Experiment: A22 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa0233 and SMa0235 are separated by 414 nucleotides SMa0235 and SMa0237 are separated by 8 nucleotides SMa0237 and SMa0241 overlap by 4 nucleotides
SMa0233: SMa0233 - OtsA trehalose-6-phosphate synthase, at 129,300 to 130,691
SMa0233
SMa0235: SMa0235 - dihydroxy-acid dehydratase, at 131,106 to 132,884
SMa0235
SMa0237: SMa0237 - dehydrogenase, at 132,893 to 133,774
SMa0237
SMa0241: SMa0241 - epimerase, at 133,771 to 134,754
SMa0241
Position (kb) 131
132
133 Strain fitness (log2 ratio) -1
0
1 at 130.144 kb on + strand, within SMa0233 at 130.194 kb on + strand, within SMa0233 at 130.289 kb on - strand, within SMa0233 at 130.453 kb on + strand, within SMa0233 at 130.495 kb on + strand, within SMa0233 at 130.495 kb on + strand, within SMa0233 at 130.496 kb on - strand, within SMa0233 at 130.502 kb on + strand, within SMa0233 at 130.718 kb on - strand at 131.019 kb on + strand at 131.020 kb on - strand at 131.108 kb on - strand at 131.108 kb on - strand at 131.127 kb on - strand at 131.591 kb on - strand, within SMa0235 at 131.660 kb on - strand, within SMa0235 at 131.778 kb on - strand, within SMa0235 at 131.929 kb on + strand, within SMa0235 at 132.170 kb on - strand, within SMa0235 at 132.535 kb on + strand, within SMa0235 at 132.565 kb on + strand, within SMa0235 at 132.692 kb on + strand, within SMa0235 at 132.692 kb on + strand, within SMa0235 at 132.692 kb on + strand, within SMa0235 at 132.693 kb on - strand, within SMa0235 at 132.805 kb on + strand at 132.806 kb on - strand at 132.825 kb on - strand at 132.825 kb on - strand at 133.375 kb on + strand, within SMa0237 at 133.504 kb on + strand, within SMa0237 at 133.504 kb on + strand, within SMa0237 at 133.504 kb on + strand, within SMa0237 at 133.504 kb on + strand, within SMa0237 at 133.505 kb on - strand, within SMa0237 at 133.505 kb on - strand, within SMa0237 at 133.519 kb on + strand, within SMa0237 at 133.589 kb on - strand, within SMa0237 at 133.685 kb on - strand, within SMa0237
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.02 mg/ml remove 130,144 + SMa0233 0.61 -0.2 130,194 + SMa0233 0.64 -0.1 130,289 - SMa0233 0.71 +0.2 130,453 + SMa0233 0.83 +0.6 130,495 + SMa0233 0.86 -0.5 130,495 + SMa0233 0.86 -1.2 130,496 - SMa0233 0.86 -0.1 130,502 + SMa0233 0.86 -0.4 130,718 - -0.6 131,019 + +0.4 131,020 - -0.0 131,108 - -0.5 131,108 - -1.5 131,127 - +0.4 131,591 - SMa0235 0.27 +0.4 131,660 - SMa0235 0.31 +0.3 131,778 - SMa0235 0.38 +0.2 131,929 + SMa0235 0.46 -0.0 132,170 - SMa0235 0.60 -0.1 132,535 + SMa0235 0.80 -0.4 132,565 + SMa0235 0.82 +0.1 132,692 + SMa0235 0.89 +0.6 132,692 + SMa0235 0.89 -0.7 132,692 + SMa0235 0.89 +0.0 132,693 - SMa0235 0.89 +0.1 132,805 + +0.7 132,806 - +0.1 132,825 - +0.8 132,825 - -0.2 133,375 + SMa0237 0.55 -0.9 133,504 + SMa0237 0.69 +0.5 133,504 + SMa0237 0.69 +0.0 133,504 + SMa0237 0.69 -0.2 133,504 + SMa0237 0.69 -0.1 133,505 - SMa0237 0.69 -0.2 133,505 - SMa0237 0.69 -1.4 133,519 + SMa0237 0.71 -1.1 133,589 - SMa0237 0.79 -0.4 133,685 - SMa0237 0.90 +0.6
Or see this region's nucleotide sequence