Experiment: CDM1 without Glycine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS12525 and BBR_RS12530 overlap by 1 nucleotides BBR_RS12530 and BBR_RS20715 are separated by 10 nucleotides BBR_RS20715 and BBR_RS12535 are separated by 86 nucleotides
BBR_RS12525: BBR_RS12525 - aldo/keto reductase, at 565,924 to 566,793
_RS12525
BBR_RS12530: BBR_RS12530 - HNH endonuclease, at 566,793 to 567,551
_RS12530
BBR_RS20715: BBR_RS20715 - hypothetical protein, at 567,562 to 567,885
_RS20715
BBR_RS12535: BBR_RS12535 - divalent metal cation transporter MntH, at 567,972 to 569,297
_RS12535
Position (kb) 567
568 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5
6 at 566.566 kb on + strand, within BBR_RS12525 at 566.566 kb on + strand, within BBR_RS12525 at 566.574 kb on - strand, within BBR_RS12525 at 566.574 kb on - strand, within BBR_RS12525 at 566.597 kb on + strand, within BBR_RS12525 at 566.597 kb on + strand, within BBR_RS12525 at 566.647 kb on + strand, within BBR_RS12525 at 566.684 kb on + strand, within BBR_RS12525 at 566.692 kb on - strand, within BBR_RS12525 at 566.692 kb on - strand, within BBR_RS12525 at 566.694 kb on + strand, within BBR_RS12525 at 566.699 kb on + strand, within BBR_RS12525 at 566.721 kb on + strand at 566.745 kb on - strand at 566.745 kb on - strand at 566.746 kb on + strand at 566.762 kb on + strand at 566.769 kb on - strand at 566.778 kb on - strand at 566.782 kb on + strand at 566.784 kb on - strand at 566.784 kb on - strand at 566.787 kb on + strand at 566.796 kb on + strand at 566.804 kb on + strand at 566.804 kb on + strand at 566.804 kb on - strand at 566.851 kb on + strand at 566.859 kb on - strand at 566.860 kb on - strand at 566.861 kb on + strand at 566.880 kb on - strand, within BBR_RS12530 at 566.898 kb on + strand, within BBR_RS12530 at 566.906 kb on - strand, within BBR_RS12530 at 566.933 kb on + strand, within BBR_RS12530 at 566.949 kb on + strand, within BBR_RS12530 at 566.954 kb on + strand, within BBR_RS12530 at 566.975 kb on - strand, within BBR_RS12530 at 567.007 kb on - strand, within BBR_RS12530 at 567.012 kb on + strand, within BBR_RS12530 at 567.030 kb on + strand, within BBR_RS12530 at 567.162 kb on + strand, within BBR_RS12530 at 567.173 kb on - strand, within BBR_RS12530 at 567.175 kb on + strand, within BBR_RS12530 at 567.179 kb on + strand, within BBR_RS12530 at 567.185 kb on + strand, within BBR_RS12530 at 567.185 kb on + strand, within BBR_RS12530 at 567.185 kb on + strand, within BBR_RS12530 at 567.185 kb on + strand, within BBR_RS12530 at 567.185 kb on + strand, within BBR_RS12530 at 567.185 kb on + strand, within BBR_RS12530 at 567.185 kb on + strand, within BBR_RS12530 at 567.185 kb on + strand, within BBR_RS12530 at 567.185 kb on + strand, within BBR_RS12530 at 567.185 kb on + strand, within BBR_RS12530 at 567.185 kb on + strand, within BBR_RS12530 at 567.185 kb on + strand, within BBR_RS12530 at 567.185 kb on + strand, within BBR_RS12530 at 567.193 kb on - strand, within BBR_RS12530 at 567.193 kb on - strand, within