Experiment: A22 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa0831 and SMa0833 are separated by 542 nucleotides SMa0833 and SMa0834 overlap by 4 nucleotides SMa0834 and SMa5036 overlap by 29 nucleotides SMa5036 and SMa0835 are separated by 137 nucleotides
SMa0831: SMa0831 - NifX iron-molybdenum cluster-binding protein, at 459,663 to 460,163
SMa0831
SMa0833: SMa0833 - hypothetical protein, at 460,706 to 460,846
SMa0833
SMa0834: SMa0834 - FdxB ferredoxin III, at 460,843 to 461,160
SMa0834
SMa5036: SMa5036 - hypothetical protein, at 461,132 to 461,632
SMa5036
SMa0835: SMa0835 - hypothetical protein, at 461,770 to 462,060
SMa0835
Position (kb)
460
461 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 459.765 kb on - strand, within SMa0831 at 459.814 kb on - strand, within SMa0831 at 459.952 kb on + strand, within SMa0831 at 459.953 kb on - strand, within SMa0831 at 459.953 kb on - strand, within SMa0831 at 460.207 kb on + strand at 460.482 kb on - strand at 460.573 kb on + strand at 460.619 kb on - strand at 460.713 kb on - strand at 460.744 kb on + strand, within SMa0833 at 460.891 kb on + strand, within SMa0834 at 460.892 kb on - strand, within SMa0834 at 460.895 kb on + strand, within SMa0834 at 460.941 kb on + strand, within SMa0834 at 460.962 kb on + strand, within SMa0834 at 460.962 kb on + strand, within SMa0834 at 460.979 kb on + strand, within SMa0834 at 460.989 kb on - strand, within SMa0834 at 461.049 kb on + strand, within SMa0834 at 461.148 kb on - strand at 461.177 kb on + strand at 461.232 kb on + strand, within SMa5036 at 461.233 kb on - strand, within SMa5036 at 461.263 kb on + strand, within SMa5036 at 461.278 kb on + strand, within SMa5036 at 461.279 kb on - strand, within SMa5036 at 461.286 kb on + strand, within SMa5036 at 461.306 kb on - strand, within SMa5036 at 461.404 kb on - strand, within SMa5036 at 461.618 kb on - strand at 461.682 kb on - strand at 461.746 kb on + strand at 461.746 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.02 mg/ml remove 459,765 - SMa0831 0.20 +0.1 459,814 - SMa0831 0.30 -1.0 459,952 + SMa0831 0.58 -0.2 459,953 - SMa0831 0.58 +0.5 459,953 - SMa0831 0.58 -0.7 460,207 + -0.0 460,482 - -0.1 460,573 + -0.5 460,619 - +0.8 460,713 - -0.9 460,744 + SMa0833 0.27 -5.0 460,891 + SMa0834 0.15 +0.3 460,892 - SMa0834 0.15 -1.0 460,895 + SMa0834 0.16 -0.1 460,941 + SMa0834 0.31 -0.4 460,962 + SMa0834 0.37 +0.6 460,962 + SMa0834 0.37 +0.1 460,979 + SMa0834 0.43 +0.5 460,989 - SMa0834 0.46 +0.1 461,049 + SMa0834 0.65 -0.1 461,148 - -0.1 461,177 + -0.3 461,232 + SMa5036 0.20 -1.4 461,233 - SMa5036 0.20 -0.2 461,263 + SMa5036 0.26 +0.4 461,278 + SMa5036 0.29 +0.4 461,279 - SMa5036 0.29 +0.1 461,286 + SMa5036 0.31 +0.1 461,306 - SMa5036 0.35 -0.3 461,404 - SMa5036 0.54 -2.4 461,618 - -0.1 461,682 - +0.1 461,746 + -0.3 461,746 + -1.6
Or see this region's nucleotide sequence