Experiment: CDM1 without Glycine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS18600 and BBR_RS18605 are separated by 94 nucleotides BBR_RS18605 and BBR_RS21060 are separated by 205 nucleotides BBR_RS21060 and BBR_RS18610 are separated by 114 nucleotides BBR_RS18610 and BBR_RS18615 are separated by 83 nucleotides
BBR_RS18600: BBR_RS18600 - lysine--tRNA ligase, at 1,957,675 to 1,959,357
_RS18600
BBR_RS18605: BBR_RS18605 - tRNA-Pro, at 1,959,452 to 1,959,528
_RS18605
BBR_RS21060: BBR_RS21060 - hypothetical protein, at 1,959,734 to 1,959,991
_RS21060
BBR_RS18610: BBR_RS18610 - MFS transporter, at 1,960,106 to 1,961,296
_RS18610
BBR_RS18615: BBR_RS18615 - DUF4037 domain-containing protein, at 1,961,380 to 1,963,581
_RS18615
Position (kb)
1960
1961
1962 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 1959.365 kb on - strand at 1959.367 kb on - strand at 1959.385 kb on + strand at 1959.386 kb on - strand at 1959.629 kb on - strand at 1959.658 kb on + strand at 1959.660 kb on + strand at 1959.660 kb on + strand at 1959.660 kb on + strand at 1959.668 kb on - strand at 1959.683 kb on - strand at 1959.753 kb on - strand at 1959.765 kb on - strand, within BBR_RS21060 at 1959.784 kb on - strand, within BBR_RS21060 at 1959.794 kb on - strand, within BBR_RS21060 at 1959.794 kb on - strand, within BBR_RS21060 at 1959.832 kb on + strand, within BBR_RS21060 at 1959.835 kb on - strand, within BBR_RS21060 at 1959.867 kb on - strand, within BBR_RS21060 at 1959.867 kb on - strand, within BBR_RS21060 at 1959.880 kb on - strand, within BBR_RS21060 at 1959.930 kb on - strand, within BBR_RS21060 at 1959.930 kb on - strand, within BBR_RS21060 at 1960.013 kb on - strand at 1960.022 kb on + strand at 1960.022 kb on + strand at 1960.034 kb on + strand at 1960.034 kb on + strand at 1960.034 kb on + strand at 1960.074 kb on + strand at 1960.082 kb on - strand at 1960.119 kb on + strand at 1960.183 kb on + strand at 1960.189 kb on + strand at 1960.189 kb on + strand at 1960.213 kb on - strand at 1960.218 kb on - strand at 1960.218 kb on - strand at 1960.235 kb on + strand, within BBR_RS18610 at 1960.328 kb on + strand, within BBR_RS18610 at 1960.341 kb on + strand, within BBR_RS18610 at 1960.346 kb on + strand, within BBR_RS18610 at 1960.348 kb on + strand, within BBR_RS18610 at 1960.417 kb on - strand, within BBR_RS18610 at 1960.442 kb on - strand, within BBR_RS18610 at 1960.454 kb on + strand, within BBR_RS18610 at 1960.483 kb on + strand, within BBR_RS18610 at 1960.492 kb on - strand, within BBR_RS18610 at 1960.545 kb on - strand, within BBR_RS18610 at 1960.571 kb on + strand, within BBR_RS18610 at 1960.571 kb on + strand, within BBR_RS18610 at 1960.611 kb on + strand, within BBR_RS18610 at 1960.618 kb on + strand, within BBR_RS18610 at 1960.659 kb on - strand, within BBR_RS18610 at 1960.701 kb on + strand, within BBR_RS18610 at 1960.742 kb on + strand, within BBR_RS18610 at 1960.742 kb on + strand, within BBR_RS18610 at 1960.745 kb on - strand, within BBR_RS18610 at 1960.760 kb on + strand, within