Experiment: D-Cycloserine 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b20300 and SM_b20301 overlap by 4 nucleotides SM_b20301 and SM_b20302 are separated by 147 nucleotides SM_b20302 and SM_b20303 are separated by 196 nucleotides SM_b20303 and SM_b20304 are separated by 56 nucleotides
SM_b20300: SM_b20300 - adenylate cyclase, at 306,932 to 308,845
_b20300
SM_b20301: SM_b20301 - dehydgrogenase, at 308,842 to 309,972
_b20301
SM_b20302: SM_b20302 - hypothetical protein, at 310,120 to 310,281
_b20302
SM_b20303: SM_b20303 - hypothetical protein, at 310,478 to 310,705
_b20303
SM_b20304: SM_b20304 - ISRm2011-2 transposase, C-terminal portion, at 310,762 to 311,358
_b20304
Position (kb) 308
309
310 Strain fitness (log2 ratio) -2
-1
0
1
2 at 307.935 kb on + strand, within SM_b20300 at 307.935 kb on + strand, within SM_b20300 at 307.936 kb on - strand, within SM_b20300 at 307.936 kb on - strand, within SM_b20300 at 307.988 kb on + strand, within SM_b20300 at 307.988 kb on + strand, within SM_b20300 at 307.988 kb on + strand, within SM_b20300 at 307.988 kb on + strand, within SM_b20300 at 308.048 kb on + strand, within SM_b20300 at 308.048 kb on + strand, within SM_b20300 at 308.048 kb on + strand, within SM_b20300 at 308.079 kb on - strand, within SM_b20300 at 308.079 kb on - strand, within SM_b20300 at 308.192 kb on + strand, within SM_b20300 at 308.192 kb on + strand, within SM_b20300 at 308.193 kb on - strand, within SM_b20300 at 308.193 kb on - strand, within SM_b20300 at 308.193 kb on - strand, within SM_b20300 at 308.193 kb on - strand, within SM_b20300 at 308.507 kb on + strand, within SM_b20300 at 308.508 kb on - strand, within SM_b20300 at 308.510 kb on + strand, within SM_b20300 at 308.510 kb on + strand, within SM_b20300 at 308.510 kb on + strand, within SM_b20300 at 308.511 kb on - strand, within SM_b20300 at 308.511 kb on - strand, within SM_b20300 at 308.512 kb on + strand, within SM_b20300 at 308.512 kb on + strand, within SM_b20300 at 308.512 kb on + strand, within SM_b20300 at 308.512 kb on + strand, within SM_b20300 at 308.513 kb on - strand, within SM_b20300 at 308.513 kb on - strand, within SM_b20300 at 308.513 kb on - strand, within SM_b20300 at 308.516 kb on + strand, within SM_b20300 at 308.516 kb on + strand, within SM_b20300 at 308.516 kb on + strand, within SM_b20300 at 308.516 kb on + strand, within SM_b20300 at 308.538 kb on - strand, within SM_b20300 at 309.001 kb on - strand, within SM_b20301 at 309.007 kb on + strand, within SM_b20301 at 309.064 kb on - strand, within SM_b20301 at 309.065 kb on + strand, within SM_b20301 at 309.065 kb on + strand, within SM_b20301 at 309.065 kb on + strand, within SM_b20301 at 309.066 kb on - strand, within SM_b20301 at 309.066 kb on - strand, within SM_b20301 at 309.105 kb on + strand, within SM_b20301 at 309.162 kb on + strand, within SM_b20301 at 309.163 kb on - strand, within SM_b20301 at 309.164 kb on + strand, within SM_b20301 at 309.197 kb on + strand, within SM_b20301 at 309.218 kb on - strand, within SM_b20301 at 309.314 kb on + strand, within SM_b20301 at 309.315 kb on - strand, within SM_b20301 at 309.315 kb on - strand, within SM_b20301 at 309.463 