Experiment: Beta-Lactose (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS20195 and CA265_RS20200 are separated by 177 nucleotides CA265_RS20200 and CA265_RS20205 are separated by 250 nucleotides CA265_RS20205 and CA265_RS20210 are separated by 50 nucleotides CA265_RS20210 and CA265_RS20215 overlap by 29 nucleotides
CA265_RS20195: CA265_RS20195 - hypothetical protein, at 4,991,354 to 4,991,905
_RS20195
CA265_RS20200: CA265_RS20200 - nuclease, at 4,992,083 to 4,992,595
_RS20200
CA265_RS20205: CA265_RS20205 - very short patch repair endonuclease, at 4,992,846 to 4,993,349
_RS20205
CA265_RS20210: CA265_RS20210 - hypothetical protein, at 4,993,400 to 4,994,074
_RS20210
CA265_RS20215: CA265_RS20215 - transcriptional regulator, at 4,994,046 to 4,994,249
_RS20215
Position (kb)
4992
4993
4994 Strain fitness (log2 ratio)
-2
-1
0
1 at 4991.864 kb on + strand at 4991.909 kb on + strand at 4991.988 kb on - strand at 4991.991 kb on - strand at 4991.995 kb on + strand at 4992.077 kb on - strand at 4992.077 kb on - strand at 4992.078 kb on + strand at 4992.222 kb on + strand, within CA265_RS20200 at 4992.351 kb on + strand, within CA265_RS20200 at 4992.352 kb on - strand, within CA265_RS20200 at 4992.384 kb on + strand, within CA265_RS20200 at 4992.407 kb on - strand, within CA265_RS20200 at 4992.598 kb on + strand at 4992.606 kb on + strand at 4992.611 kb on + strand at 4992.615 kb on + strand at 4992.616 kb on - strand at 4992.656 kb on + strand at 4992.657 kb on - strand at 4992.682 kb on - strand at 4992.691 kb on - strand at 4992.691 kb on - strand at 4992.802 kb on - strand at 4992.840 kb on + strand at 4992.845 kb on + strand at 4992.919 kb on - strand, within CA265_RS20205 at 4992.924 kb on - strand, within CA265_RS20205 at 4992.928 kb on + strand, within CA265_RS20205 at 4992.928 kb on + strand, within CA265_RS20205 at 4992.957 kb on - strand, within CA265_RS20205 at 4992.989 kb on - strand, within CA265_RS20205 at 4993.028 kb on - strand, within CA265_RS20205 at 4993.058 kb on + strand, within CA265_RS20205 at 4993.070 kb on + strand, within CA265_RS20205 at 4993.070 kb on + strand, within CA265_RS20205 at 4993.070 kb on + strand, within CA265_RS20205 at 4993.071 kb on - strand, within CA265_RS20205 at 4993.071 kb on - strand, within CA265_RS20205 at 4993.188 kb on - strand, within CA265_RS20205 at 4993.233 kb on + strand, within CA265_RS20205 at 4993.236 kb on - strand, within CA265_RS20205 at 4993.303 kb on + strand at 4993.306 kb on - strand at 4993.310 kb on - strand at 4993.343 kb on + strand at 4993.344 kb on - strand at 4993.347 kb on + strand at 4993.347 kb on + strand at 4993.348 kb on - strand at 4993.373 kb on - strand at 4993.411 kb on - strand at 4993.412 kb on + strand at 4993.413 kb on - strand at 4993.415 kb on - strand at 4993.417 kb on - strand at 4993.454 kb on + strand at 4993.455 kb on - strand at 4993.525 kb on + strand, within CA265_RS20210 at 4993.530 kb on - strand, within CA265_RS20210 at 4993.552 kb on + strand, within CA265_RS20210 at 4993.633 kb on + strand, within CA265_RS20210 at 4993.634 kb on - strand, within CA265_RS20210 at 4993.634 kb on - strand, within CA265_RS20210 at 4993.635 kb on + strand, within CA265_RS20210 at 4993.636 kb on - strand, within CA265_RS20210 at 4993.636 kb on - strand, within CA265_RS20210 at 4993.709 kb on - strand, within CA265_RS20210 at 4993.746 kb