BBR_RS18610 at 1960.768 kb on + strand, within BBR_RS18610 at 1960.872 kb on + strand, within BBR_RS18610 at 1960.878 kb on - strand, within BBR_RS18610 at 1960.904 kb on + strand, within BBR_RS18610 at 1960.916 kb on + strand, within BBR_RS18610 at 1960.918 kb on - strand, within BBR_RS18610 at 1960.926 kb on - strand, within BBR_RS18610 at 1960.929 kb on + strand, within BBR_RS18610 at 1960.934 kb on + strand, within BBR_RS18610 at 1960.934 kb on + strand, within BBR_RS18610 at 1960.942 kb on - strand, within BBR_RS18610 at 1960.959 kb on - strand, within BBR_RS18610 at 1960.959 kb on - strand, within BBR_RS18610 at 1960.974 kb on - strand, within BBR_RS18610 at 1960.990 kb on - strand, within BBR_RS18610 at 1960.998 kb on - strand, within BBR_RS18610 at 1961.003 kb on - strand, within BBR_RS18610 at 1961.020 kb on - strand, within BBR_RS18610 at 1961.020 kb on - strand, within BBR_RS18610 at 1961.027 kb on + strand, within BBR_RS18610 at 1961.027 kb on + strand, within BBR_RS18610 at 1961.027 kb on + strand, within BBR_RS18610 at 1961.055 kb on + strand, within BBR_RS18610 at 1961.088 kb on + strand, within BBR_RS18610 at 1961.098 kb on - strand, within BBR_RS18610 at 1961.137 kb on - strand, within BBR_RS18610 at 1961.139 kb on + strand, within BBR_RS18610 at 1961.147 kb on - strand, within BBR_RS18610 at 1961.154 kb on - strand, within BBR_RS18610 at 1961.154 kb on - strand, within BBR_RS18610 at 1961.166 kb on + strand, within BBR_RS18610 at 1961.168 kb on - strand, within BBR_RS18610 at 1961.195 kb on + strand at 1961.195 kb on + strand at 1961.196 kb on + strand at 1961.204 kb on - strand at 1961.206 kb on + strand at 1961.211 kb on + strand at 1961.221 kb on + strand at 1961.226 kb on + strand at 1961.230 kb on - strand at 1961.230 kb on - strand at 1961.232 kb on + strand at 1961.232 kb on + strand at 1961.235 kb on - strand at 1961.240 kb on - strand at 1961.240 kb on - strand at 1961.254 kb on + strand at 1961.303 kb on + strand at 1961.311 kb on - strand at 1961.323 kb on - strand at 1961.325 kb on + strand at 1961.325 kb on + strand at 1961.326 kb on - strand at 1961.344 kb on - strand at 1961.345 kb on - strand at 1961.346 kb on - strand at 1961.346 kb on - strand at 1961.398 kb on - strand at 1961.432 kb on + strand at 1961.619 kb on + strand, within BBR_RS18615 at 1961.651 kb on + strand, within BBR_RS18615 at 1961.668 kb on + strand, within BBR_RS18615 at 1961.686 kb on + strand, within BBR_RS18615 at 1961.688 kb on + strand, within BBR_RS18615 at 1961.696 kb on - strand, within BBR_RS18615 at 1961.716 kb on - strand, within BBR_RS18615 at 1961.719 kb on + strand, within BBR_RS18615 at 1961.749 kb on - strand, within BBR_RS18615 at 1961.764 kb on + strand, within BBR_RS18615 at 1961.764 kb on + strand, within BBR_RS18615 at 1961.772 kb on - strand, within BBR_RS18615 at 1961.772 kb on - strand, within BBR_RS18615 at 1961.772 kb on - strand, within BBR_RS18615 at 1961.772 kb on - strand, within BBR_RS18615 at 1961.772 kb on - strand, within BBR_RS18615 at 1961.780 