Experiment: D-Cycloserine 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b21348 and SM_b21349 overlap by 4 nucleotides SM_b21349 and SM_b21350 are separated by 132 nucleotides SM_b21350 and SM_b21351 are separated by 8 nucleotides
SM_b21348: SM_b21348 - 2-deoxy-D-gluconate 3-dehydrogenase, at 1,014,047 to 1,014,811
_b21348
SM_b21349: SM_b21349 - 5-keto-4-deoxyuronate isomerase, at 1,014,808 to 1,015,656
_b21349
SM_b21350: SM_b21350 - GntR family transcriptional regulator, at 1,015,789 to 1,016,538
_b21350
SM_b21351: SM_b21351 - C4-dicarboxylate large membrane transport protein, at 1,016,547 to 1,017,830
_b21351
Position (kb)
1015
1016
1017 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1014.847 kb on - strand at 1014.847 kb on - strand at 1014.848 kb on + strand at 1014.849 kb on - strand at 1014.855 kb on + strand at 1014.856 kb on - strand at 1014.887 kb on + strand at 1014.887 kb on + strand at 1014.887 kb on + strand at 1014.888 kb on - strand at 1014.955 kb on + strand, within SM_b21349 at 1014.955 kb on + strand, within SM_b21349 at 1014.956 kb on - strand, within SM_b21349 at 1014.956 kb on - strand, within SM_b21349 at 1015.025 kb on + strand, within SM_b21349 at 1015.031 kb on + strand, within SM_b21349 at 1015.031 kb on + strand, within SM_b21349 at 1015.032 kb on - strand, within SM_b21349 at 1015.032 kb on - strand, within SM_b21349 at 1015.222 kb on - strand, within SM_b21349 at 1015.265 kb on + strand, within SM_b21349 at 1015.328 kb on + strand, within SM_b21349 at 1015.329 kb on - strand, within SM_b21349 at 1015.437 kb on + strand, within SM_b21349 at 1015.518 kb on + strand, within SM_b21349 at 1015.520 kb on + strand, within SM_b21349 at 1015.520 kb on + strand, within SM_b21349 at 1015.520 kb on + strand, within SM_b21349 at 1015.521 kb on - strand, within SM_b21349 at 1015.634 kb on + strand at 1015.696 kb on + strand at 1015.696 kb on + strand at 1015.697 kb on - strand at 1015.697 kb on - strand at 1015.700 kb on + strand at 1015.701 kb on - strand at 1015.701 kb on - strand at 1015.724 kb on + strand at 1015.725 kb on - strand at 1015.759 kb on - strand at 1016.051 kb on + strand, within SM_b21350 at 1016.052 kb on - strand, within SM_b21350 at 1016.052 kb on - strand, within SM_b21350 at 1016.054 kb on + strand, within SM_b21350 at 1016.181 kb on + strand, within SM_b21350 at 1016.455 kb on + strand, within SM_b21350 at 1016.455 kb on + strand, within SM_b21350 at 1016.456 kb on - strand, within SM_b21350 at 1016.575 kb on - strand at 1016.593 kb on + strand at 1016.594 kb on - strand at 1016.594 kb on - strand at 1016.594 kb on - strand at 1016.640 kb on + strand at 1016.640 kb on + strand at 1016.641 kb on - strand at 1016.641 kb on - strand at 1016.739 kb on + strand, within SM_b21351 at 1016.739 kb on + strand, within SM_b21351 at 1016.779 kb on + strand, within SM_b21351 at 1016.878 kb on - strand, within SM_b21351 at 1016.878 kb on - strand, within SM_b21351 at 1016.883 kb on + strand, within