Experiment: A22 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa2139 and SMa2141 are separated by 93 nucleotides SMa2141 and SMa2143 overlap by 4 nucleotides
SMa2139: SMa2139 - SgaA serine-glyoxylate aminotransferase (SGAT), at 1,208,866 to 1,210,071
SMa2139
SMa2141: SMa2141 - formyltetrahydrofolate deformylase, at 1,210,165 to 1,211,025
SMa2141
SMa2143: SMa2143 - 5,10-methylenetetrahydrofolate reductase, at 1,211,022 to 1,211,975
SMa2143
Position (kb)
1210
1211
1212 Strain fitness (log2 ratio)
-1
0
1 at 1209.322 kb on + strand, within SMa2139 at 1209.339 kb on + strand, within SMa2139 at 1209.339 kb on + strand, within SMa2139 at 1209.339 kb on + strand, within SMa2139 at 1209.342 kb on - strand, within SMa2139 at 1209.376 kb on - strand, within SMa2139 at 1209.476 kb on - strand, within SMa2139 at 1210.059 kb on + strand at 1210.355 kb on + strand, within SMa2141 at 1210.355 kb on + strand, within SMa2141 at 1210.506 kb on - strand, within SMa2141 at 1210.608 kb on - strand, within SMa2141 at 1210.905 kb on - strand, within SMa2141 at 1210.934 kb on - strand, within SMa2141 at 1211.132 kb on + strand, within SMa2143 at 1211.133 kb on - strand, within SMa2143 at 1211.221 kb on - strand, within SMa2143 at 1211.221 kb on - strand, within SMa2143 at 1211.278 kb on - strand, within SMa2143 at 1211.435 kb on + strand, within SMa2143 at 1211.517 kb on - strand, within SMa2143 at 1211.647 kb on + strand, within SMa2143 at 1211.647 kb on + strand, within SMa2143 at 1211.648 kb on - strand, within SMa2143 at 1211.712 kb on - strand, within SMa2143 at 1211.848 kb on - strand, within SMa2143 at 1211.886 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.02 mg/ml remove 1,209,322 + SMa2139 0.38 +1.1 1,209,339 + SMa2139 0.39 -0.3 1,209,339 + SMa2139 0.39 +0.3 1,209,339 + SMa2139 0.39 +0.2 1,209,342 - SMa2139 0.39 +0.3 1,209,376 - SMa2139 0.42 +0.4 1,209,476 - SMa2139 0.51 +0.1 1,210,059 + +0.1 1,210,355 + SMa2141 0.22 +0.3 1,210,355 + SMa2141 0.22 +0.8 1,210,506 - SMa2141 0.40 +0.4 1,210,608 - SMa2141 0.51 -1.1 1,210,905 - SMa2141 0.86 +1.6 1,210,934 - SMa2141 0.89 +0.2 1,211,132 + SMa2143 0.12 -1.1 1,211,133 - SMa2143 0.12 -0.3 1,211,221 - SMa2143 0.21 +0.2 1,211,221 - SMa2143 0.21 +0.2 1,211,278 - SMa2143 0.27 +0.3 1,211,435 + SMa2143 0.43 +0.8 1,211,517 - SMa2143 0.52 +0.2 1,211,647 + SMa2143 0.66 -0.3 1,211,647 + SMa2143 0.66 +0.3 1,211,648 - SMa2143 0.66 +0.7 1,211,712 - SMa2143 0.72 -0.7 1,211,848 - SMa2143 0.87 +0.5 1,211,886 - -0.4
Or see this region's nucleotide sequence