kb on + strand, within BBR_RS18615 at 1961.780 kb on + strand, within BBR_RS18615 at 1961.780 kb on + strand, within BBR_RS18615 at 1961.781 kb on + strand, within BBR_RS18615 at 1961.788 kb on - strand, within BBR_RS18615 at 1961.788 kb on - strand, within BBR_RS18615 at 1961.796 kb on - strand, within BBR_RS18615 at 1961.796 kb on - strand, within BBR_RS18615 at 1961.796 kb on - strand, within BBR_RS18615 at 1961.803 kb on + strand, within BBR_RS18615 at 1961.803 kb on - strand, within BBR_RS18615 at 1961.805 kb on + strand, within BBR_RS18615 at 1961.811 kb on - strand, within BBR_RS18615 at 1961.811 kb on - strand, within BBR_RS18615 at 1961.811 kb on - strand, within BBR_RS18615 at 1961.816 kb on + strand, within BBR_RS18615 at 1961.849 kb on + strand, within BBR_RS18615 at 1961.851 kb on + strand, within BBR_RS18615 at 1961.871 kb on + strand, within BBR_RS18615 at 1961.872 kb on + strand, within BBR_RS18615 at 1961.874 kb on - strand, within BBR_RS18615 at 1961.879 kb on - strand, within BBR_RS18615 at 1961.879 kb on - strand, within BBR_RS18615 at 1961.888 kb on + strand, within BBR_RS18615 at 1961.918 kb on - strand, within BBR_RS18615 at 1961.926 kb on + strand, within BBR_RS18615 at 1961.946 kb on + strand, within BBR_RS18615 at 1961.952 kb on - strand, within BBR_RS18615 at 1961.954 kb on - strand, within BBR_RS18615 at 1961.970 kb on + strand, within BBR_RS18615 at 1961.978 kb on - strand, within BBR_RS18615 at 1961.978 kb on - strand, within BBR_RS18615 at 1961.981 kb on + strand, within BBR_RS18615 at 1961.981 kb on + strand, within BBR_RS18615 at 1961.981 kb on + strand, within BBR_RS18615 at 1961.981 kb on + strand, within BBR_RS18615 at 1961.989 kb on - strand, within BBR_RS18615 at 1961.989 kb on - strand, within BBR_RS18615 at 1961.989 kb on - strand, within BBR_RS18615 at 1961.989 kb on - strand, within BBR_RS18615 at 1961.989 kb on - strand, within BBR_RS18615 at 1962.024 kb on - strand, within BBR_RS18615 at 1962.081 kb on + strand, within BBR_RS18615 at 1962.099 kb on + strand, within BBR_RS18615 at 1962.107 kb on - strand, within BBR_RS18615 at 1962.141 kb on + strand, within BBR_RS18615 at 1962.141 kb on + strand, within BBR_RS18615 at 1962.149 kb on - strand, within BBR_RS18615 at 1962.149 kb on - strand, within BBR_RS18615 at 1962.149 kb on - strand, within BBR_RS18615 at 1962.149 kb on - strand, within BBR_RS18615 at 1962.154 kb on + strand, within BBR_RS18615 at 1962.154 kb on + strand, within BBR_RS18615 at 1962.172 kb on + strand, within BBR_RS18615 at 1962.180 kb on - strand, within BBR_RS18615 at 1962.180 kb on - strand, within BBR_RS18615 at 1962.180 kb on - strand, within BBR_RS18615 at 1962.180 kb on - strand, within BBR_RS18615 at 1962.187 kb on - strand, within BBR_RS18615 at 1962.187 kb on - strand, within BBR_RS18615 at 1962.204 kb on + strand, within BBR_RS18615 at 1962.251 kb on + strand, within BBR_RS18615 at 1962.266 kb on + strand, within BBR_RS18615 at 1962.266 kb on + strand, within BBR_RS18615 at 1962.288 kb on + strand, within BBR_RS18615 at 1962.296 kb on - strand, within BBR_RS18615
