Experiment: A22 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa1302 and SMa1303 are separated by 10 nucleotides SMa1303 and SMa1306 are separated by 9 nucleotides SMa1306 and SMa1308 overlap by 1 nucleotides
SMa1302: SMa1302 - VirB11-like ATPase, at 711,238 to 712,227
SMa1302
SMa1303: SMa1303 - VirB10-like transmembrane secretion protein, at 712,238 to 713,410
SMa1303
SMa1306: SMa1306 - VirB9 type IV secretion protein, at 713,420 to 714,274
SMa1306
SMa1308: SMa1308 - VirB8 type IV secretion protein, at 714,274 to 714,945
SMa1308
Position (kb) 712
713
714 Strain fitness (log2 ratio) -2
-1
0
1 at 711.252 kb on - strand at 711.301 kb on + strand at 711.301 kb on + strand at 711.313 kb on + strand at 711.314 kb on - strand at 711.426 kb on + strand, within SMa1302 at 711.427 kb on - strand, within SMa1302 at 711.431 kb on - strand, within SMa1302 at 711.431 kb on - strand, within SMa1302 at 711.468 kb on - strand, within SMa1302 at 711.470 kb on + strand, within SMa1302 at 711.470 kb on + strand, within SMa1302 at 711.471 kb on - strand, within SMa1302 at 711.471 kb on - strand, within SMa1302 at 711.588 kb on - strand, within SMa1302 at 711.592 kb on + strand, within SMa1302 at 711.593 kb on - strand, within SMa1302 at 711.593 kb on - strand, within SMa1302 at 711.619 kb on - strand, within SMa1302 at 711.789 kb on - strand, within SMa1302 at 711.789 kb on - strand, within SMa1302 at 712.085 kb on + strand, within SMa1302 at 712.086 kb on - strand, within SMa1302 at 712.135 kb on + strand at 712.135 kb on + strand at 712.148 kb on + strand at 712.240 kb on - strand at 712.240 kb on - strand at 712.329 kb on + strand at 712.329 kb on + strand at 712.330 kb on - strand at 712.330 kb on - strand at 712.330 kb on - strand at 712.506 kb on + strand, within SMa1303 at 712.506 kb on + strand, within SMa1303 at 712.507 kb on - strand, within SMa1303 at 712.578 kb on + strand, within SMa1303 at 712.578 kb on + strand, within SMa1303 at 712.711 kb on - strand, within SMa1303 at 712.838 kb on - strand, within SMa1303 at 713.062 kb on + strand, within SMa1303 at 713.064 kb on + strand, within SMa1303 at 713.065 kb on - strand, within SMa1303 at 713.199 kb on - strand, within SMa1303 at 713.422 kb on - strand at 713.422 kb on - strand at 713.479 kb on + strand at 713.483 kb on - strand at 713.508 kb on + strand, within SMa1306 at 713.518 kb on + strand, within SMa1306 at 713.576 kb on + strand, within SMa1306 at 713.643 kb on + strand, within SMa1306 at 713.643 kb on + strand, within SMa1306 at 713.643 kb on + strand, within SMa1306 at 713.644 kb on - strand, within SMa1306 at 713.644 kb on - strand, within SMa1306 at 713.703 kb on + strand, within SMa1306 at 713.766 kb on + strand, within SMa1306 at 713.766 kb on + strand, within SMa1306 at 713.874 kb on + strand, within SMa1306 at 713.884 kb on - strand, within SMa1306 at 713.889 kb on + strand, within SMa1306 at 713.944 kb on - strand, within SMa1306 at 713.947 kb on + strand, within SMa1306 at 713.987 kb on - strand, within SMa1306 at 714.153 kb on + strand, within SMa1306 at 714.154 kb on - strand, within SMa1306 at 714.162 kb on + strand, within SMa1306 at 714.162 kb on + strand, within SMa1306 at 714.163 kb on - strand, within SMa1306 at 714.163 kb on - strand, within SMa1306 at 714.266 kb on - strand at 714.368 kb on + strand, within SMa1308
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.02 mg/ml remove 711,252 - +0.3 711,301 + +0.4 711,301 + -0.1 711,313 + +0.2 711,314 - +0.3 711,426 + SMa1302 0.19 -0.2 711,427 - SMa1302 0.19 +0.4 711,431 - SMa1302 0.19 +0.3 711,431 - SMa1302 0.19 -0.5 711,468 - SMa1302 0.23 +0.6 711,470 + SMa1302 0.23 -0.6 711,470 + SMa1302 0.23 +0.3 711,471 - SMa1302 0.24 -2.5 711,471 - SMa1302 0.24 -0.3 711,588 - SMa1302 0.35 +0.4 711,592 + SMa1302 0.36 +0.2 711,593 - SMa1302 0.36 -1.3 711,593 - SMa1302 0.36 +0.2 711,619 - SMa1302 0.38 -0.1 711,789 - SMa1302 0.56 -0.5 711,789 - SMa1302 0.56 +0.2 712,085 + SMa1302 0.86 -0.2 712,086 - SMa1302 0.86 -0.2 712,135 + -0.3 712,135 + -1.0 712,148 + +0.2 712,240 - -1.4 712,240 - +0.6 712,329 + +0.2 712,329 + +0.1 712,330 - -0.5 712,330 - +0.1 712,330 - -0.1 712,506 + SMa1303 0.23 +0.0 712,506 + SMa1303 0.23 -0.1 712,507 - SMa1303 0.23 -0.7 712,578 + SMa1303 0.29 -1.5 712,578 + SMa1303 0.29 -0.5 712,711 - SMa1303 0.40 -0.5 712,838 - SMa1303 0.51 -0.2 713,062 + SMa1303 0.70 -0.4 713,064 + SMa1303 0.70 -0.2 713,065 - SMa1303 0.71 +0.8 713,199 - SMa1303 0.82 -0.5 713,422 - +0.6 713,422 - -0.0 713,479 + +0.6 713,483 - +0.1 713,508 + SMa1306 0.10 -0.0 713,518 + SMa1306 0.11 +0.2 713,576 + SMa1306 0.18 -0.5 713,643 + SMa1306 0.26 +0.4 713,643 + SMa1306 0.26 +1.3 713,643 + SMa1306 0.26 +0.6 713,644 - SMa1306 0.26 +1.0 713,644 - SMa1306 0.26 -0.5 713,703 + SMa1306 0.33 -0.2 713,766 + SMa1306 0.40 +0.8 713,766 + SMa1306 0.40 +0.1 713,874 + SMa1306 0.53 -0.6 713,884 - SMa1306 0.54 +1.3 713,889 + SMa1306 0.55 -0.1 713,944 - SMa1306 0.61 -0.9 713,947 + SMa1306 0.62 -0.2 713,987 - SMa1306 0.66 +0.0 714,153 + SMa1306 0.86 +0.8 714,154 - SMa1306 0.86 +0.2 714,162 + SMa1306 0.87 +0.0 714,162 + SMa1306 0.87 -1.4 714,163 - SMa1306 0.87 +0.5 714,163 - SMa1306 0.87 +0.0 714,266 - +0.3 714,368 + SMa1308 0.14 +0.5
Or see this region's nucleotide sequence