Experiment: D-Cycloserine 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b21245 and SM_b21246 are separated by 62 nucleotides SM_b21246 and SM_b21247 are separated by 40 nucleotides SM_b21247 and SM_b21248 are separated by 68 nucleotides
SM_b21245: SM_b21245 - OMA family outer membrane protein, at 813,621 to 814,871
_b21245
SM_b21246: SM_b21246 - UDP-phosphate galactose phosphotransferase, at 814,934 to 815,626
_b21246
SM_b21247: SM_b21247 - hypothetical protein, at 815,667 to 816,353
_b21247
SM_b21248: SM_b21248 - aminotransferase, at 816,422 to 817,612
_b21248
Position (kb) 815
816
817 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 814.702 kb on + strand, within SM_b21245 at 814.703 kb on - strand, within SM_b21245 at 814.703 kb on - strand at 814.709 kb on + strand, within SM_b21245 at 814.709 kb on + strand, within SM_b21245 at 814.710 kb on - strand, within SM_b21245 at 814.756 kb on - strand at 814.761 kb on + strand at 814.761 kb on + strand at 814.761 kb on + strand at 814.762 kb on - strand at 814.762 kb on - strand at 814.762 kb on - strand at 814.804 kb on + strand at 814.804 kb on + strand at 814.869 kb on + strand at 814.869 kb on + strand at 814.869 kb on + strand at 814.870 kb on - strand at 814.870 kb on - strand at 814.904 kb on + strand at 814.904 kb on + strand at 814.905 kb on - strand at 814.928 kb on - strand at 814.928 kb on - strand at 814.945 kb on + strand at 814.945 kb on + strand at 814.995 kb on - strand at 815.149 kb on + strand, within SM_b21246 at 815.310 kb on + strand, within SM_b21246 at 815.310 kb on + strand, within SM_b21246 at 815.311 kb on - strand, within SM_b21246 at 815.311 kb on - strand, within SM_b21246 at 815.358 kb on + strand, within SM_b21246 at 815.409 kb on + strand, within SM_b21246 at 815.473 kb on + strand, within SM_b21246 at 815.473 kb on + strand, within SM_b21246 at 815.473 kb on + strand, within SM_b21246 at 815.474 kb on - strand, within SM_b21246 at 815.474 kb on - strand, within SM_b21246 at 815.474 kb on - strand, within SM_b21246 at 815.683 kb on + strand at 815.684 kb on - strand at 815.897 kb on - strand, within SM_b21247 at 816.106 kb on - strand, within SM_b21247 at 816.141 kb on + strand, within SM_b21247 at 816.141 kb on + strand, within SM_b21247 at 816.142 kb on - strand, within SM_b21247 at 816.142 kb on - strand, within SM_b21247 at 816.142 kb on - strand, within SM_b21247 at 816.142 kb on - strand, within SM_b21247 at 816.179 kb on + strand, within SM_b21247 at 816.351 kb on + strand at 816.352 kb on - strand at 816.386 kb on + strand at 816.387 kb on - strand at 816.387 kb on - strand at 816.401 kb on + strand at 816.440 kb on + strand at 816.536 kb on + strand at 816.536 kb on + strand at 816.536 kb on + strand at 816.537 kb on - strand at 816.537 kb on - strand at 816.537 kb on - strand at 816.537 kb on - strand at 816.537 kb on - strand at 816.555 kb on + strand, within SM_b21248 at 816.754 kb on - strand, within SM_b21248 at 816.832 kb on - strand, within SM_b21248 at 816.867 kb on + strand, within SM_b21248 at 816.867 kb on + strand, within SM_b21248 at 817.055 kb on + strand, within SM_b21248 at 817.055 kb on + strand, within SM_b21248 at 817.056 kb on - strand, within SM_b21248 at 817.056 kb on - strand, within SM_b21248 at 817.068 kb on - strand, within SM_b21248 at 817.194 kb on + strand, within SM_b21248 at 817.194 kb on + strand, within SM_b21248 at 817.194 kb on + strand, within SM_b21248 at 817.194 kb on + strand, within SM_b21248 at 817.194 kb on + strand, within SM_b21248 at 817.195 kb on - strand, within SM_b21248 at 817.195 kb on - strand, within SM_b21248 at 817.213 kb on + strand, within SM_b21248 at 817.213 kb on + strand, within SM_b21248 at 817.216 kb on + strand, within SM_b21248 at 817.216 kb on + strand, within SM_b21248 at 817.298 kb on + strand, within SM_b21248 at 817.299 kb on - strand, within SM_b21248 at 817.299 kb on - strand, within SM_b21248 at 817.301 kb on - strand, within SM_b21248 at 817.301 kb on - strand, within SM_b21248
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.05 mg/ml remove 814,702 + SM_b21245 0.86 -0.5 814,703 - SM_b21245 0.86 -0.1 814,703 - -0.5 814,709 + SM_b21245 0.87 -0.2 814,709 + SM_b21245 0.87 -0.9 814,710 - SM_b21245 0.87 -0.1 814,756 - -1.0 814,761 + -2.2 814,761 + -0.8 814,761 + -0.7 814,762 - +0.2 814,762 - +0.8 814,762 - -2.2 814,804 + -0.8 814,804 + +0.2 814,869 + +1.1 814,869 + +0.4 814,869 + +0.9 814,870 - -0.2 814,870 - +0.9 814,904 + -3.0 814,904 + -1.1 814,905 - -0.3 814,928 - +1.0 814,928 - -0.7 814,945 + +0.2 814,945 + -0.6 814,995 - +0.2 815,149 + SM_b21246 0.31 -0.0 815,310 + SM_b21246 0.54 -0.1 815,310 + SM_b21246 0.54 +0.7 815,311 - SM_b21246 0.54 -1.0 815,311 - SM_b21246 0.54 +0.5 815,358 + SM_b21246 0.61 -0.3 815,409 + SM_b21246 0.69 -0.8 815,473 + SM_b21246 0.78 -1.1 815,473 + SM_b21246 0.78 -0.6 815,473 + SM_b21246 0.78 -1.3 815,474 - SM_b21246 0.78 +0.3 815,474 - SM_b21246 0.78 +0.8 815,474 - SM_b21246 0.78 +1.1 815,683 + +0.7 815,684 - -0.0 815,897 - SM_b21247 0.33 -0.7 816,106 - SM_b21247 0.64 -0.9 816,141 + SM_b21247 0.69 +0.1 816,141 + SM_b21247 0.69 -1.3 816,142 - SM_b21247 0.69 -0.2 816,142 - SM_b21247 0.69 -2.1 816,142 - SM_b21247 0.69 +2.1 816,142 - SM_b21247 0.69 +0.3 816,179 + SM_b21247 0.75 -0.6 816,351 + -0.6 816,352 - -0.5 816,386 + +0.1 816,387 - +0.4 816,387 - -0.1 816,401 + -0.0 816,440 + -0.2 816,536 + -0.8 816,536 + -0.1 816,536 + +0.1 816,537 - -0.8 816,537 - +0.9 816,537 - -0.0 816,537 - -2.5 816,537 - +0.4 816,555 + SM_b21248 0.11 +0.2 816,754 - SM_b21248 0.28 +0.4 816,832 - SM_b21248 0.34 +0.1 816,867 + SM_b21248 0.37 +1.0 816,867 + SM_b21248 0.37 +0.1 817,055 + SM_b21248 0.53 -0.3 817,055 + SM_b21248 0.53 +0.8 817,056 - SM_b21248 0.53 -0.6 817,056 - SM_b21248 0.53 -0.1 817,068 - SM_b21248 0.54 -0.5 817,194 + SM_b21248 0.65 +1.0 817,194 + SM_b21248 0.65 +0.8 817,194 + SM_b21248 0.65 -1.2 817,194 + SM_b21248 0.65 +0.6 817,194 + SM_b21248 0.65 +0.2 817,195 - SM_b21248 0.65 -0.6 817,195 - SM_b21248 0.65 -0.2 817,213 + SM_b21248 0.66 +0.4 817,213 + SM_b21248 0.66 -0.4 817,216 + SM_b21248 0.67 -0.6 817,216 + SM_b21248 0.67 -0.4 817,298 + SM_b21248 0.74 +0.6 817,299 - SM_b21248 0.74 +0.2 817,299 - SM_b21248 0.74 -1.8 817,301 - SM_b21248 0.74 +0.3 817,301 - SM_b21248 0.74 -0.2
Or see this region's nucleotide sequence