Per-strain Table
Position Strand Gene LocusTag Fraction CDM1 without Glycine remove 1,959,365 - +2.7 1,959,367 - +2.5 1,959,385 + +1.8 1,959,386 - +0.6 1,959,629 - -1.6 1,959,658 + -2.1 1,959,660 + +1.1 1,959,660 + +1.3 1,959,660 + -1.8 1,959,668 - -0.3 1,959,683 - +1.3 1,959,753 - -0.6 1,959,765 - BBR_RS21060 0.12 +2.6 1,959,784 - BBR_RS21060 0.19 -1.5 1,959,794 - BBR_RS21060 0.23 +2.9 1,959,794 - BBR_RS21060 0.23 -0.3 1,959,832 + BBR_RS21060 0.38 +1.5 1,959,835 - BBR_RS21060 0.39 -1.7 1,959,867 - BBR_RS21060 0.52 -2.2 1,959,867 - BBR_RS21060 0.52 -2.5 1,959,880 - BBR_RS21060 0.57 -1.9 1,959,930 - BBR_RS21060 0.76 -1.3 1,959,930 - BBR_RS21060 0.76 +0.4 1,960,013 - -2.1 1,960,022 + -0.9 1,960,022 + -1.6 1,960,034 + +0.1 1,960,034 + +0.6 1,960,034 + +1.6 1,960,074 + -0.1 1,960,082 - -2.5 1,960,119 + +1.4 1,960,183 + -0.0 1,960,189 + -2.6 1,960,189 + +4.2 1,960,213 - -1.6 1,960,218 - -2.0 1,960,218 - +3.2 1,960,235 + BBR_RS18610 0.11 +0.7 1,960,328 + BBR_RS18610 0.19 +2.0 1,960,341 + BBR_RS18610 0.20 -2.7 1,960,346 + BBR_RS18610 0.20 +1.3 1,960,348 + BBR_RS18610 0.20 -3.3 1,960,417 - BBR_RS18610 0.26 -1.5 1,960,442 - BBR_RS18610 0.28 -0.9 1,960,454 + BBR_RS18610 0.29 -1.1 1,960,483 + BBR_RS18610 0.32 +3.5 1,960,492 - BBR_RS18610 0.32 -1.9 1,960,545 - BBR_RS18610 0.37 -0.2 1,960,571 + BBR_RS18610 0.39 -1.2 1,960,571 + BBR_RS18610 0.39 -1.6 1,960,611 + BBR_RS18610 0.42 -3.7 1,960,618 + BBR_RS18610 0.43 +2.6 1,960,659 - BBR_RS18610 0.46 -1.8 1,960,701 + BBR_RS18610 0.50 -0.5 1,960,742 + BBR_RS18610 0.53 -0.3 1,960,742 + BBR_RS18610 0.53 +0.5 1,960,745 - BBR_RS18610 0.54 -1.9 1,960,760 + BBR_RS18610 0.55 -2.1 1,960,768 + BBR_RS18610 0.56 +0.4 1,960,872 + BBR_RS18610 0.64 -2.6 1,960,878 - BBR_RS18610 0.65 -2.3 1,960,904 + BBR_RS18610 0.67 -1.2 1,960,916 + BBR_RS18610 0.68 -1.8 1,960,918 - BBR_RS18610 0.68 -2.6 1,960,926 - BBR_RS18610 0.69 +1.5 1,960,929 + BBR_RS18610 0.69 +2.4 1,960,934 + BBR_RS18610 0.70 -0.6 1,960,934 + BBR_RS18610 0.70 +1.7 1,960,942 - BBR_RS18610 0.70 -1.0 1,960,959 - BBR_RS18610 0.72 -2.0 1,960,959 - BBR_RS18610 0.72 -2.2 1,960,974 - BBR_RS18610 0.73 +1.6 1,960,990 - BBR_RS18610 0.74 -1.0 1,960,998 - BBR_RS18610 0.75 -1.5 1,961,003 - BBR_RS18610 0.75 +2.2 1,961,020 - BBR_RS18610 0.77 +2.1 1,961,020 - BBR_RS18610 0.77 +1.6 1,961,027 + BBR_RS18610 0.77 +3.6 1,961,027 + BBR_RS18610 0.77 -1.5 1,961,027 + BBR_RS18610 0.77 +0.0 1,961,055 + BBR_RS18610 0.80 -1.9 1,961,088 + BBR_RS18610 0.82 -2.0 1,961,098 - BBR_RS18610 0.83 -2.8 1,961,137 - BBR_RS18610 0.87 -1.3 1,961,139 + BBR_RS18610 0.87 +2.2 1,961,147 - BBR_RS18610 0.87 -0.2 1,961,154 - BBR_RS18610 0.88 +0.0 1,961,154 - BBR_RS18610 0.88 -2.8 1,961,166 + BBR_RS18610 0.89 -1.2 1,961,168 - BBR_RS18610 0.89 -1.1 1,961,195 + -2.6 1,961,195 + +2.9 1,961,196 + -1.3 1,961,204 - +1.7 1,961,206 + -1.2 1,961,211 + -1.4 1,961,221 + +2.3 1,961,226 + -1.4 1,961,230 - -2.6 1,961,230 - +2.8 1,961,232 + +2.7 1,961,232 + -3.8 1,961,235 - +2.5 1,961,240 - -0.2 1,961,240 - -2.1 1,961,254 + +2.4 1,961,303 + -0.9 1,961,311 - -1.1 1,961,323 - +2.8 1,961,325 + +0.7 1,961,325 + -0.8 1,961,326 - -1.5 1,961,344 - -2.5 1,961,345 - -2.3 1,961,346 - -0.5 1,961,346 - +1.6 1,961,398 - +0.1 1,961,432 + -2.1 1,961,619 + BBR_RS18615 0.11 +2.6 1,961,651 + BBR_RS18615 0.12 -2.3 1,961,668 + BBR_RS18615 0.13 +1.4 1,961,686 + BBR_RS18615 0.14 -1.6 1,961,688 + BBR_RS18615 0.14 -2.2 1,961,696 - BBR_RS18615 0.14 -2.2 1,961,716 - BBR_RS18615 0.15 +2.6 1,961,719 + BBR_RS18615 0.15 -3.2 1,961,749 - BBR_RS18615 0.17 -0.1 1,961,764 + BBR_RS18615 0.17 +2.7 1,961,764 + BBR_RS18615 0.17 +2.4 1,961,772 - BBR_RS18615 0.18 +0.6 1,961,772 - BBR_RS18615 0.18 +0.6 1,961,772 - BBR_RS18615 0.18 +3.4 1,961,772 - BBR_RS18615 0.18 -1.5 1,961,772 - BBR_RS18615 0.18 +2.8 1,961,780 + BBR_RS18615 0.18 -1.0 1,961,780 + BBR_RS18615 0.18 -0.4 1,961,780 + BBR_RS18615 0.18 +0.5 1,961,781 + BBR_RS18615 0.18 +0.7 1,961,788 - BBR_RS18615 0.19 -1.4 1,961,788 - BBR_RS18615 0.19 -1.9 1,961,796 - BBR_RS18615 0.19 +0.4 1,961,796 - BBR_RS18615 0.19 -1.8 1,961,796 - BBR_RS18615 0.19 +1.0 1,961,803 + BBR_RS18615 0.19 +1.6 1,961,803 - BBR_RS18615 0.19 +0.8 1,961,805 + BBR_RS18615 0.19 +3.3 1,961,811 - BBR_RS18615 0.20 +3.2 1,961,811 - BBR_RS18615 0.20 -1.1 1,961,811 - BBR_RS18615 0.20 +1.3 1,961,816 + BBR_RS18615 0.20 -1.2 1,961,849 + BBR_RS18615 0.21 -0.9 1,961,851 + BBR_RS18615 0.21 -1.7 1,961,871 + BBR_RS18615 0.22 -1.3 1,961,872 + BBR_RS18615 0.22 +0.4 1,961,874 - BBR_RS18615 0.22 +4.1 1,961,879 - BBR_RS18615 0.23 -1.8 1,961,879 - BBR_RS18615 0.23 +2.2 1,961,888 + BBR_RS18615 0.23 -1.0 1,961,918 - BBR_RS18615 0.24 +2.2 1,961,926 + BBR_RS18615 0.25 -0.1 1,961,946 + BBR_RS18615 0.26 +3.4 1,961,952 - BBR_RS18615 0.26 -1.2 1,961,954 - BBR_RS18615 0.26 -2.6 1,961,970 + BBR_RS18615 0.27 -0.9 1,961,978 - BBR_RS18615 0.27 -1.3 1,961,978 - BBR_RS18615 0.27 +1.1 1,961,981 + BBR_RS18615 0.27 -1.4 1,961,981 + BBR_RS18615 0.27 +0.2 1,961,981 + BBR_RS18615 0.27 -2.6 1,961,981 + BBR_RS18615 0.27 -1.8 1,961,989 - BBR_RS18615 0.28 +3.7 1,961,989 - BBR_RS18615 0.28 +0.5 1,961,989 - BBR_RS18615 0.28 +3.4 1,961,989 - BBR_RS18615 0.28 -1.3 1,961,989 - BBR_RS18615 0.28 -1.6 1,962,024 - BBR_RS18615 0.29 +2.8 1,962,081 + BBR_RS18615 0.32 -1.2 1,962,099 + BBR_RS18615 0.33 -1.2 1,962,107 - BBR_RS18615 0.33 -1.4 1,962,141 + BBR_RS18615 0.35 -1.5 1,962,141 + BBR_RS18615 0.35 +4.3 1,962,149 - BBR_RS18615 0.35 -2.2 1,962,149 - BBR_RS18615 0.35 +2.2 1,962,149 - BBR_RS18615 0.35 +2.0 1,962,149 - BBR_RS18615 0.35 +1.7 1,962,154 + BBR_RS18615 0.35 -1.4 1,962,154 + BBR_RS18615 0.35 +2.4 1,962,172 + BBR_RS18615 0.36 +3.8 1,962,180 - BBR_RS18615 0.36 -1.8 1,962,180 - BBR_RS18615 0.36 +2.8 1,962,180 - BBR_RS18615 0.36 -2.4 1,962,180 - BBR_RS18615 0.36 -2.6 1,962,187 - BBR_RS18615 0.37 -3.5 1,962,187 - BBR_RS18615 0.37 +2.0 1,962,204 + BBR_RS18615 0.37 +3.2 1,962,251 + BBR_RS18615 0.40 -1.6 1,962,266 + BBR_RS18615 0.40 -1.5 1,962,266 + BBR_RS18615 0.40 -1.0 1,962,288 + BBR_RS18615 0.41 +4.1 1,962,296 - BBR_RS18615 0.42 -1.4
Or see this region's nucleotide sequence