kb on + strand, within SM_b20301 at 309.463 kb on + strand, within SM_b20301 at 309.463 kb on + strand, within SM_b20301 at 309.464 kb on - strand, within SM_b20301 at 309.638 kb on + strand, within SM_b20301 at 309.639 kb on - strand, within SM_b20301 at 309.889 kb on - strand at 310.021 kb on + strand at 310.227 kb on - strand, within SM_b20302 at 310.237 kb on + strand, within SM_b20302 at 310.238 kb on - strand, within SM_b20302 at 310.238 kb on - strand, within SM_b20302 at 310.318 kb on - strand at 310.373 kb on - strand at 310.433 kb on + strand at 310.462 kb on + strand at 310.548 kb on - strand, within SM_b20303 at 310.548 kb on - strand, within SM_b20303 at 310.622 kb on + strand, within SM_b20303 at 310.623 kb on - strand, within SM_b20303 at 310.704 kb on - strand at 310.727 kb on - strand at 310.756 kb on - strand at 310.756 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.05 mg/ml remove 307,935 + SM_b20300 0.52 +0.1 307,935 + SM_b20300 0.52 -0.2 307,936 - SM_b20300 0.52 -1.4 307,936 - SM_b20300 0.52 -0.2 307,988 + SM_b20300 0.55 +0.5 307,988 + SM_b20300 0.55 -0.6 307,988 + SM_b20300 0.55 -0.1 307,988 + SM_b20300 0.55 +0.2 308,048 + SM_b20300 0.58 +0.8 308,048 + SM_b20300 0.58 +0.3 308,048 + SM_b20300 0.58 +0.1 308,079 - SM_b20300 0.60 -0.8 308,079 - SM_b20300 0.60 -0.1 308,192 + SM_b20300 0.66 +1.7 308,192 + SM_b20300 0.66 -1.3 308,193 - SM_b20300 0.66 -0.3 308,193 - SM_b20300 0.66 +0.6 308,193 - SM_b20300 0.66 +0.5 308,193 - SM_b20300 0.66 +0.4 308,507 + SM_b20300 0.82 +0.1 308,508 - SM_b20300 0.82 -0.3 308,510 + SM_b20300 0.82 +0.9 308,510 + SM_b20300 0.82 +0.7 308,510 + SM_b20300 0.82 -1.2 308,511 - SM_b20300 0.82 -0.3 308,511 - SM_b20300 0.82 -0.1 308,512 + SM_b20300 0.83 +0.2 308,512 + SM_b20300 0.83 -0.1 308,512 + SM_b20300 0.83 -0.5 308,512 + SM_b20300 0.83 +0.3 308,513 - SM_b20300 0.83 -0.6 308,513 - SM_b20300 0.83 +0.2 308,513 - SM_b20300 0.83 -1.1 308,516 + SM_b20300 0.83 -0.4 308,516 + SM_b20300 0.83 +0.4 308,516 + SM_b20300 0.83 +1.0 308,516 + SM_b20300 0.83 +0.0 308,538 - SM_b20300 0.84 -0.8 309,001 - SM_b20301 0.14 -1.6 309,007 + SM_b20301 0.15 -0.2 309,064 - SM_b20301 0.20 +1.5 309,065 + SM_b20301 0.20 +0.4 309,065 + SM_b20301 0.20 -0.3 309,065 + SM_b20301 0.20 +0.8 309,066 - SM_b20301 0.20 -0.0 309,066 - SM_b20301 0.20 +0.5 309,105 + SM_b20301 0.23 -0.1 309,162 + SM_b20301 0.28 -1.7 309,163 - SM_b20301 0.28 -0.9 309,164 + SM_b20301 0.28 +1.2 309,197 + SM_b20301 0.31 -0.9 309,218 - SM_b20301 0.33 -1.0 309,314 + SM_b20301 0.42 -1.6 309,315 - SM_b20301 0.42 -0.0 309,315 - SM_b20301 0.42 +0.1 309,463 + SM_b20301 0.55 -0.7 309,463 + SM_b20301 0.55 -0.2 309,463 + SM_b20301 0.55 +0.7 309,464 - SM_b20301 0.55 -0.1 309,638 + SM_b20301 0.70 -0.2 309,639 - SM_b20301 0.70 -1.1 309,889 - -0.3 310,021 + -0.4 310,227 - SM_b20302 0.66 +1.0 310,237 + SM_b20302 0.72 +1.1 310,238 - SM_b20302 0.73 +0.1 310,238 - SM_b20302 0.73 -0.9 310,318 - +0.3 310,373 - -0.1 310,433 + -1.5 310,462 + -2.0 310,548 - SM_b20303 0.31 -0.3 310,548 - SM_b20303 0.31 -0.6 310,622 + SM_b20303 0.63 +0.4 310,623 - SM_b20303 0.64 +1.1 310,704 - +2.0 310,727 - -0.6 310,756 - +0.5 310,756 - -0.1
Or see this region's nucleotide sequence