Experiment: D-Cycloserine 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b20981 and SM_b20982 are separated by 77 nucleotides SM_b20982 and SM_b20983 are separated by 45 nucleotides SM_b20983 and SM_b20984 overlap by 16 nucleotides SM_b20984 and SM_b20985 are separated by 3 nucleotides SM_b20985 and SM_b20986 are separated by 2 nucleotides
SM_b20981: SM_b20981 - C4-dicarboxylate transporter substrate-binding protein precursor, at 1,213,597 to 1,214,574
_b20981
SM_b20982: SM_b20982 - hypothetical protein, at 1,214,652 to 1,214,912
_b20982
SM_b20983: SM_b20983 - hypothetical protein, at 1,214,958 to 1,215,320
_b20983
SM_b20984: SM_b20984 - Nitrite reductase (NAD(P)H) (EC 1.7.1.4) (from data) , at 1,215,305 to 1,217,767
_b20984
SM_b20985: SM_b20985 - nitrite reductase [NAD(P)H], small subunit protein, at 1,217,771 to 1,218,109
_b20985
SM_b20986: SM_b20986 - Nitrate reductase (EC 1.7.99.4) (from data) , at 1,218,112 to 1,220,769
_b20986
Position (kb)
1215
1216
1217
1218 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1214.438 kb on + strand, within SM_b20981 at 1214.438 kb on + strand, within SM_b20981 at 1214.438 kb on + strand, within SM_b20981 at 1214.438 kb on + strand, within SM_b20981 at 1214.505 kb on - strand at 1214.587 kb on - strand at 1214.587 kb on - strand at 1214.974 kb on - strand at 1215.109 kb on + strand, within SM_b20983 at 1215.268 kb on - strand, within SM_b20983 at 1215.268 kb on - strand, within SM_b20983 at 1215.284 kb on + strand at 1215.285 kb on - strand at 1215.440 kb on + strand at 1215.440 kb on + strand at 1215.440 kb on + strand at 1215.485 kb on + strand at 1215.485 kb on + strand at 1215.518 kb on + strand at 1215.518 kb on + strand at 1215.519 kb on - strand at 1215.542 kb on + strand at 1215.542 kb on + strand at 1215.543 kb on - strand at 1215.543 kb on - strand at 1215.544 kb on + strand at 1215.545 kb on - strand at 1215.545 kb on - strand at 1215.617 kb on + strand, within SM_b20984 at 1215.618 kb on - strand, within SM_b20984 at 1215.698 kb on + strand, within SM_b20984 at 1215.698 kb on + strand, within SM_b20984 at 1215.699 kb on - strand, within SM_b20984 at 1215.699 kb on - strand, within SM_b20984 at 1215.699 kb on - strand, within SM_b20984 at 1215.884 kb on + strand, within SM_b20984 at 1215.884 kb on + strand, within SM_b20984 at 1215.884 kb on + strand, within SM_b20984 at 1215.885 kb on - strand, within SM_b20984 at 1216.169 kb on + strand, within SM_b20984 at 1216.169 kb on + strand, within SM_b20984 at 1216.295 kb on + strand, within SM_b20984 at 1216.367 kb on + strand, within SM_b20984 at 1216.367 kb on + strand, within SM_b20984 at 1216.368 kb on - strand, within SM_b20984 at 1216.368 kb on - strand, within SM_b20984 at 1216.415 kb on + strand, within SM_b20984 at 1216.416 kb on - strand, within SM_b20984 at 1216.495 kb on + strand, within SM_b20984 at 1216.495 kb on + strand, within SM_b20984 at 1216.538 kb on - strand, within SM_b20984 at 1216.739 kb on + strand, within SM_b20984 at 1216.739 kb on + strand, within SM_b20984 at 1216.740 kb on - strand, within SM_b20984 at 1216.740 kb on - strand, within SM_b20984 at 1216.904 kb on + strand, within SM_b20984 at 1216.904 kb on + strand, within SM_b20984 at 1216.905 kb on - strand, within SM_b20984 at 1216.908 kb on - strand, within SM_b20984 at 1216.946 kb on + strand, within SM_b20984 at 1216.946 kb on + strand, within SM_b20984 at 1216.947 kb on - strand, within SM_b20984 at 1217.003 kb on + strand, within SM_b20984 at 1217.003 kb on + strand, within SM_b20984 at 1217.003 kb on + strand, within SM_b20984 at 1217.003 kb on + strand, within SM_b20984 at 1217.003 kb on + strand, within SM_b20984 at 1217.003 kb on + strand, within SM_b20984 at 1217.003 kb on + strand, within SM_b20984 at 1217.004 kb on - strand, within SM_b20984 at 1217.509 kb on + strand, within SM_b20984 at 1217.509 kb on + strand, within SM_b20984 at 1217.510 kb on - strand, within SM_b20984 at 1217.531 kb on + strand at 1217.531 kb on + strand at 1217.532 kb on - strand at 1217.532 kb on - strand at 1217.532 kb on - strand at 1217.564 kb on + strand at 1217.765 kb on + strand at 1218.108 kb on - strand at 1218.143 kb on - strand at 1218.403 kb on + strand, within SM_b20986 at 1218.403 kb on + strand, within SM_b20986 at 1218.403 kb on + strand, within SM_b20986 at 1218.403 kb on + strand, within SM_b20986 at 1218.404 kb on - strand, within SM_b20986 at 1218.404 kb on - strand, within SM_b20986 at 1218.404 kb on - strand, within SM_b20986 at 1218.433 kb on + strand, within SM_b20986 at 1218.434 kb on - strand, within SM_b20986 at 1218.434 kb on - strand, within SM_b20986 at 1218.434 kb on - strand, within SM_b20986 at 1218.434 kb on - strand, within SM_b20986 at 1218.434 kb on - strand, within SM_b20986 at 1218.436 kb on + strand, within SM_b20986 at 1218.436 kb on + strand, within SM_b20986 at 1218.436 kb on + strand, within SM_b20986 at 1218.436 kb on + strand, within SM_b20986 at 1218.436 kb on + strand, within SM_b20986 at 1218.436 kb on + strand, within SM_b20986 at 1218.437 kb on - strand, within SM_b20986 at 1218.481 kb on - strand, within SM_b20986 at 1218.565 kb on + strand, within SM_b20986 at 1218.638 kb on - strand, within SM_b20986
