Experiment: CDM1 without Glycine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS17370 and BBR_RS17375 are separated by 18 nucleotides BBR_RS17375 and BBR_RS17380 are separated by 52 nucleotides BBR_RS17380 and BBR_RS17385 are separated by 203 nucleotides
BBR_RS17370: BBR_RS17370 - ABC transporter substrate-binding protein, at 1,675,129 to 1,675,968
_RS17370
BBR_RS17375: BBR_RS17375 - amino acid ABC transporter ATP-binding protein, at 1,675,987 to 1,676,829
_RS17375
BBR_RS17380: BBR_RS17380 - hypothetical protein, at 1,676,882 to 1,677,433
_RS17380
BBR_RS17385: BBR_RS17385 - aspartate--tRNA ligase, at 1,677,637 to 1,679,436
_RS17385
Position (kb)
1676
1677
1678 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 1675.895 kb on + strand at 1675.895 kb on + strand at 1675.898 kb on + strand at 1675.898 kb on + strand at 1675.905 kb on + strand at 1675.906 kb on - strand at 1675.931 kb on + strand at 1675.952 kb on - strand at 1675.988 kb on + strand at 1676.017 kb on - strand at 1676.057 kb on + strand at 1676.065 kb on - strand at 1676.119 kb on - strand, within BBR_RS17375 at 1676.142 kb on + strand, within BBR_RS17375 at 1676.225 kb on - strand, within BBR_RS17375 at 1676.240 kb on + strand, within BBR_RS17375 at 1676.246 kb on + strand, within BBR_RS17375 at 1676.246 kb on + strand, within BBR_RS17375 at 1676.248 kb on - strand, within BBR_RS17375 at 1676.248 kb on - strand, within BBR_RS17375 at 1676.272 kb on + strand, within BBR_RS17375 at 1676.386 kb on - strand, within BBR_RS17375 at 1676.386 kb on - strand, within BBR_RS17375 at 1676.472 kb on - strand, within BBR_RS17375 at 1676.489 kb on - strand, within BBR_RS17375 at 1676.503 kb on + strand, within BBR_RS17375 at 1676.503 kb on + strand, within BBR_RS17375 at 1676.511 kb on - strand, within BBR_RS17375 at 1676.515 kb on + strand, within BBR_RS17375 at 1676.525 kb on + strand, within BBR_RS17375 at 1676.615 kb on + strand, within BBR_RS17375 at 1676.662 kb on + strand, within BBR_RS17375 at 1676.704 kb on - strand, within BBR_RS17375 at 1676.722 kb on + strand, within BBR_RS17375 at 1676.760 kb on - strand at 1676.768 kb on + strand at 1676.853 kb on - strand at 1676.853 kb on - strand at 1676.867 kb on + strand at 1676.892 kb on + strand at 1676.938 kb on + strand, within BBR_RS17380 at 1677.007 kb on + strand, within BBR_RS17380 at 1677.078 kb on + strand, within BBR_RS17380 at 1677.078 kb on + strand, within BBR_RS17380 at 1677.094 kb on - strand, within BBR_RS17380 at 1677.094 kb on - strand, within BBR_RS17380 at 1677.096 kb on - strand, within BBR_RS17380 at 1677.119 kb on - strand, within BBR_RS17380 at 1677.127 kb on + strand, within BBR_RS17380 at 1677.135 kb on - strand, within BBR_RS17380 at 1677.135 kb on - strand, within BBR_RS17380 at 1677.137 kb on + strand, within BBR_RS17380 at 1677.145 kb on - strand, within BBR_RS17380 at 1677.160 kb on - strand, within BBR_RS17380 at 1677.175 kb on - strand, within BBR_RS17380 at 1677.197 kb on + strand, within BBR_RS17380 at 1677.197 kb on + strand, within BBR_RS17380 at 1677.223 kb