Experiment: CDM1 without Glycine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS19450 and BBR_RS19455 are separated by 66 nucleotides BBR_RS19455 and BBR_RS19460 are separated by 22 nucleotides BBR_RS19460 and BBR_RS19465 are separated by 186 nucleotides BBR_RS19465 and BBR_RS19470 are separated by 442 nucleotides
BBR_RS19450: BBR_RS19450 - GTPase ObgE, at 2,146,268 to 2,147,959
_RS19450
BBR_RS19455: BBR_RS19455 - 50S ribosomal protein L27, at 2,148,026 to 2,148,277
_RS19455
BBR_RS19460: BBR_RS19460 - 50S ribosomal protein L21, at 2,148,300 to 2,148,608
_RS19460
BBR_RS19465: BBR_RS19465 - ribonuclease E/G, at 2,148,795 to 2,151,857
_RS19465
BBR_RS19470: BBR_RS19470 - succinyl-diaminopimelate desuccinylase, at 2,152,300 to 2,153,505
_RS19470
Position (kb)
2148
2149
2150
2151
2152 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 2148.017 kb on - strand at 2148.017 kb on - strand at 2148.701 kb on + strand at 2148.723 kb on - strand at 2148.723 kb on - strand at 2148.734 kb on + strand at 2148.764 kb on + strand at 2148.772 kb on - strand at 2148.787 kb on + strand at 2148.787 kb on + strand at 2148.787 kb on + strand at 2148.795 kb on - strand at 2148.800 kb on + strand at 2148.833 kb on + strand at 2148.889 kb on - strand at 2150.700 kb on - strand, within BBR_RS19465 at 2151.027 kb on - strand, within BBR_RS19465 at 2151.285 kb on - strand, within BBR_RS19465 at 2151.435 kb on - strand, within BBR_RS19465 at 2151.483 kb on - strand, within BBR_RS19465 at 2151.495 kb on - strand, within BBR_RS19465 at 2151.593 kb on - strand at 2151.627 kb on - strand at 2151.627 kb on - strand at 2151.627 kb on - strand at 2151.708 kb on - strand at 2151.708 kb on - strand at 2151.899 kb on - strand at 2152.115 kb on + strand at 2152.115 kb on + strand at 2152.115 kb on + strand at 2152.123 kb on - strand at 2152.123 kb on - strand at 2152.125 kb on + strand at 2152.131 kb on + strand at 2152.131 kb on + strand at 2152.136 kb on + strand at 2152.137 kb on + strand at 2152.137 kb on + strand at 2152.139 kb on - strand at 2152.142 kb on + strand at 2152.160 kb on - strand at 2152.163 kb on + strand at 2152.167 kb on + strand at 2152.168 kb on + strand at 2152.171 kb on - strand at 2152.171 kb on - strand at 2152.171 kb on - strand at 2152.184 kb on + strand at 2152.186 kb on + strand at 2152.187 kb on + strand at 2152.187 kb on + strand at 2152.191 kb on + strand at 2152.192 kb on - strand at 2152.213 kb on + strand at 2152.214 kb on + strand at 2152.217 kb on + strand at 2152.220 kb on - strand at 2152.220 kb on - strand at 2152.221 kb on - strand at 2152.222 kb on - strand at 2152.237 kb on - strand at 2152.239 kb on + strand at 2152.239 kb on + strand at 2152.259 kb on - strand at 2152.259 kb on - strand at 2152.263 kb on - strand at 2152.273 kb on + strand at 2152.274 kb