SM_b21351 at 1016.884 kb on - strand, within SM_b21351 at 1016.884 kb on - strand, within SM_b21351 at 1016.884 kb on - strand, within SM_b21351 at 1017.088 kb on + strand, within SM_b21351 at 1017.088 kb on + strand, within SM_b21351 at 1017.089 kb on - strand, within SM_b21351 at 1017.138 kb on + strand, within SM_b21351 at 1017.138 kb on + strand, within SM_b21351 at 1017.194 kb on - strand, within SM_b21351 at 1017.194 kb on - strand, within SM_b21351 at 1017.259 kb on + strand, within SM_b21351 at 1017.286 kb on + strand, within SM_b21351 at 1017.359 kb on - strand, within SM_b21351 at 1017.359 kb on - strand, within SM_b21351 at 1017.443 kb on - strand, within SM_b21351 at 1017.480 kb on + strand, within SM_b21351 at 1017.481 kb on - strand, within SM_b21351
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.05 mg/ml remove 1,014,847 - +1.0 1,014,847 - +0.9 1,014,848 + +0.1 1,014,849 - +0.8 1,014,855 + +1.0 1,014,856 - +0.6 1,014,887 + -0.1 1,014,887 + +0.1 1,014,887 + -0.7 1,014,888 - +0.3 1,014,955 + SM_b21349 0.17 +0.6 1,014,955 + SM_b21349 0.17 -0.3 1,014,956 - SM_b21349 0.17 +0.9 1,014,956 - SM_b21349 0.17 -1.2 1,015,025 + SM_b21349 0.26 +0.6 1,015,031 + SM_b21349 0.26 +0.0 1,015,031 + SM_b21349 0.26 -0.2 1,015,032 - SM_b21349 0.26 +0.1 1,015,032 - SM_b21349 0.26 -1.8 1,015,222 - SM_b21349 0.49 +0.1 1,015,265 + SM_b21349 0.54 -0.6 1,015,328 + SM_b21349 0.61 +0.7 1,015,329 - SM_b21349 0.61 +0.1 1,015,437 + SM_b21349 0.74 -0.4 1,015,518 + SM_b21349 0.84 +0.4 1,015,520 + SM_b21349 0.84 -2.1 1,015,520 + SM_b21349 0.84 +0.2 1,015,520 + SM_b21349 0.84 -0.8 1,015,521 - SM_b21349 0.84 +0.5 1,015,634 + +2.4 1,015,696 + -0.0 1,015,696 + -0.6 1,015,697 - +1.2 1,015,697 - +0.5 1,015,700 + +1.4 1,015,701 - -0.4 1,015,701 - -0.7 1,015,724 + -1.3 1,015,725 - +0.1 1,015,759 - -0.6 1,016,051 + SM_b21350 0.35 -0.0 1,016,052 - SM_b21350 0.35 +0.6 1,016,052 - SM_b21350 0.35 -0.2 1,016,054 + SM_b21350 0.35 -1.1 1,016,181 + SM_b21350 0.52 -0.6 1,016,455 + SM_b21350 0.89 +0.3 1,016,455 + SM_b21350 0.89 -1.0 1,016,456 - SM_b21350 0.89 -2.5 1,016,575 - -0.9 1,016,593 + +0.7 1,016,594 - -0.3 1,016,594 - +0.3 1,016,594 - +0.8 1,016,640 + +0.2 1,016,640 + -0.0 1,016,641 - -0.1 1,016,641 - -1.9 1,016,739 + SM_b21351 0.15 +0.1 1,016,739 + SM_b21351 0.15 +1.1 1,016,779 + SM_b21351 0.18 +0.8 1,016,878 - SM_b21351 0.26 +0.6 1,016,878 - SM_b21351 0.26 +0.2 1,016,883 + SM_b21351 0.26 -0.0 1,016,884 - SM_b21351 0.26 +0.6 1,016,884 - SM_b21351 0.26 +0.8 1,016,884 - SM_b21351 0.26 -0.6 1,017,088 + SM_b21351 0.42 -0.2 1,017,088 + SM_b21351 0.42 -1.0 1,017,089 - SM_b21351 0.42 +0.7 1,017,138 + SM_b21351 0.46 -0.5 1,017,138 + SM_b21351 0.46 +0.2 1,017,194 - SM_b21351 0.50 +0.3 1,017,194 - SM_b21351 0.50 +0.5 1,017,259 + SM_b21351 0.55 +0.1 1,017,286 + SM_b21351 0.58 -1.0 1,017,359 - SM_b21351 0.63 -0.6 1,017,359 - SM_b21351 0.63 +1.9 1,017,443 - SM_b21351 0.70 +2.6 1,017,480 + SM_b21351 0.73 +0.6 1,017,481 - SM_b21351 0.73 +0.3
Or see this region's nucleotide sequence