Experiment: D-Cycloserine 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa0217 and SMa0218 are separated by 9 nucleotides SMa0218 and SMa0220 are separated by 120 nucleotides
SMa0217: SMa0217 - ABC transporter permease, at 118,284 to 119,363
SMa0217
SMa0218: SMa0218 - ABC transporter substrate-binding protein, at 119,373 to 120,323
SMa0218
SMa0220: SMa0220 - NAD-dependent aldehyde dehydrogenase, at 120,444 to 121,958
SMa0220
Position (kb)
119
120
121 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 118.619 kb on + strand, within SMa0217 at 118.825 kb on + strand, within SMa0217 at 118.825 kb on + strand, within SMa0217 at 118.826 kb on - strand, within SMa0217 at 118.904 kb on + strand, within SMa0217 at 118.905 kb on - strand, within SMa0217 at 118.905 kb on - strand, within SMa0217 at 119.017 kb on + strand, within SMa0217 at 119.017 kb on + strand, within SMa0217 at 119.017 kb on + strand, within SMa0217 at 119.017 kb on + strand, within SMa0217 at 119.381 kb on - strand at 119.470 kb on + strand, within SMa0218 at 119.471 kb on - strand, within SMa0218 at 119.502 kb on + strand, within SMa0218 at 119.519 kb on - strand, within SMa0218 at 119.542 kb on + strand, within SMa0218 at 119.542 kb on + strand, within SMa0218 at 119.542 kb on + strand, within SMa0218 at 119.543 kb on - strand, within SMa0218 at 119.568 kb on + strand, within SMa0218 at 119.611 kb on - strand, within SMa0218 at 119.903 kb on - strand, within SMa0218 at 119.932 kb on + strand, within SMa0218 at 119.932 kb on + strand, within SMa0218 at 119.933 kb on - strand, within SMa0218 at 119.933 kb on - strand, within SMa0218 at 119.933 kb on - strand, within SMa0218 at 120.131 kb on - strand, within SMa0218 at 120.220 kb on + strand, within SMa0218 at 120.221 kb on - strand, within SMa0218 at 120.328 kb on + strand at 120.328 kb on + strand at 120.412 kb on + strand at 120.412 kb on + strand at 120.412 kb on + strand at 120.413 kb on - strand at 120.445 kb on + strand at 120.502 kb on + strand at 120.551 kb on + strand at 120.552 kb on + strand at 120.554 kb on - strand at 120.705 kb on + strand, within SMa0220 at 120.814 kb on + strand, within SMa0220 at 120.814 kb on + strand, within SMa0220 at 120.815 kb on - strand, within SMa0220 at 120.817 kb on + strand, within SMa0220 at 120.817 kb on + strand, within SMa0220 at 120.817 kb on + strand, within SMa0220 at 120.817 kb on + strand, within SMa0220 at 120.818 kb on - strand, within SMa0220 at 120.818 kb on - strand, within SMa0220 at 120.892 kb on + strand, within SMa0220 at 120.893 kb on - strand, within SMa0220 at 120.985 kb on - strand, within SMa0220 at 121.075 kb on + strand, within SMa0220 at 121.076 kb on - strand, within SMa0220 at 121.084 kb on + strand, within SMa0220 at 121.285 kb on + strand, within SMa0220 at 121.302 kb on - strand, within SMa0220
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.05 mg/ml remove 118,619 + SMa0217 0.31 -1.1 118,825 + SMa0217 0.50 -0.3 118,825 + SMa0217 0.50 -0.4 118,826 - SMa0217 0.50 +0.5 118,904 + SMa0217 0.57 -0.2 118,905 - SMa0217 0.57 +0.0 118,905 - SMa0217 0.57 -1.5 119,017 + SMa0217 0.68 +0.3 119,017 + SMa0217 0.68 -0.4 119,017 + SMa0217 0.68 +0.6 119,017 + SMa0217 0.68 +2.2 119,381 - -0.4 119,470 + SMa0218 0.10 -1.9 119,471 - SMa0218 0.10 +1.4 119,502 + SMa0218 0.14 +1.5 119,519 - SMa0218 0.15 -0.3 119,542 + SMa0218 0.18 +0.7 119,542 + SMa0218 0.18 +1.1 119,542 + SMa0218 0.18 -1.2 119,543 - SMa0218 0.18 +0.7 119,568 + SMa0218 0.21 +0.9 119,611 - SMa0218 0.25 -0.3 119,903 - SMa0218 0.56 +0.8 119,932 + SMa0218 0.59 -0.0 119,932 + SMa0218 0.59 +0.3 119,933 - SMa0218 0.59 +0.6 119,933 - SMa0218 0.59 +2.3 119,933 - SMa0218 0.59 -0.0 120,131 - SMa0218 0.80 +0.2 120,220 + SMa0218 0.89 -0.1 120,221 - SMa0218 0.89 -1.3 120,328 + -0.3 120,328 + -2.8 120,412 + +0.1 120,412 + +0.8 120,412 + +0.7 120,413 - +2.1 120,445 + +0.2 120,502 + +1.6 120,551 + -2.1 120,552 + +0.6 120,554 - -0.5 120,705 + SMa0220 0.17 +0.8 120,814 + SMa0220 0.24 -0.2 120,814 + SMa0220 0.24 -0.1 120,815 - SMa0220 0.24 -0.1 120,817 + SMa0220 0.25 -0.3 120,817 + SMa0220 0.25 +1.0 120,817 + SMa0220 0.25 +0.4 120,817 + SMa0220 0.25 -0.9 120,818 - SMa0220 0.25 -0.4 120,818 - SMa0220 0.25 +0.3 120,892 + SMa0220 0.30 +0.9 120,893 - SMa0220 0.30 +1.2 120,985 - SMa0220 0.36 -1.6 121,075 + SMa0220 0.42 -1.1 121,076 - SMa0220 0.42 +0.2 121,084 + SMa0220 0.42 -0.1 121,285 + SMa0220 0.56 -1.5 121,302 - SMa0220 0.57 +0.6
Or see this region's nucleotide sequence