on + strand at 2152.281 kb on - strand at 2152.281 kb on - strand at 2152.281 kb on - strand at 2152.282 kb on - strand at 2152.306 kb on - strand at 2152.320 kb on - strand at 2152.344 kb on - strand at 2152.370 kb on - strand at 2152.370 kb on - strand at 2152.385 kb on - strand at 2152.389 kb on + strand at 2152.430 kb on - strand, within BBR_RS19470 at 2152.442 kb on - strand, within BBR_RS19470 at 2152.444 kb on + strand, within BBR_RS19470 at 2152.452 kb on - strand, within BBR_RS19470 at 2152.452 kb on - strand, within BBR_RS19470 at 2152.452 kb on - strand, within BBR_RS19470 at 2152.454 kb on + strand, within BBR_RS19470 at 2152.454 kb on + strand, within BBR_RS19470 at 2152.457 kb on - strand, within BBR_RS19470 at 2152.462 kb on - strand, within BBR_RS19470 at 2152.489 kb on + strand, within BBR_RS19470 at 2152.527 kb on + strand, within BBR_RS19470 at 2152.527 kb on + strand, within BBR_RS19470 at 2152.527 kb on + strand, within BBR_RS19470 at 2152.535 kb on - strand, within BBR_RS19470 at 2152.535 kb on - strand, within BBR_RS19470 at 2152.535 kb on - strand, within BBR_RS19470 at 2152.535 kb on - strand, within BBR_RS19470 at 2152.536 kb on + strand, within BBR_RS19470 at 2152.544 kb on - strand, within BBR_RS19470 at 2152.544 kb on - strand, within BBR_RS19470 at 2152.544 kb on - strand, within BBR_RS19470 at 2152.545 kb on - strand, within BBR_RS19470 at 2152.545 kb on - strand, within BBR_RS19470 at 2152.547 kb on + strand, within BBR_RS19470 at 2152.555 kb on - strand, within BBR_RS19470 at 2152.555 kb on - strand, within BBR_RS19470 at 2152.568 kb on + strand, within BBR_RS19470 at 2152.568 kb on + strand, within BBR_RS19470 at 2152.568 kb on + strand, within BBR_RS19470 at 2152.584 kb on + strand, within BBR_RS19470 at 2152.594 kb on + strand, within BBR_RS19470 at 2152.594 kb on + strand, within BBR_RS19470 at 2152.594 kb on - strand, within BBR_RS19470 at 2152.597 kb on - strand, within BBR_RS19470 at 2152.607 kb on - strand, within BBR_RS19470 at 2152.617 kb on + strand, within BBR_RS19470 at 2152.625 kb on - strand, within BBR_RS19470 at 2152.642 kb on + strand, within BBR_RS19470 at 2152.642 kb on - strand, within BBR_RS19470 at 2152.645 kb on - strand, within BBR_RS19470 at 2152.645 kb on - strand, within BBR_RS19470 at 2152.647 kb on - strand, within BBR_RS19470 at 2152.647 kb on - strand, within BBR_RS19470 at 2152.647 kb on - strand, within BBR_RS19470 at 2152.649 kb on + strand, within BBR_RS19470 at 2152.649 kb on + strand, within BBR_RS19470 at 2152.649 kb on + strand, within BBR_RS19470 at 2152.650 kb on - strand, within BBR_RS19470 at 2152.657 kb on - strand, within BBR_RS19470 at 2152.657 kb on - strand, within BBR_RS19470 at 2152.657 kb on - strand, within BBR_RS19470 at 2152.669 kb on - strand, within BBR_RS19470 at 2152.685 kb on + strand, within BBR_RS19470 at 2152.685 kb on + strand, within BBR_RS19470 at 2152.693 kb on - strand, within BBR_RS19470 at 2152.711 kb on + strand, within BBR_RS19470 at 2152.756 kb on + strand, within BBR_RS19470 at 2152.764 kb on + strand, within BBR_RS19470 at 2152.764 kb on + strand, within BBR_RS19470 at 2152.764 kb on - strand, within BBR_RS19470 at 2152.764 kb on - strand, within BBR_RS19470 at 2152.764 kb on - strand, within BBR_RS19470 at 2152.764 kb on - strand, within BBR_RS19470 at 2152.766 kb on + strand, within BBR_RS19470 at 2152.769 kb on + strand, within BBR_RS19470 at 2152.769 kb on - strand, within BBR_RS19470 at 2152.781 kb on - strand, within BBR_RS19470 at 2152.786 kb on - strand, within BBR_RS19470 at 2152.802 kb on - strand, within BBR_RS19470 at 2152.811 kb on + strand, within BBR_RS19470 at 2152.819 kb on - strand, within BBR_RS19470 at 2152.822 kb on + strand, within BBR_RS19470 at 2152.822 kb on + strand, within BBR_RS19470 at 2152.827 kb on + strand, within BBR_RS19470 at 2152.835 kb on - strand, within BBR_RS19470 at 2152.843 kb on + strand, within BBR_RS19470 at 2152.846 kb on - strand, within BBR_RS19470 at 2152.846 kb on - strand, within BBR_RS19470 at 2152.852 kb on + strand, within BBR_RS19470 at 2152.855 kb on + strand, within BBR_RS19470
Per-strain Table
Position Strand Gene LocusTag Fraction CDM1 without Glycine remove 2,148,017 - -1.5 2,148,017 - +2.1 2,148,701 + +0.4 2,148,723 - +1.3 2,148,723 - +1.1 2,148,734 + +0.9 2,148,764 + -1.7 2,148,772 - +4.0 2,148,787 + -1.8 2,148,787 + -0.1 2,148,787 + -2.9 2,148,795 - +0.7 2,148,800 + +2.6 2,148,833 + +1.8 2,148,889 - -1.8 2,150,700 - BBR_RS19465 0.62 -1.1 2,151,027 - BBR_RS19465 0.73 -1.9 2,151,285 - BBR_RS19465 0.81 -0.6 2,151,435 - BBR_RS19465 0.86 -1.1 2,151,483 - BBR_RS19465 0.88 -2.0 2,151,495 - BBR_RS19465 0.88 +1.1 2,151,593 - -2.9 2,151,627 - -1.3 2,151,627 - +3.5 2,151,627 - -0.2 2,151,708 - -0.7 2,151,708 - +1.1 2,151,899 - -1.0 2,152,115 + +3.0 2,152,115 + +0.7 2,152,115 + -0.5 2,152,123 - -2.8 2,152,123 - -0.8 2,152,125 + -2.5 2,152,131 + +1.3 2,152,131 + -1.0 2,152,136 + +1.8 2,152,137 + +1.8 2,152,137 + +2.0 2,152,139 - +0.8 2,152,142 + -0.4 2,152,160 - -1.8 2,152,163 + -1.7 2,152,167 + +3.3 2,152,168 + +1.7 2,152,171 - -1.5 2,152,171 - +3.0 2,152,171 - -1.7 2,152,184 + -1.5 2,152,186 + -0.5 2,152,187 + -1.6 2,152,187 + +1.4 2,152,191 + +3.7 2,152,192 - +0.5 2,152,213 + -1.3 2,152,214 + -1.8 2,152,217 + -2.3 2,152,220 - +0.9 2,152,220 - +3.5 2,152,221 - -0.7 2,152,222 - -2.6 2,152,237 - +0.8 2,152,239 + -2.2 2,152,239 + -1.1 2,152,259 - +2.2 2,152,259 - -1.4 2,152,263 - +1.7 2,152,273 + +4.8 2,152,274 + +1.1 2,152,281 - +1.6 2,152,281 - -1.2 2,152,281 - -0.2 2,152,282 - +2.5 2,152,306 - +4.2 2,152,320 - +1.5 2,152,344 - -0.7 2,152,370 - -2.0 2,152,370 - -2.6 2,152,385 - -2.8 2,152,389 + -0.7 2,152,430 - BBR_RS19470 0.11 -2.9 2,152,442 - BBR_RS19470 0.12 -1.1 2,152,444 + BBR_RS19470 0.12 -3.5 2,152,452 - BBR_RS19470 0.13 +0.3 2,152,452 - BBR_RS19470 0.13 -0.2 2,152,452 - BBR_RS19470 0.13 -1.6 2,152,454 + BBR_RS19470 0.13 -0.3 2,152,454 + BBR_RS19470 0.13 -2.6 2,152,457 - BBR_RS19470 0.13 -2.4 2,152,462 - BBR_RS19470 0.13 +1.1 2,152,489 + BBR_RS19470 0.16 -3.3 2,152,527 + BBR_RS19470 0.19 -0.3 2,152,527 + BBR_RS19470 0.19 -1.5 2,152,527 + BBR_RS19470 0.19 -1.3 2,152,535 - BBR_RS19470 0.19 -2.7 2,152,535 - BBR_RS19470 0.19 -3.1 2,152,535 - BBR_RS19470 0.19 +0.4 2,152,535 - BBR_RS19470 0.19 -0.5 2,152,536 + BBR_RS19470 0.20 -3.7 2,152,544 - BBR_RS19470 0.20 -1.7 2,152,544 - BBR_RS19470 0.20 -0.3 2,152,544 - BBR_RS19470 0.20 -0.6 2,152,545 - BBR_RS19470 0.20 -2.1 2,152,545 - BBR_RS19470 0.20 -0.3 2,152,547 + BBR_RS19470 0.20 -1.0 2,152,555 - BBR_RS19470 0.21 -2.0 2,152,555 - BBR_RS19470 0.21 -1.3 2,152,568 + BBR_RS19470 0.22 -2.2 2,152,568 + BBR_RS19470 0.22 -2.3 2,152,568 + BBR_RS19470 0.22 -2.2 2,152,584 + BBR_RS19470 0.24 -0.8 2,152,594 + BBR_RS19470 0.24 -3.2 2,152,594 + BBR_RS19470 0.24 -1.3 2,152,594 - BBR_RS19470 0.24 -1.3 2,152,597 - BBR_RS19470 0.25 -1.6 2,152,607 - BBR_RS19470 0.25 -1.0 2,152,617 + BBR_RS19470 0.26 -1.7 2,152,625 - BBR_RS19470 0.27 -2.3 2,152,642 + BBR_RS19470 0.28 -1.0 2,152,642 - BBR_RS19470 0.28 -0.6 2,152,645 - BBR_RS19470 0.29 -2.5 2,152,645 - BBR_RS19470 0.29 -2.6 2,152,647 - BBR_RS19470 0.29 -1.7 2,152,647 - BBR_RS19470 0.29 -1.7 2,152,647 - BBR_RS19470 0.29 -1.7 2,152,649 + BBR_RS19470 0.29 -1.3 2,152,649 + BBR_RS19470 0.29 -1.3 2,152,649 + BBR_RS19470 0.29 +0.1 2,152,650 - BBR_RS19470 0.29 -3.3 2,152,657 - BBR_RS19470 0.30 -1.8 2,152,657 - BBR_RS19470 0.30 -2.4 2,152,657 - BBR_RS19470 0.30 -1.1 2,152,669 - BBR_RS19470 0.31 -0.6 2,152,685 + BBR_RS19470 0.32 +1.3 2,152,685 + BBR_RS19470 0.32 -1.1 2,152,693 - BBR_RS19470 0.33 -2.7 2,152,711 + BBR_RS19470 0.34 -1.7 2,152,756 + BBR_RS19470 0.38 -2.7 2,152,764 + BBR_RS19470 0.38 -2.4 2,152,764 + BBR_RS19470 0.38 -2.6 2,152,764 - BBR_RS19470 0.38 -1.1 2,152,764 - BBR_RS19470 0.38 -0.3 2,152,764 - BBR_RS19470 0.38 -2.3 2,152,764 - BBR_RS19470 0.38 -2.3 2,152,766 + BBR_RS19470 0.39 -0.1 2,152,769 + BBR_RS19470 0.39 -0.7 2,152,769 - BBR_RS19470 0.39 -1.4 2,152,781 - BBR_RS19470 0.40 -0.7 2,152,786 - BBR_RS19470 0.40 -2.8 2,152,802 - BBR_RS19470 0.42 -0.9 2,152,811 + BBR_RS19470 0.42 -1.7 2,152,819 - BBR_RS19470 0.43 -0.9 2,152,822 + BBR_RS19470 0.43 +1.5 2,152,822 + BBR_RS19470 0.43 -0.7 2,152,827 + BBR_RS19470 0.44 -1.6 2,152,835 - BBR_RS19470 0.44 -2.2 2,152,843 + BBR_RS19470 0.45 -3.7 2,152,846 - BBR_RS19470 0.45 -2.4 2,152,846 - BBR_RS19470 0.45 -2.0 2,152,852 + BBR_RS19470 0.46 -2.5 2,152,855 + BBR_RS19470 0.46 -2.9
Or see this region's nucleotide sequence