, within SMa0604 at 322.789 kb on + strand, within SMa0604 at 322.790 kb on - strand, within SMa0604 at 322.904 kb on + strand, within SMa0604 at 322.905 kb on - strand, within SMa0604 at 323.094 kb on - strand, within SMa0604 at 323.131 kb on + strand, within SMa0604 at 323.153 kb on - strand at 323.258 kb on - strand at 323.444 kb on - strand, within SMa0606 at 323.498 kb on + strand, within SMa0606 at 323.518 kb on + strand, within SMa0606 at 323.519 kb on - strand, within SMa0606 at 323.519 kb on - strand, within SMa0606 at 323.519 kb on - strand, within SMa0606 at 323.519 kb on - strand, within SMa0606 at 323.519 kb on - strand, within SMa0606 at 323.543 kb on - strand at 323.554 kb on + strand at 323.554 kb on + strand at 323.602 kb on - strand at 323.636 kb on + strand at 323.636 kb on + strand at 323.636 kb on + strand at 323.688 kb on - strand at 323.711 kb on + strand at 324.005 kb on + strand, within SMa0607 at 324.006 kb on - strand, within SMa0607 at 324.155 kb on + strand, within SMa0607 at 324.156 kb on - strand, within SMa0607 at 324.200 kb on - strand, within SMa0607 at 324.219 kb on - strand, within SMa0607 at 324.219 kb on - strand, within SMa0607 at 324.221 kb on + strand, within SMa0607 at 324.221 kb on + strand, within SMa0607 at 324.222 kb on - strand, within SMa0607 at 324.222 kb on - strand, within SMa0607
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.05 mg/ml remove 321,487 + SMa0601 0.86 -1.1 321,487 + SMa0601 0.86 +0.3 321,488 - SMa0601 0.86 -0.5 321,488 - SMa0601 0.86 -2.8 321,488 - SMa0601 0.86 -0.5 321,513 + SMa0601 0.87 -0.7 321,514 - SMa0601 0.87 +0.7 321,514 - SMa0601 0.87 +0.4 321,517 - SMa0601 0.87 +0.4 321,517 - SMa0601 0.87 -0.7 321,619 + -0.2 321,620 - -1.4 321,621 + -0.0 321,621 + +0.2 321,621 + -1.6 321,622 - +0.2 321,622 - +0.5 321,669 + -0.0 321,669 + -1.1 321,670 - -0.1 321,670 - -0.2 321,670 - -0.7 321,811 - +0.8 321,922 + +0.0 321,922 + +1.6 321,995 + +0.6 321,996 - +0.3 322,002 + -1.3 322,002 + -0.4 322,002 + +0.4 322,008 - -1.9 322,030 - +1.2 322,044 + -0.1 322,045 - -0.0 322,373 - +0.4 322,400 + -0.9 322,400 + +1.3 322,401 - +2.5 322,401 - +0.1 322,401 - +0.5 322,403 + -0.7 322,403 + -0.1 322,403 + -0.6 322,404 - -1.1 322,404 - -0.5 322,617 - SMa0604 0.24 +0.7 322,621 + SMa0604 0.25 -0.4 322,622 - SMa0604 0.25 +0.1 322,664 + SMa0604 0.30 +0.2 322,732 + SMa0604 0.38 -2.1 322,733 - SMa0604 0.38 -0.8 322,733 - SMa0604 0.38 +0.2 322,776 - SMa0604 0.44 -0.6 322,789 + SMa0604 0.45 -0.6 322,789 + SMa0604 0.45 -0.4 322,789 + SMa0604 0.45 -0.6 322,790 - SMa0604 0.45 +0.3 322,904 + SMa0604 0.59 -1.1 322,905 - SMa0604 0.60 -1.1 323,094 - SMa0604 0.83 -1.7 323,131 + SMa0604 0.87 -0.8 323,153 - -0.7 323,258 - +0.5 323,444 - SMa0606 0.64 +0.7 323,498 + SMa0606 0.80 -0.0 323,518 + SMa0606 0.86 +0.1 323,519 - SMa0606 0.86 +0.7 323,519 - SMa0606 0.86 -0.6 323,519 - SMa0606 0.86 -0.8 323,519 - SMa0606 0.86 -0.8 323,519 - SMa0606 0.86 +1.3 323,543 - -0.7 323,554 + -0.2 323,554 + -0.2 323,602 - +0.3 323,636 + +0.1 323,636 + -1.2 323,636 + +0.1 323,688 - -0.7 323,711 + -0.3 324,005 + SMa0607 0.25 +0.3 324,006 - SMa0607 0.25 -3.6 324,155 + SMa0607 0.35 -2.7 324,156 - SMa0607 0.35 -0.7 324,200 - SMa0607 0.38 -0.4 324,219 - SMa0607 0.39 -0.3 324,219 - SMa0607 0.39 -0.3 324,221 + SMa0607 0.39 +0.6 324,221 + SMa0607 0.39 +0.3 324,222 - SMa0607 0.39 +2.4 324,222 - SMa0607 0.39 -0.0
Or see this region's nucleotide sequence