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.05 mg/ml remove 1,214,438 + SM_b20981 0.86 -0.9 1,214,438 + SM_b20981 0.86 +0.0 1,214,438 + SM_b20981 0.86 +1.4 1,214,438 + SM_b20981 0.86 +0.6 1,214,505 - -1.9 1,214,587 - -0.6 1,214,587 - +0.8 1,214,974 - -1.1 1,215,109 + SM_b20983 0.42 -0.3 1,215,268 - SM_b20983 0.85 -0.4 1,215,268 - SM_b20983 0.85 +0.4 1,215,284 + +1.2 1,215,285 - +1.2 1,215,440 + -0.1 1,215,440 + +0.4 1,215,440 + -0.7 1,215,485 + -0.0 1,215,485 + -1.8 1,215,518 + -0.3 1,215,518 + -0.7 1,215,519 - +0.3 1,215,542 + -1.3 1,215,542 + -0.5 1,215,543 - +0.0 1,215,543 - -1.3 1,215,544 + -1.5 1,215,545 - -0.8 1,215,545 - -0.4 1,215,617 + SM_b20984 0.13 -0.3 1,215,618 - SM_b20984 0.13 -0.0 1,215,698 + SM_b20984 0.16 -0.2 1,215,698 + SM_b20984 0.16 -0.3 1,215,699 - SM_b20984 0.16 -0.8 1,215,699 - SM_b20984 0.16 +0.4 1,215,699 - SM_b20984 0.16 -0.2 1,215,884 + SM_b20984 0.24 -0.5 1,215,884 + SM_b20984 0.24 +0.9 1,215,884 + SM_b20984 0.24 -0.7 1,215,885 - SM_b20984 0.24 -0.2 1,216,169 + SM_b20984 0.35 -1.7 1,216,169 + SM_b20984 0.35 -0.2 1,216,295 + SM_b20984 0.40 +0.0 1,216,367 + SM_b20984 0.43 +1.6 1,216,367 + SM_b20984 0.43 -0.3 1,216,368 - SM_b20984 0.43 -1.4 1,216,368 - SM_b20984 0.43 -2.5 1,216,415 + SM_b20984 0.45 -0.1 1,216,416 - SM_b20984 0.45 +0.2 1,216,495 + SM_b20984 0.48 -0.2 1,216,495 + SM_b20984 0.48 -0.5 1,216,538 - SM_b20984 0.50 -0.6 1,216,739 + SM_b20984 0.58 -2.2 1,216,739 + SM_b20984 0.58 +0.7 1,216,740 - SM_b20984 0.58 +0.3 1,216,740 - SM_b20984 0.58 +1.2 1,216,904 + SM_b20984 0.65 -1.0 1,216,904 + SM_b20984 0.65 -0.6 1,216,905 - SM_b20984 0.65 -0.8 1,216,908 - SM_b20984 0.65 -0.1 1,216,946 + SM_b20984 0.67 +0.4 1,216,946 + SM_b20984 0.67 -1.9 1,216,947 - SM_b20984 0.67 +0.3 1,217,003 + SM_b20984 0.69 +0.1 1,217,003 + SM_b20984 0.69 -0.4 1,217,003 + SM_b20984 0.69 +0.4 1,217,003 + SM_b20984 0.69 -0.0 1,217,003 + SM_b20984 0.69 +0.1 1,217,003 + SM_b20984 0.69 -0.7 1,217,003 + SM_b20984 0.69 -0.2 1,217,004 - SM_b20984 0.69 -0.2 1,217,509 + SM_b20984 0.89 -1.3 1,217,509 + SM_b20984 0.89 +0.0 1,217,510 - SM_b20984 0.90 -1.0 1,217,531 + -0.8 1,217,531 + +0.3 1,217,532 - -0.0 1,217,532 - +0.8 1,217,532 - +1.2 1,217,564 + +0.4 1,217,765 + +0.2 1,218,108 - -0.7 1,218,143 - -0.7 1,218,403 + SM_b20986 0.11 -0.2 1,218,403 + SM_b20986 0.11 +4.6 1,218,403 + SM_b20986 0.11 +0.8 1,218,403 + SM_b20986 0.11 +0.7 1,218,404 - SM_b20986 0.11 -0.4 1,218,404 - SM_b20986 0.11 -0.2 1,218,404 - SM_b20986 0.11 +0.2 1,218,433 + SM_b20986 0.12 +3.1 1,218,434 - SM_b20986 0.12 -0.2 1,218,434 - SM_b20986 0.12 -0.2 1,218,434 - SM_b20986 0.12 -0.3 1,218,434 - SM_b20986 0.12 +0.9 1,218,434 - SM_b20986 0.12 +0.4 1,218,436 + SM_b20986 0.12 +0.2 1,218,436 + SM_b20986 0.12 -0.2 1,218,436 + SM_b20986 0.12 +0.0 1,218,436 + SM_b20986 0.12 -0.1 1,218,436 + SM_b20986 0.12 +0.9 1,218,436 + SM_b20986 0.12 +0.8 1,218,437 - SM_b20986 0.12 -0.3 1,218,481 - SM_b20986 0.14 -0.6 1,218,565 + SM_b20986 0.17 -0.7 1,218,638 - SM_b20986 0.20 +0.6
Or see this region's nucleotide sequence