Experiment: A22 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMc01220 and SMc01219 are separated by 6 nucleotides SMc01219 and SMc01218 are separated by 83 nucleotides SMc01218 and SMc01217 are separated by 292 nucleotides
SMc01220: SMc01220 - lipopolysaccharide core biosynthesis glycosyl transferase, at 1,704,018 to 1,705,040
SMc01220
SMc01219: SMc01219 - lipopolysaccharide core biosynthesis mannosyltransferase, at 1,705,047 to 1,706,102
SMc01219
SMc01218: SMc01218 - transcription elongation factor GreA, at 1,706,186 to 1,706,662
SMc01218
SMc01217: SMc01217 - transport transmembrane protein, at 1,706,955 to 1,708,424
SMc01217
Position (kb)
1705
1706
1707 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1704.073 kb on + strand at 1704.074 kb on - strand at 1704.342 kb on + strand, within SMc01220 at 1704.361 kb on - strand, within SMc01220 at 1704.361 kb on - strand, within SMc01220 at 1704.442 kb on + strand, within SMc01220 at 1704.442 kb on + strand, within SMc01220 at 1704.443 kb on - strand, within SMc01220 at 1704.443 kb on - strand, within SMc01220 at 1704.569 kb on + strand, within SMc01220 at 1704.569 kb on + strand, within SMc01220 at 1704.570 kb on - strand, within SMc01220 at 1704.606 kb on + strand, within SMc01220 at 1705.011 kb on + strand at 1705.011 kb on + strand at 1705.011 kb on + strand at 1705.012 kb on - strand at 1706.775 kb on - strand at 1706.775 kb on - strand at 1706.775 kb on - strand at 1706.882 kb on - strand at 1706.882 kb on - strand at 1706.882 kb on - strand at 1706.892 kb on + strand at 1706.915 kb on + strand at 1706.915 kb on + strand at 1706.916 kb on - strand at 1706.933 kb on - strand at 1706.939 kb on + strand at 1706.940 kb on - strand at 1706.940 kb on - strand at 1706.962 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.02 mg/ml remove 1,704,073 + -3.6 1,704,074 - -4.0 1,704,342 + SMc01220 0.32 -3.0 1,704,361 - SMc01220 0.34 -4.2 1,704,361 - SMc01220 0.34 -4.7 1,704,442 + SMc01220 0.41 -4.0 1,704,442 + SMc01220 0.41 +0.2 1,704,443 - SMc01220 0.42 -4.2 1,704,443 - SMc01220 0.42 -3.4 1,704,569 + SMc01220 0.54 -3.8 1,704,569 + SMc01220 0.54 -3.1 1,704,570 - SMc01220 0.54 -2.8 1,704,606 + SMc01220 0.57 -2.5 1,705,011 + -4.7 1,705,011 + -3.8 1,705,011 + -4.7 1,705,012 - -3.5 1,706,775 - -0.0 1,706,775 - +0.4 1,706,775 - +0.3 1,706,882 - +0.3 1,706,882 - -0.5 1,706,882 - -1.4 1,706,892 + -1.1 1,706,915 + -0.2 1,706,915 + -1.0 1,706,916 - +0.1 1,706,933 - -0.5 1,706,939 + -0.4 1,706,940 - -0.5 1,706,940 - -0.2 1,706,962 + +1.2
Or see this region's nucleotide sequence