Experiment: A22 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMc02766 and SMc02765 are separated by 5 nucleotides SMc02765 and SMc02764 are separated by 53 nucleotides SMc02764 and SMc02763 are separated by 15 nucleotides
SMc02766: SMc02766 - tryptophan synthase subunit beta, at 28,048 to 29,268
SMc02766
SMc02765: SMc02765 - tryptophan synthase subunit alpha, at 29,274 to 30,113
SMc02765
SMc02764: SMc02764 - acetyl-CoA carboxylase subunit beta, at 30,167 to 31,081
SMc02764
SMc02763: SMc02763 - bifunctional folylpolyglutamate synthase/dihydrofolate synthase, at 31,097 to 32,440
SMc02763
Position (kb)
30
31
32 Strain fitness (log2 ratio)
-1
0
1 at 29.266 kb on + strand at 29.267 kb on - strand at 29.341 kb on - strand at 29.580 kb on + strand, within SMc02765 at 29.584 kb on - strand, within SMc02765 at 29.584 kb on - strand, within SMc02765 at 29.595 kb on + strand, within SMc02765 at 29.595 kb on + strand, within SMc02765 at 29.595 kb on + strand, within SMc02765 at 29.595 kb on + strand, within SMc02765 at 29.595 kb on + strand, within SMc02765 at 29.595 kb on + strand, within SMc02765 at 29.595 kb on + strand, within SMc02765 at 29.596 kb on - strand, within SMc02765 at 29.596 kb on - strand, within SMc02765 at 29.596 kb on - strand, within SMc02765 at 29.596 kb on - strand, within SMc02765 at 29.596 kb on - strand, within SMc02765 at 29.597 kb on + strand, within SMc02765 at 29.597 kb on + strand, within SMc02765 at 29.597 kb on + strand, within SMc02765 at 29.598 kb on - strand, within SMc02765 at 29.598 kb on - strand, within SMc02765 at 29.598 kb on - strand, within SMc02765 at 29.601 kb on + strand, within SMc02765 at 29.602 kb on - strand, within SMc02765 at 29.602 kb on - strand, within SMc02765 at 29.602 kb on - strand, within SMc02765 at 29.602 kb on - strand, within SMc02765 at 29.799 kb on + strand, within SMc02765 at 29.802 kb on + strand, within SMc02765 at 29.802 kb on + strand, within SMc02765 at 29.803 kb on - strand, within SMc02765 at 29.803 kb on - strand, within SMc02765 at 29.885 kb on + strand, within SMc02765 at 29.886 kb on - strand, within SMc02765 at 29.886 kb on - strand, within SMc02765 at 31.084 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.02 mg/ml remove 29,266 + +0.9 29,267 - +1.0 29,341 - +1.6 29,580 + SMc02765 0.36 +0.1 29,584 - SMc02765 0.37 +0.4 29,584 - SMc02765 0.37 +0.5 29,595 + SMc02765 0.38 +0.5 29,595 + SMc02765 0.38 +0.0 29,595 + SMc02765 0.38 -0.1 29,595 + SMc02765 0.38 +0.7 29,595 + SMc02765 0.38 +0.2 29,595 + SMc02765 0.38 +0.7 29,595 + SMc02765 0.38 +0.4 29,596 - SMc02765 0.38 +1.0 29,596 - SMc02765 0.38 +0.8 29,596 - SMc02765 0.38 +0.6 29,596 - SMc02765 0.38 +0.3 29,596 - SMc02765 0.38 +0.9 29,597 + SMc02765 0.38 -0.4 29,597 + SMc02765 0.38 -0.4 29,597 + SMc02765 0.38 +0.3 29,598 - SMc02765 0.39 +0.3 29,598 - SMc02765 0.39 +0.1 29,598 - SMc02765 0.39 +0.5 29,601 + SMc02765 0.39 +0.1 29,602 - SMc02765 0.39 +0.0 29,602 - SMc02765 0.39 +0.5 29,602 - SMc02765 0.39 +0.5 29,602 - SMc02765 0.39 -0.7 29,799 + SMc02765 0.62 -0.8 29,802 + SMc02765 0.63 +0.3 29,802 + SMc02765 0.63 +0.2 29,803 - SMc02765 0.63 +0.7 29,803 - SMc02765 0.63 +0.5 29,885 + SMc02765 0.73 +0.3 29,886 - SMc02765 0.73 +0.5 29,886 - SMc02765 0.73 +0.4 31,084 + -0.1
Or see this region's nucleotide sequence