on - strand, within CA265_RS20210 at 4993.838 kb on + strand, within CA265_RS20210 at 4993.857 kb on - strand, within CA265_RS20210 at 4993.885 kb on - strand, within CA265_RS20210 at 4993.891 kb on + strand, within CA265_RS20210 at 4993.892 kb on - strand, within CA265_RS20210 at 4993.896 kb on - strand, within CA265_RS20210 at 4993.904 kb on - strand, within CA265_RS20210 at 4994.008 kb on + strand at 4994.112 kb on + strand, within CA265_RS20215 at 4994.118 kb on - strand, within CA265_RS20215 at 4994.146 kb on + strand, within CA265_RS20215 at 4994.166 kb on + strand, within CA265_RS20215 at 4994.198 kb on + strand, within CA265_RS20215 at 4994.217 kb on + strand, within CA265_RS20215 at 4994.218 kb on - strand, within CA265_RS20215 at 4994.290 kb on - strand at 4994.296 kb on + strand at 4994.305 kb on + strand at 4994.306 kb on - strand at 4994.306 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Beta-Lactose (C) remove 4,991,864 + +0.6 4,991,909 + -1.1 4,991,988 - -0.3 4,991,991 - +1.0 4,991,995 + +0.2 4,992,077 - -1.4 4,992,077 - -1.0 4,992,078 + -0.8 4,992,222 + CA265_RS20200 0.27 +0.0 4,992,351 + CA265_RS20200 0.52 -0.6 4,992,352 - CA265_RS20200 0.52 -1.6 4,992,384 + CA265_RS20200 0.59 -2.6 4,992,407 - CA265_RS20200 0.63 -1.6 4,992,598 + -0.4 4,992,606 + -0.6 4,992,611 + +0.8 4,992,615 + +0.3 4,992,616 - +0.8 4,992,656 + -0.8 4,992,657 - +0.8 4,992,682 - +0.5 4,992,691 - -0.7 4,992,691 - -1.7 4,992,802 - +0.9 4,992,840 + -0.7 4,992,845 + +0.7 4,992,919 - CA265_RS20205 0.14 -2.5 4,992,924 - CA265_RS20205 0.15 +0.4 4,992,928 + CA265_RS20205 0.16 +0.2 4,992,928 + CA265_RS20205 0.16 +0.1 4,992,957 - CA265_RS20205 0.22 +1.2 4,992,989 - CA265_RS20205 0.28 +0.7 4,993,028 - CA265_RS20205 0.36 +0.7 4,993,058 + CA265_RS20205 0.42 +0.3 4,993,070 + CA265_RS20205 0.44 +1.0 4,993,070 + CA265_RS20205 0.44 -1.0 4,993,070 + CA265_RS20205 0.44 +0.6 4,993,071 - CA265_RS20205 0.45 +0.7 4,993,071 - CA265_RS20205 0.45 +0.1 4,993,188 - CA265_RS20205 0.68 +0.1 4,993,233 + CA265_RS20205 0.77 +0.4 4,993,236 - CA265_RS20205 0.77 +1.0 4,993,303 + +0.2 4,993,306 - +0.0 4,993,310 - -0.0 4,993,343 + -0.1 4,993,344 - +0.9 4,993,347 + -0.3 4,993,347 + -0.1 4,993,348 - +0.0 4,993,373 - +0.3 4,993,411 - +0.1 4,993,412 + +0.3 4,993,413 - +0.6 4,993,415 - -0.6 4,993,417 - -0.3 4,993,454 + +0.7 4,993,455 - +0.4 4,993,525 + CA265_RS20210 0.19 -1.3 4,993,530 - CA265_RS20210 0.19 -0.3 4,993,552 + CA265_RS20210 0.23 -1.2 4,993,633 + CA265_RS20210 0.35 -0.9 4,993,634 - CA265_RS20210 0.35 -0.1 4,993,634 - CA265_RS20210 0.35 +0.3 4,993,635 + CA265_RS20210 0.35 -0.1 4,993,636 - CA265_RS20210 0.35 +0.0 4,993,636 - CA265_RS20210 0.35 -1.5 4,993,709 - CA265_RS20210 0.46 +0.5 4,993,746 - CA265_RS20210 0.51 -0.1 4,993,838 + CA265_RS20210 0.65 +0.0 4,993,857 - CA265_RS20210 0.68 +0.0 4,993,885 - CA265_RS20210 0.72 +0.2 4,993,891 + CA265_RS20210 0.73 -0.2 4,993,892 - CA265_RS20210 0.73 -1.1 4,993,896 - CA265_RS20210 0.73 -0.8 4,993,904 - CA265_RS20210 0.75 +0.9 4,994,008 + +0.8 4,994,112 + CA265_RS20215 0.32 -0.1 4,994,118 - CA265_RS20215 0.35 +0.0 4,994,146 + CA265_RS20215 0.49 -0.3 4,994,166 + CA265_RS20215 0.59 -1.0 4,994,198 + CA265_RS20215 0.75 -1.3 4,994,217 + CA265_RS20215 0.84 -0.2 4,994,218 - CA265_RS20215 0.84 +0.1 4,994,290 - -0.3 4,994,296 + +0.7 4,994,305 + +0.4 4,994,306 - +1.5 4,994,306 - +0.3
Or see this region's nucleotide sequence