on - strand, within BBR_RS17380 at 1677.241 kb on - strand, within BBR_RS17380 at 1677.254 kb on - strand, within BBR_RS17380 at 1677.281 kb on + strand, within BBR_RS17380 at 1677.289 kb on - strand, within BBR_RS17380 at 1677.289 kb on - strand, within BBR_RS17380 at 1677.289 kb on - strand, within BBR_RS17380 at 1677.346 kb on + strand, within BBR_RS17380 at 1677.354 kb on - strand, within BBR_RS17380 at 1677.360 kb on + strand, within BBR_RS17380 at 1677.371 kb on - strand, within BBR_RS17380 at 1677.384 kb on + strand at 1677.392 kb on - strand at 1677.392 kb on - strand at 1677.392 kb on - strand at 1677.397 kb on - strand at 1677.489 kb on - strand at 1677.490 kb on + strand at 1677.498 kb on - strand at 1677.498 kb on - strand at 1677.595 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction CDM1 without Glycine remove 1,675,895 + -1.7 1,675,895 + -2.2 1,675,898 + -2.1 1,675,898 + -1.9 1,675,905 + -1.8 1,675,906 - -2.0 1,675,931 + +1.6 1,675,952 - -0.2 1,675,988 + -2.1 1,676,017 - -1.0 1,676,057 + -2.2 1,676,065 - -2.4 1,676,119 - BBR_RS17375 0.16 -2.0 1,676,142 + BBR_RS17375 0.18 -1.5 1,676,225 - BBR_RS17375 0.28 -0.3 1,676,240 + BBR_RS17375 0.30 -1.8 1,676,246 + BBR_RS17375 0.31 -1.5 1,676,246 + BBR_RS17375 0.31 -1.8 1,676,248 - BBR_RS17375 0.31 -2.9 1,676,248 - BBR_RS17375 0.31 -3.4 1,676,272 + BBR_RS17375 0.34 -1.4 1,676,386 - BBR_RS17375 0.47 -1.2 1,676,386 - BBR_RS17375 0.47 -2.8 1,676,472 - BBR_RS17375 0.58 -1.3 1,676,489 - BBR_RS17375 0.60 -3.3 1,676,503 + BBR_RS17375 0.61 -0.9 1,676,503 + BBR_RS17375 0.61 +0.9 1,676,511 - BBR_RS17375 0.62 -3.1 1,676,515 + BBR_RS17375 0.63 -0.4 1,676,525 + BBR_RS17375 0.64 -2.9 1,676,615 + BBR_RS17375 0.74 -2.1 1,676,662 + BBR_RS17375 0.80 -0.4 1,676,704 - BBR_RS17375 0.85 -1.6 1,676,722 + BBR_RS17375 0.87 -2.8 1,676,760 - -1.6 1,676,768 + -2.6 1,676,853 - -1.3 1,676,853 - -1.0 1,676,867 + +0.5 1,676,892 + +4.1 1,676,938 + BBR_RS17380 0.10 +1.3 1,677,007 + BBR_RS17380 0.23 +1.1 1,677,078 + BBR_RS17380 0.36 +2.0 1,677,078 + BBR_RS17380 0.36 -1.4 1,677,094 - BBR_RS17380 0.38 -0.2 1,677,094 - BBR_RS17380 0.38 -0.2 1,677,096 - BBR_RS17380 0.39 -0.6 1,677,119 - BBR_RS17380 0.43 -0.7 1,677,127 + BBR_RS17380 0.44 +1.7 1,677,135 - BBR_RS17380 0.46 +4.6 1,677,135 - BBR_RS17380 0.46 -0.8 1,677,137 + BBR_RS17380 0.46 +4.3 1,677,145 - BBR_RS17380 0.48 -1.1 1,677,160 - BBR_RS17380 0.50 +1.4 1,677,175 - BBR_RS17380 0.53 +0.7 1,677,197 + BBR_RS17380 0.57 +1.9 1,677,197 + BBR_RS17380 0.57 -2.2 1,677,223 - BBR_RS17380 0.62 +3.4 1,677,241 - BBR_RS17380 0.65 +2.8 1,677,254 - BBR_RS17380 0.67 +2.3 1,677,281 + BBR_RS17380 0.72 +0.7 1,677,289 - BBR_RS17380 0.74 -0.6 1,677,289 - BBR_RS17380 0.74 +2.3 1,677,289 - BBR_RS17380 0.74 -1.7 1,677,346 + BBR_RS17380 0.84 +5.3 1,677,354 - BBR_RS17380 0.86 +3.9 1,677,360 + BBR_RS17380 0.87 +1.7 1,677,371 - BBR_RS17380 0.89 +1.2 1,677,384 + +0.2 1,677,392 - +3.8 1,677,392 - +1.6 1,677,392 - -2.1 1,677,397 - +0.4 1,677,489 - +2.7 1,677,490 + +2.8 1,677,498 - +2.5 1,677,498 - +1.4 1,677,595 + +2.6
Or see this region's nucleotide sequence