Experiment: CDM1 without Glycine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS14115 and BBR_RS14120 overlap by 4 nucleotides BBR_RS14120 and BBR_RS14125 are separated by 150 nucleotides
BBR_RS14115: BBR_RS14115 - transcription-repair coupling factor, at 941,761 to 945,351
_RS14115
BBR_RS14120: BBR_RS14120 - aldo/keto reductase, at 945,348 to 946,259
_RS14120
BBR_RS14125: BBR_RS14125 - phosphopyruvate hydratase, at 946,410 to 947,708
_RS14125
Position (kb) 945
946
947 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 944.354 kb on - strand, within BBR_RS14115 at 944.354 kb on - strand, within BBR_RS14115 at 944.416 kb on + strand, within BBR_RS14115 at 944.433 kb on + strand, within BBR_RS14115 at 944.488 kb on + strand, within BBR_RS14115 at 944.637 kb on - strand, within BBR_RS14115 at 944.652 kb on - strand, within BBR_RS14115 at 944.655 kb on + strand, within BBR_RS14115 at 944.701 kb on + strand, within BBR_RS14115 at 944.701 kb on + strand, within BBR_RS14115 at 944.755 kb on - strand, within BBR_RS14115 at 944.799 kb on + strand, within BBR_RS14115 at 944.821 kb on + strand, within BBR_RS14115 at 945.069 kb on + strand at 945.097 kb on - strand at 945.132 kb on + strand at 945.140 kb on - strand at 945.163 kb on - strand at 945.223 kb on + strand at 945.274 kb on + strand at 945.276 kb on - strand at 945.293 kb on + strand at 945.305 kb on + strand at 945.364 kb on + strand at 945.456 kb on + strand, within BBR_RS14120 at 945.464 kb on - strand, within BBR_RS14120 at 945.472 kb on - strand, within BBR_RS14120 at 945.474 kb on + strand, within BBR_RS14120 at 945.474 kb on + strand, within BBR_RS14120 at 945.479 kb on + strand, within BBR_RS14120 at 945.546 kb on + strand, within BBR_RS14120 at 945.614 kb on + strand, within BBR_RS14120 at 945.632 kb on + strand, within BBR_RS14120 at 945.657 kb on + strand, within BBR_RS14120 at 945.657 kb on + strand, within BBR_RS14120 at 945.665 kb on - strand, within BBR_RS14120 at 945.737 kb on + strand, within BBR_RS14120 at 945.782 kb on - strand, within BBR_RS14120 at 945.797 kb on - strand, within BBR_RS14120 at 945.797 kb on - strand, within BBR_RS14120 at 945.812 kb on - strand, within BBR_RS14120 at 945.840 kb on + strand, within BBR_RS14120 at 945.845 kb on + strand, within BBR_RS14120 at 945.845 kb on + strand, within BBR_RS14120 at 945.853 kb on - strand, within BBR_RS14120 at 945.943 kb on + strand, within BBR_RS14120 at 945.953 kb on + strand, within BBR_RS14120 at 945.979 kb on + strand, within BBR_RS14120 at 945.979 kb on + strand, within BBR_RS14120 at 945.979 kb on + strand, within BBR_RS14120 at 945.987 kb on - strand, within BBR_RS14120 at 945.989 kb on + strand, within BBR_RS14120 at 946.101 kb on - strand, within BBR_RS14120 at 946.101 kb on - strand, within BBR_RS14120 at 946.101 kb on - strand, within BBR_RS14120 at 946.125 kb on - strand, within BBR_RS14120 at 946.131 kb on - strand, within BBR_RS14120 at 946.268 kb on + strand at 946.337 kb on + strand at 946.345 kb on - strand at 946.346 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction CDM1 without Glycine remove 944,354 - BBR_RS14115 0.72 +0.5 944,354 - BBR_RS14115 0.72 +2.2 944,416 + BBR_RS14115 0.74 +1.5 944,433 + BBR_RS14115 0.74 +2.3 944,488 + BBR_RS14115 0.76 +1.8 944,637 - BBR_RS14115 0.80 +2.1 944,652 - BBR_RS14115 0.81 +1.5 944,655 + BBR_RS14115 0.81 -1.8 944,701 + BBR_RS14115 0.82 -1.0 944,701 + BBR_RS14115 0.82 +0.8 944,755 - BBR_RS14115 0.83 +1.1 944,799 + BBR_RS14115 0.85 -0.8 944,821 + BBR_RS14115 0.85 +1.8 945,069 + -1.1 945,097 - -0.0 945,132 + +4.1 945,140 - -1.1 945,163 - +1.1 945,223 + +2.4 945,274 + +0.9 945,276 - +2.2 945,293 + +3.2 945,305 + -0.8 945,364 + -2.1 945,456 + BBR_RS14120 0.12 -2.3 945,464 - BBR_RS14120 0.13 +4.2 945,472 - BBR_RS14120 0.14 -0.1 945,474 + BBR_RS14120 0.14 -1.3 945,474 + BBR_RS14120 0.14 +3.1 945,479 + BBR_RS14120 0.14 -1.3 945,546 + BBR_RS14120 0.22 +0.9 945,614 + BBR_RS14120 0.29 -2.1 945,632 + BBR_RS14120 0.31 -1.2 945,657 + BBR_RS14120 0.34 -1.7 945,657 + BBR_RS14120 0.34 -2.6 945,665 - BBR_RS14120 0.35 -2.3 945,737 + BBR_RS14120 0.43 -1.1 945,782 - BBR_RS14120 0.48 +3.5 945,797 - BBR_RS14120 0.49 -0.4 945,797 - BBR_RS14120 0.49 -1.0 945,812 - BBR_RS14120 0.51 +2.0 945,840 + BBR_RS14120 0.54 +0.3 945,845 + BBR_RS14120 0.54 +3.9 945,845 + BBR_RS14120 0.54 +0.8 945,853 - BBR_RS14120 0.55 +1.9 945,943 + BBR_RS14120 0.65 -1.6 945,953 + BBR_RS14120 0.66 -1.2 945,979 + BBR_RS14120 0.69 -0.3 945,979 + BBR_RS14120 0.69 -2.0 945,979 + BBR_RS14120 0.69 -2.6 945,987 - BBR_RS14120 0.70 -0.8 945,989 + BBR_RS14120 0.70 -2.4 946,101 - BBR_RS14120 0.83 -1.0 946,101 - BBR_RS14120 0.83 -3.3 946,101 - BBR_RS14120 0.83 +2.2 946,125 - BBR_RS14120 0.85 -1.8 946,131 - BBR_RS14120 0.86 -2.3 946,268 + +2.2 946,337 + -3.8 946,345 - -1.3 946,346 - -3.1
Or see this region's nucleotide sequence