BBR_RS12530 at 567.193 kb on - strand, within BBR_RS12530 at 567.193 kb on - strand, within BBR_RS12530 at 567.193 kb on - strand, within BBR_RS12530 at 567.193 kb on - strand, within BBR_RS12530 at 567.193 kb on - strand, within BBR_RS12530 at 567.193 kb on - strand, within BBR_RS12530 at 567.193 kb on - strand, within BBR_RS12530 at 567.193 kb on - strand, within BBR_RS12530 at 567.203 kb on - strand, within BBR_RS12530 at 567.235 kb on + strand, within BBR_RS12530 at 567.240 kb on - strand, within BBR_RS12530 at 567.296 kb on - strand, within BBR_RS12530 at 567.324 kb on + strand, within BBR_RS12530 at 567.334 kb on + strand, within BBR_RS12530 at 567.334 kb on + strand, within BBR_RS12530 at 567.349 kb on + strand, within BBR_RS12530 at 567.357 kb on - strand, within BBR_RS12530 at 567.386 kb on - strand, within BBR_RS12530 at 567.393 kb on + strand, within BBR_RS12530 at 567.393 kb on + strand, within BBR_RS12530 at 567.423 kb on - strand, within BBR_RS12530 at 567.513 kb on + strand at 567.513 kb on + strand at 567.518 kb on - strand at 567.520 kb on - strand at 567.522 kb on + strand at 567.546 kb on + strand at 567.546 kb on + strand at 567.546 kb on + strand at 567.554 kb on - strand at 567.559 kb on + strand at 567.562 kb on - strand at 567.562 kb on - strand at 567.580 kb on + strand at 567.585 kb on - strand at 567.590 kb on - strand at 567.592 kb on - strand at 567.651 kb on + strand, within BBR_RS20715 at 567.661 kb on - strand, within BBR_RS20715 at 567.663 kb on - strand, within BBR_RS20715 at 567.670 kb on + strand, within BBR_RS20715 at 567.723 kb on + strand, within BBR_RS20715 at 567.731 kb on - strand, within BBR_RS20715 at 567.731 kb on - strand, within BBR_RS20715 at 567.731 kb on - strand, within BBR_RS20715 at 567.731 kb on - strand, within BBR_RS20715 at 567.918 kb on + strand at 567.929 kb on + strand at 567.937 kb on - strand at 567.958 kb on - strand at 567.968 kb on + strand at 567.976 kb on - strand at 567.989 kb on - strand at 568.147 kb on - strand, within BBR_RS12535 at 568.151 kb on - strand, within BBR_RS12535 at 568.196 kb on - strand, within BBR_RS12535 at 568.202 kb on + strand, within BBR_RS12535 at 568.203 kb on - strand, within BBR_RS12535 at 568.205 kb on + strand, within BBR_RS12535 at 568.210 kb on + strand, within BBR_RS12535 at 568.210 kb on + strand, within BBR_RS12535 at 568.223 kb on + strand, within BBR_RS12535 at 568.246 kb on + strand, within BBR_RS12535 at 568.246 kb on + strand, within BBR_RS12535 at 568.254 kb on - strand, within BBR_RS12535 at 568.254 kb on - strand, within BBR_RS12535 at 568.280 kb on + strand, within BBR_RS12535 at 568.296 kb on + strand, within BBR_RS12535 at 568.319 kb on - strand, within BBR_RS12535 at 568.339 kb on + strand, within BBR_RS12535 at 568.349 kb on + strand, within BBR_RS12535 at 568.356 kb on - strand, within BBR_RS12535 at 568.357 kb on - strand, within BBR_RS12535 at 568.357 kb on - strand, within BBR_RS12535 at 568.357 kb on - strand, within BBR_RS12535 at 568.423 kb on - strand, within BBR_RS12535 at 568.475 kb on - strand, within BBR_RS12535 at 568.481 kb on + strand, within BBR_RS12535 at 568.481 kb on + strand, within BBR_RS12535 at 568.502 kb on + strand, within BBR_RS12535 at 568.502 kb on + strand, within BBR_RS12535 at 568.504 kb on + strand, within BBR_RS12535 at 568.515 kb on - strand, within BBR_RS12535 at 568.515 kb on - strand, within BBR_RS12535 at 568.547 kb on - strand, within BBR_RS12535 at 568.568 kb on + strand, within BBR_RS12535 at 568.576 kb on - strand, within BBR_RS12535 at 568.576 kb on - strand, within BBR_RS12535 at 568.604 kb on - strand, within BBR_RS12535 at 568.619 kb on + strand, within BBR_RS12535 at 568.619 kb on + strand, within BBR_RS12535 at 568.634 kb on - strand, within BBR_RS12535 at 568.638 kb on + strand, within BBR_RS12535 at 568.648 kb on - strand, within BBR_RS12535 at 568.692 kb on - strand, within BBR_RS12535 at 568.696 kb on + strand, within BBR_RS12535 at 568.714 kb on + strand, within BBR_RS12535 at 568.716 kb on + strand, within BBR_RS12535 at 568.717 kb on + strand, within BBR_RS12535 at 568.736 kb on + strand, within BBR_RS12535 at 568.762 kb on - strand, within BBR_RS12535 at 568.762 kb on - strand, within BBR_RS12535 at 568.786 kb on - strand, within BBR_RS12535 at 568.827 kb on - strand, within BBR_RS12535 at 568.842 kb on + strand, within BBR_RS12535
Per-strain Table
Position Strand Gene LocusTag Fraction CDM1 without Glycine remove 566,566 + BBR_RS12525 0.74 -2.1 566,566 + BBR_RS12525 0.74 -0.5 566,574 - BBR_RS12525 0.75 +1.0 566,574 - BBR_RS12525 0.75 +1.4 566,597 + BBR_RS12525 0.77 +2.7 566,597 + BBR_RS12525 0.77 +0.7 566,647 + BBR_RS12525 0.83 +2.7 566,684 + BBR_RS12525 0.87 -0.9 566,692 - BBR_RS12525 0.88 -2.1 566,692 - BBR_RS12525 0.88 -2.7 566,694 + BBR_RS12525 0.89 -1.3 566,699 + BBR_RS12525 0.89 -4.1 566,721 + -1.3 566,745 - +2.5 566,745 - +2.6 566,746 + -1.5 566,762 + +1.3 566,769 - -0.7 566,778 - +3.1 566,782 + -2.0 566,784 - +0.6 566,784 - -2.1 566,787 + -0.1 566,796 + +4.1 566,804 + -2.5 566,804 + +2.3 566,804 - +3.1 566,851 + +1.2 566,859 - +1.8 566,860 - +3.5 566,861 + -1.9 566,880 - BBR_RS12530 0.11 -1.8 566,898 + BBR_RS12530 0.14 +1.5 566,906 - BBR_RS12530 0.15 +3.9 566,933 + BBR_RS12530 0.18 -1.3 566,949 + BBR_RS12530 0.21 -2.4 566,954 + BBR_RS12530 0.21 -1.2 566,975 - BBR_RS12530 0.24 +0.9 567,007 - BBR_RS12530 0.28 -1.4 567,012 + BBR_RS12530 0.29 -2.6 567,030 + BBR_RS12530 0.31 -1.4 567,162 + BBR_RS12530 0.49 +4.0 567,173 - BBR_RS12530 0.50 +0.7 567,175 + BBR_RS12530 0.50 +0.2 567,179 + BBR_RS12530 0.51 -3.7 567,185 + BBR_RS12530 0.52 +5.9 567,185 + BBR_RS12530 0.52 -1.8 567,185 + BBR_RS12530 0.52 -2.8 567,185 + BBR_RS12530 0.52 +4.0 567,185 + BBR_RS12530 0.52 +2.8 567,185 + BBR_RS12530 0.52 -1.5 567,185 + BBR_RS12530 0.52 -1.9 567,185 + BBR_RS12530 0.52 +3.6 567,185 + BBR_RS12530 0.52 +1.7 567,185 + BBR_RS12530 0.52 -2.4 567,185 + BBR_RS12530 0.52 +1.2 567,185 + BBR_RS12530 0.52 -1.4 567,185 + BBR_RS12530 0.52 -1.9 567,193 - BBR_RS12530 0.53 -1.3 567,193 - BBR_RS12530 0.53 +3.5 567,193 - BBR_RS12530 0.53 +2.0 567,193 - BBR_RS12530 0.53 +4.3 567,193 - BBR_RS12530 0.53 +2.0 567,193 - BBR_RS12530 0.53 +0.7 567,193 - BBR_RS12530 0.53 -1.2 567,193 - BBR_RS12530 0.53 -0.7 567,193 - BBR_RS12530 0.53 -0.7 567,193 - BBR_RS12530 0.53 +0.8 567,203 - BBR_RS12530 0.54 +1.8 567,235 + BBR_RS12530 0.58 -1.1 567,240 - BBR_RS12530 0.59 +2.9 567,296 - BBR_RS12530 0.66 +0.5 567,324 + BBR_RS12530 0.70 +0.8 567,334 + BBR_RS12530 0.71 -0.3 567,334 + BBR_RS12530 0.71 +2.1 567,349 + BBR_RS12530 0.73 -0.6 567,357 - BBR_RS12530 0.74 -0.7 567,386 - BBR_RS12530 0.78 -1.6 567,393 + BBR_RS12530 0.79 -2.3 567,393 + BBR_RS12530 0.79 +1.9 567,423 - BBR_RS12530 0.83 +1.5 567,513 + -1.6 567,513 + +2.3 567,518 - +3.8 567,520 - -0.6 567,522 + +0.9 567,546 + -2.2 567,546 + +4.1 567,546 + -1.3 567,554 - -0.8 567,559 + +1.5 567,562 - -1.1 567,562 - +3.1 567,580 + -0.7 567,585 - -1.1 567,590 - -1.6 567,592 - +0.0 567,651 + BBR_RS20715 0.27 +1.0 567,661 - BBR_RS20715 0.31 +2.4 567,663 - BBR_RS20715 0.31 -3.0 567,670 + BBR_RS20715 0.33 -1.7 567,723 + BBR_RS20715 0.50 -1.9 567,731 - BBR_RS20715 0.52 -1.3 567,731 - BBR_RS20715 0.52 +0.5 567,731 - BBR_RS20715 0.52 -1.1 567,731 - BBR_RS20715 0.52 -1.4 567,918 + -0.5 567,929 + -0.5 567,937 - -1.9 567,958 - -1.0 567,968 + -2.4 567,976 - +3.1 567,989 - +3.4 568,147 - BBR_RS12535 0.13 -1.8 568,151 - BBR_RS12535 0.13 +1.1 568,196 - BBR_RS12535 0.17 +1.7 568,202 + BBR_RS12535 0.17 +3.6 568,203 - BBR_RS12535 0.17 -1.7 568,205 + BBR_RS12535 0.18 -0.1 568,210 + BBR_RS12535 0.18 -1.6 568,210 + BBR_RS12535 0.18 +3.7 568,223 + BBR_RS12535 0.19 -2.7 568,246 + BBR_RS12535 0.21 +2.8 568,246 + BBR_RS12535 0.21 +3.4 568,254 - BBR_RS12535 0.21 +0.7 568,254 - BBR_RS12535 0.21 -1.2 568,280 + BBR_RS12535 0.23 +1.4 568,296 + BBR_RS12535 0.24 +2.5 568,319 - BBR_RS12535 0.26 -1.5 568,339 + BBR_RS12535 0.28 +1.6 568,349 + BBR_RS12535 0.28 +0.3 568,356 - BBR_RS12535 0.29 -2.5 568,357 - BBR_RS12535 0.29 +2.4 568,357 - BBR_RS12535 0.29 -0.0 568,357 - BBR_RS12535 0.29 +0.2 568,423 - BBR_RS12535 0.34 +1.9 568,475 - BBR_RS12535 0.38 -1.5 568,481 + BBR_RS12535 0.38 +3.5 568,481 + BBR_RS12535 0.38 +0.7 568,502 + BBR_RS12535 0.40 +0.5 568,502 + BBR_RS12535 0.40 +2.0 568,504 + BBR_RS12535 0.40 +2.3 568,515 - BBR_RS12535 0.41 -1.2 568,515 - BBR_RS12535 0.41 +1.9 568,547 - BBR_RS12535 0.43 -1.5 568,568 + BBR_RS12535 0.45 -1.8 568,576 - BBR_RS12535 0.46 +2.6 568,576 - BBR_RS12535 0.46 +2.8 568,604 - BBR_RS12535 0.48 +5.5 568,619 + BBR_RS12535 0.49 -2.7 568,619 + BBR_RS12535 0.49 +4.5 568,634 - BBR_RS12535 0.50 +0.3 568,638 + BBR_RS12535 0.50 -0.1 568,648 - BBR_RS12535 0.51 -1.6 568,692 - BBR_RS12535 0.54 -0.9 568,696 + BBR_RS12535 0.55 +1.5 568,714 + BBR_RS12535 0.56 -1.0 568,716 + BBR_RS12535 0.56 +2.1 568,717 + BBR_RS12535 0.56 -1.4 568,736 + BBR_RS12535 0.58 +0.6 568,762 - BBR_RS12535 0.60 -3.6 568,762 - BBR_RS12535 0.60 -0.9 568,786 - BBR_RS12535 0.61 -1.9 568,827 - BBR_RS12535 0.64 -2.0 568,842 + BBR_RS12535 0.66 -1.8
Or see this region's nucleotide sequence