Experiment: LB, pH4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS21330 and BPHYT_RS21335 are separated by 246 nucleotides BPHYT_RS21335 and BPHYT_RS21340 overlap by 4 nucleotides BPHYT_RS21340 and BPHYT_RS21345 overlap by 4 nucleotides
BPHYT_RS21330: BPHYT_RS21330 - hypothetical protein, at 345,059 to 345,493
_RS21330
BPHYT_RS21335: BPHYT_RS21335 - CDP-alcohol phosphatidyltransferase, at 345,740 to 346,441
_RS21335
BPHYT_RS21340: BPHYT_RS21340 - 2OG-Fe(II) oxygenase, at 346,438 to 347,250
_RS21340
BPHYT_RS21345: BPHYT_RS21345 - membrane protein, at 347,247 to 348,266
_RS21345
Position (kb) 346
347
348 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 345.494 kb on - strand at 345.521 kb on + strand at 345.521 kb on + strand at 345.522 kb on - strand at 345.612 kb on + strand at 345.612 kb on + strand at 345.612 kb on + strand at 345.654 kb on - strand at 345.724 kb on + strand at 345.724 kb on + strand at 345.724 kb on + strand at 345.727 kb on - strand at 345.727 kb on - strand at 345.728 kb on + strand at 345.728 kb on + strand at 345.728 kb on + strand at 345.970 kb on + strand, within BPHYT_RS21335 at 346.443 kb on + strand at 346.482 kb on - strand at 346.482 kb on - strand at 346.725 kb on + strand, within BPHYT_RS21340 at 346.726 kb on - strand, within BPHYT_RS21340 at 346.855 kb on + strand, within BPHYT_RS21340 at 346.858 kb on + strand, within BPHYT_RS21340 at 346.858 kb on + strand, within BPHYT_RS21340 at 346.859 kb on - strand, within BPHYT_RS21340 at 346.899 kb on + strand, within BPHYT_RS21340 at 346.927 kb on - strand, within BPHYT_RS21340
Per-strain Table
Position Strand Gene LocusTag Fraction LB, pH4 remove 345,494 - -0.4 345,521 + -0.3 345,521 + -0.6 345,522 - -0.2 345,612 + -0.7 345,612 + -1.7 345,612 + -0.8 345,654 - -2.6 345,724 + +0.3 345,724 + -0.8 345,724 + +0.1 345,727 - -1.1 345,727 - -2.6 345,728 + -0.4 345,728 + -0.8 345,728 + -1.0 345,970 + BPHYT_RS21335 0.33 -2.9 346,443 + -3.1 346,482 - -0.7 346,482 - -1.7 346,725 + BPHYT_RS21340 0.35 -2.7 346,726 - BPHYT_RS21340 0.35 -3.0 346,855 + BPHYT_RS21340 0.51 -3.5 346,858 + BPHYT_RS21340 0.52 -0.5 346,858 + BPHYT_RS21340 0.52 -2.1 346,859 - BPHYT_RS21340 0.52 -2.4 346,899 + BPHYT_RS21340 0.57 -1.0 346,927 - BPHYT_RS21340 0.60 -2.0
Or see this region's nucleotide sequence